Regulation of cyclooxygenase activity in cultured endometrial stromal cells by granulocyte-macrophage colony-stimulating factor

2006 ◽  
Vol 85 ◽  
pp. 1118-1124 ◽  
Author(s):  
H WANG ◽  
Y WEN ◽  
M LAKEPOLAN ◽  
R BOOSTANFAR ◽  
M FEINMAN ◽  
...  
Keyword(s):  
Stromal Cells ◽  
Colony Stimulating Factor ◽  
Endometrial Stromal Cells ◽  
Cyclooxygenase Activity ◽  
Macrophage Colony Stimulating Factor ◽  
Macrophage Colony ◽  
Stimulating Factor

scholarly journals Premature expression of the macrophage colony-stimulating factor receptor on a multipotential stem cell line does not alter differentiation lineages controlled by stromal cells used for coculture.

1991 ◽  
Vol 173 (5) ◽  
pp. 1267-1279 ◽  
Author(s):  
T Kinashi ◽  
K H Lee ◽  
M Ogawa ◽  
K Tohyama ◽  
K Tashiro ◽  
...  
Keyword(s):  
Stem Cell ◽  
Stromal Cells ◽  
Signal Transduction Pathway ◽  
Transduction Pathway ◽  
Colony Stimulating Factor ◽  
Stem Cell Line ◽  
Gm Csf ◽  
Macrophage Colony Stimulating Factor ◽  
Macrophage Colony ◽  
Stimulating Factor

We are interested to know whether expression of a lineage-specific growth factor receptor is deterministic to lineage commitment during hematopoiesis. For this purpose, we introduced the human c-fms gene into the multipotential stem cell clone LyD9 and two myeloid progenitor clones, L-GM3 and L-G3, cells that differentiate in response to granulocyte/macrophage colony-stimulating factor (GM-CSF) and granulocyte (G)-CSF, respectively. Although LyD9 cells have differentiation potential to become macrophages, c-fms transfectants of LyD9 and L-GM3 cells did not differentiate in response to human macrophage (M)-CSF. However, c-fms transfectants of L-G3 cells differentiated to neutrophils in response to human M-CSF. These results indicate that the M-CSF receptor requires a specific signal transduction pathway to exert its differentiational and proliferative effects. Furthermore, the M-CSF receptor can convey a granulocyte-type differentiation signal possibly by cooperating with the G-CSF receptor signal transduction pathway. The c-fms-transfected LyD9 cells as well as the original LyD9 cells differentiated predominantly into GM-CSF- and G-CSF-responsive cells by coculturing with PA6 and ST2 stromal cells, respectively. The results indicate that differentiation lineage is not affected by premature expression of the M-CSF receptor. Instead, the stromal cell used for coculture apparently controls lineage-selective differentiation of the multi-potential stem cell line.

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