scholarly journals In vivo non-linear optical (NLO) imaging in live rabbit eyes using the Heidelberg Two-Photon Laser Ophthalmoscope

2010 ◽  
Vol 91 (2) ◽  
pp. 308-314 ◽  
Author(s):  
Ming Hao ◽  
Kevin Flynn ◽  
Chyong Nien-Shy ◽  
Bryan E. Jester ◽  
Moritz Winkler ◽  
...  
Keyword(s):  
Two Photon ◽  
Non Linear ◽  
Linear Optical

Second Harmonic Imaging Microscopy: A New Non-Linear Optical Modality for Cell Membrane Physiology

2000 ◽  
Vol 6 (S2) ◽  
pp. 810-811
Author(s):  
Paul Campagnola ◽  
Aaron Lewis ◽  
Leslie M. Loew
Keyword(s):  
Confocal Microscopy ◽  
Laser Scanning ◽  
Second Harmonic ◽  
Laser Scanning Microscopy ◽  
Scanning Microscopy ◽  
Photon Absorption ◽  
Photon Flux ◽  
Two Photon ◽  
Non Linear ◽  
Linear Optical

Confocal microscopy is an excellent high resolution method to image fluorescently labeled cells. However, the use of confocal microscopy to monitor physiological events, such as membrane potential changes, in living cells is hampered by photobleaching and phototoxicity. To reduce the collateral damage from excitation of fluorescent probes outside the optical slice, Webb and co-workers introduced the use of two-photon excited (TPE) fluorescence in laser scanning microscopy.1 Two-photon absorption depends on the square of the incident light intensity; this has the effect of confining excitation to the plane of focus where the photon flux density is greatest. The wavelength of the exciting light is in the near infrared facilitating penetration of thick tissues. Due to these significant advantages this methodology is rapidly gaining popularity as a tool for live cell and tissue imaging.To further exploit non-linear optical processes in laser scanning microscopy, we have developed surface second harmonic generation (SHG) as a powerful new imaging modality.

scholarly journals NON-LINEAR OPTICAL IMAGING OF OBESITY-RELATED HEALTH RISKS: REVIEW

2009 ◽  
Vol 02 (01) ◽  
pp. 9-25 ◽  
Author(s):  
THUC T. LE ◽  
JI-XIN CHENG
Keyword(s):  
Optical Imaging ◽  
Health Risks ◽  
Sum Frequency ◽  
Two Photon ◽  
Cars Microscopy ◽  
Non Linear ◽  
Linear Optical ◽  
Two Photon Fluorescence ◽  
Anti Stokes ◽  
Photon Fluorescence

This review highlights the recent applications of non-linear optical (NLO) microscopy to study obesity-related health risks. A strong emphasis is given to the applications of coherent anti-Stokes Raman scattering (CARS) microscopy where multiple non-linear optical imaging modalities including CARS, sum-frequency generation (SFG), and two-photon fluorescence are employed simultaneously on a single microscope platform. Specific examples on applications of NLO microscopy to study lipid-droplet biology, obesity-cancer relationship, atherosclerosis, and lipid-rich biological structures are discussed.

scholarly journals How To Extend The Capabilities Of A Commercial Two-Photon Microscope To Perform Super-Resolution Imaging, Wavelength Mixing And Label-Free Microscopy.

2021 ◽  
Author(s):  
Chiara Peres ◽  
Chiara Nardin ◽  
Guang Yang ◽  
Fabio Mammano
Keyword(s):  
Ex Vivo ◽  
Super Resolution ◽  
Label Free ◽  
Linear Optics ◽  
Non Linear Optics ◽  
Two Photon ◽  
Custom Made ◽  
Non Linear ◽  
Resolution Imaging

Multimodal microscopy combines multiple non-linear techniques that take advantage of different optical processes to generate contrast and increase the amount of information that can be obtained from biological samples. However, the most advanced optical architectures are typically custom-made and require complex alignment procedures, as well as daily maintenance by properly trained personnel for optimal performance. Here, we describe a hybrid system we constructed to overcome these disadvantages by modifying a commercial upright microscope. We show that our multimodal imaging platform can be used to seamlessly perform two-photon STED, wavelength mixing and label-free microscopy in both ex vivo and in vivo samples. The system is highly stable and endowed with remote alignment hardware that ensures simplified operability for non-expert users. This optical architecture is an important step forward towards a wider practical applicability of non-linear optics to bioimaging.

scholarly journals Photophysics of chlorin e6: from one- and two-photon absorption to fluorescence and phosphorescence

RSC Advances ◽  
2017 ◽  
Vol 7 (18) ◽  
pp. 10992-10999 ◽  
Author(s):  
Hugo Gattuso ◽  
Antonio Monari ◽  
Marco Marazzi
Keyword(s):  
Optical Properties ◽  
Singlet Oxygen ◽  
Photon Absorption ◽  
Chlorin E6 ◽  
Two Photon Absorption ◽  
Two Photon ◽  
Non Linear ◽  
Linear Optical Properties ◽  
Linear Optical ◽  
Fluorescence And Phosphorescence

Linear and non-linear optical properties of a known photosensitizer producing singlet oxygen, chlorin e6, have been studied, including dynamics effects.

In vivo imaging of liver injury and regeneration by functional two-photon microscopy

2016 ◽  
Vol 54 (12) ◽  
pp. 1343-1404
Author(s):  
A Ghallab ◽  
R Reif ◽  
R Hassan ◽  
AS Seddek ◽  
JG Hengstler
Keyword(s):  
Liver Injury ◽  
In Vivo Imaging ◽  
Two Photon Microscopy ◽  
Two Photon

Experimental and Theoretical (ab initio and DFT) Analysis of UV-Vis Spectra, Thermodynamic Functions and Non-linear Optical Properties of 2-Chloro-3,4-Dimethoxybenzaldehyde

2015 ◽  
Vol 8 (2) ◽  
pp. 2122-2134
Author(s):  
Sarvendra Kumar ◽  
Rajesh Kumar ◽  
Jayant Teotia ◽  
M. K. Yadav
Keyword(s):  
Thermodynamic Functions ◽  
Title Compound ◽  
Density Functional ◽  
Structural Characteristics ◽  
Basis Sets ◽  
Ultraviolet Absorption Spectrum ◽  
Non Linear ◽  
Different Temperatures ◽  
Linear Optical Properties ◽  
Linear Optical

In the present work, UV- Visible spectra of 2-Chloro-3,4-Dimethoxybenzaldehyde (2,3,4-CDMB) compound  have been carried out experimentally and theoretically. The ultraviolet absorption spectrum of title compound in three solvents (Acetone, Diethyl Ether, CCl4) of different polarity were examined in the range of 200–500 nm. The structure of the molecule was optimized and the structural characteristics were determined by HF and DFT (B3LYP) methods with 6-31+G(d,p) and 6-311++G(d,p) as basis sets. The excitation energy, wavelength corresponds to absorption maxima () and oscillator strength (f) are calculated by Time-Dependent Density Functional Theory (TD-DFT) using B3LYP/6-31+G(d,p) and B3LYP/6-311++G(d,p) as basis sets. The electric dipole moment (μ), polarizability (α) and the first hyperpolarizability (β ) have been computed to evaluate the non-linear optical (NLO) response of the investigated compound by HF and DFT (B3LYP) with already mentioned basis sets. Thermodynamic functions of the title compound at different temperatures were also calculated.

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