Schistosoma mansoni : In vivo evaluation of Phyllanthus amarus hexanic and ethanolic extracts

Objective: To evaluate the immunomodulatory activity of ethanolic extract of aerial parts of phyllanthus amarus plant in cyclophosphamide induced mice. Materials and Method: To evaluate the immunomodulatory activity of ethanolic extracts of Aerial parts of phyllanthus amarus plant. on cyclophosphamide induced immunosuppression in mice. ethanolic extracts of aerial parts of phyllanthus amarus plant, (500mg/kg p.o.) were administered 13 days to albino mice and cyclophosphamide (30mg/kg i.p.) was administered on 11th,12th and 13th days 1 hour after the administration of the respective treatment. On the 14th day blood was collected by retro orbital puncture and the activity was evaluated by determining the RBC, Hb%, Platelet, total WBC and differential counts. Ethanolic extracts of aerial parts phyllantus amarus plant. showed very significant (p<0.001) counteracting effect to cyclophosphamide induced reduction in total WBC, DLC and platelet counts & significant (P<0.01) effect to that of reduction in RBC counts and Hb %. The significant inmmunostimulant effect of the ethanolic extracts of aerial parts of phyllanthus amarus plant on cyclophosphamide induced myelosuppression may be attributed towards the collective presence of saponins, sterols and tannins in the extracts, which suggest the immunomodulatory activities of the ethanolic extracts of phyllanthusamarus plant, leaves,stem and fruits. Results: The results of the study revealed the dose dependent effect of theextracts in counteracting the myelosuppression induced by cyclophosphamide as inducated by increase in RBC total WBC platelet counts, Hb% and DLC in the extract treated group (III &IV), when compared to cyclophoshamide treated group (Group II). Conclusion: Ethanolic extract of phyllanthus amarus would contribute for significant immunostimulating activity of In-Vivo Keywords: Phyllanthus amarus, Cyclophosphamide, Immunomodulatory, Myelosuppression

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Avaliação in vitro e in vivo da atividade de frações e compostos isolados de Phyllanthus amarus contra o Schistosoma mansoni linhagem BH

10.47749/t/unicamp.2012.911027 ◽

2012 ◽

Author(s):

Claudineide Nascimento Fernandes Oliveira

Keyword(s):

Schistosoma Mansoni ◽

Phyllanthus Amarus

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Comparative Evaluation of Anthelmintic Activity of Edible and Ornamental Pomegranate Ethanolic Extracts againstSchistosoma mansoni

BioMed Research International ◽

10.1155/2016/2872708 ◽

2016 ◽

Vol 2016 ◽

pp. 1-15 ◽

Cited By ~ 4

Author(s):

Doaa A. Yones ◽

Dalia M. Badary ◽

Hesham M. B. Sayed ◽

Soad A. H. Bayoumi ◽

Azza A. Khalifa ◽

...

Keyword(s):

Schistosoma Mansoni ◽

Anthelmintic Activity ◽

Stem Bark ◽

Bark Extract ◽

Inflammatory Infiltration ◽

Ethanolic Extracts ◽

Good Potential ◽

Oxide Synthase

Due to the development of praziquantel (PZQ) schistosomes resistant strains, the discovery of new antischistosomal agents is of high priority in research. This work reported thein vitroandin vivoeffects of the edible and ornamental pomegranate extracts againstSchistosoma mansoni. Leaves and stem bark ethanolic extracts of both dried pomegranates were prepared at 100, 300, and 500 μg/mL forin vitroand 600 and 800 mg/kg forin vivo. Adult wormsSchistosoma mansoniin RPMI-1640 medium forin vitroandS. mansoniinfected mice forin vivotests were obtained from Theodor Bilharz Research Institute, Cairo, Egypt.In vitroactivity was manifested by significant coupled worms separation, reduction of motor activity, lethality, and ultrastructural tegumental alterations in adult worms.In vivoactivity was manifested revealed by significant reduction of hepatic granulomas number and diameter, decreased number of bilharzial eggs in liver tissues, lowered liver inflammatory infiltration, decreased hepatic fibrosis, and inducible nitric oxide synthase (iNOS) expression. Ethanolic stem bark extract of edible pomegranate exhibited highest antischistosomal activities bothin vitroandin vivo. Therefore, pomegranate showed a good potential to be used as a promising new candidate for the development of new schistosomicidal agents.

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