Differential expression and regulation of prostaglandin transporter and metabolic enzymes in mouse uterus during blastocyst implantation

2007 ◽  
Vol 88 (4) ◽  
pp. 1256-1265 ◽  
Author(s):  
Fei Gao ◽  
Wei Lei ◽  
Hong-Lu Diao ◽  
Shi-Jun Hu ◽  
Li-Ming Luan ◽  
...  
Keyword(s):  
Differential Expression ◽  
Metabolic Enzymes ◽  
Mouse Uterus ◽  
Blastocyst Implantation

scholarly journals Forkhead box a2 (FOXA2) is essential for uterine function and fertility

2017 ◽  
Vol 114 (6) ◽  
pp. E1018-E1026 ◽  
Author(s):  
Andrew M. Kelleher ◽  
Wang Peng ◽  
James K. Pru ◽  
Cindy A. Pru ◽  
Francesco J. DeMayo ◽  
...  
Keyword(s):  
Stromal Cell ◽  
Adult Mouse ◽  
Embryo Implantation ◽  
Critical Event ◽  
Mouse Uterus ◽  
Forkhead Box ◽  
Uterine Gland ◽  
Blastocyst Implantation ◽  
Uterine Function ◽  
Uterine Glands

Establishment of pregnancy is a critical event, and failure of embryo implantation and stromal decidualization in the uterus contribute to significant numbers of pregnancy losses in women. Glands of the uterus are essential for establishment of pregnancy in mice and likely in humans. Forkhead box a2 (FOXA2) is a transcription factor expressed specifically in the glands of the uterus and is a critical regulator of postnatal uterine gland differentiation in mice. In this study, we conditionally deleted FOXA2 in the adult mouse uterus using the lactotransferrin Cre (Ltf-Cre) model and in the neonatal mouse uterus using the progesterone receptor Cre (Pgr-Cre) model. The uteri of adult FOXA2-deleted mice were morphologically normal and contained glands, whereas the uteri of neonatal FOXA2-deleted mice were completely aglandular. Notably, adult FOXA2-deleted mice are completely infertile because of defects in blastocyst implantation and stromal cell decidualization. Leukemia inhibitory factor (LIF), a critical implantation factor of uterine gland origin, was not expressed during early pregnancy in adult FOXA2-deleted mice. Intriguingly, i.p. injections of LIF initiated blastocyst implantation in the uteri of both gland-containing and glandless adult FOXA2-deleted mice. Although pregnancy was rescued by LIF and was maintained to term in uterine gland-containing adult FOXA2-deleted mice, pregnancy failed by day 10 in neonatal FOXA2-deleted mice lacking uterine glands. These studies reveal a previously unrecognized role for FOXA2 in regulation of adult uterine function and fertility and provide original evidence that uterine glands and, by inference, their secretions play important roles in blastocyst implantation and stromal cell decidualization.

Differential expression of transcriptional repressor snail gene at implantation site in mouse uterus

2006 ◽  
Vol 73 (2) ◽  
pp. 133-141 ◽  
Author(s):  
Xing-Hong Ma ◽  
Shi-Jun Hu ◽  
Hao Yu ◽  
Li-Bin Xu ◽  
Zeng-Ming Yang
Keyword(s):  
Differential Expression ◽  
Transcriptional Repressor ◽  
Implantation Site ◽  
Mouse Uterus ◽  
Snail Gene

scholarly journals Differential expression and regulation of Tdo2 during mouse decidualization

2013 ◽  
Vol 220 (1) ◽  
pp. 73-83 ◽  
Author(s):  
Dang-Dang Li ◽  
Ying-Jie Gao ◽  
Xue-Chao Tian ◽  
Zhan-Qing Yang ◽  
Hang Cao ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Differential Expression ◽  
Stromal Cells ◽  
Real Time Pcr ◽  
Mouse Uterus ◽  
Decidual Cells ◽  
High Level ◽  
Rate Limiting

Tryptophan 2,3-dioxygenase (Tdo2) is a rate-limiting enzyme which directs the conversion of tryptophan to kynurenine. The aim of this study was to examine the expression and regulation of Tdo2 in mouse uterus during decidualization. Tdo2 mRNA was mainly expressed in the decidua on days 6–8 of pregnancy. By real-time PCR, a high level of Tdo2 expression was observed in the uteri from days 6 to 8 of pregnancy, although Tdo2 expression was observed on days 1–8. Simultaneously, Tdo2 mRNA was also detected under in vivo and in vitro artificial decidualization. Estrogen, progesterone, and 8-bromoadenosine-cAMP could induce the expression of Tdo2 in the ovariectomized mouse uterus and uterine stromal cells. Tdo2 could regulate cell proliferation and stimulate the expression of decidual marker Dtprp in the uterine stromal cells and decidual cells. Overexpression of Tdo2 could upregulate the expression of Ahr, Cox2, and Vegf genes in uterine stromal cells, while Tdo2 inhibitor 680C91 could downregulate the expression of Cox2 and Vegf genes in uterine decidual cells. These data indicate that Tdo2 may play an important role during mouse decidualization and be regulated by estrogen, progesterone, and cAMP.

scholarly journals Differential expression and anti-oxidant function of glutathione peroxidase 3 in mouse uterus during decidualization

FEBS Letters ◽  
2014 ◽  
Vol 588 (9) ◽  
pp. 1580-1589 ◽  
Author(s):  
Xiu Xu ◽  
Jing-Yu Leng ◽  
Fei Gao ◽  
Zhen-Ao Zhao ◽  
Wen-Bo Deng ◽  
...  
Keyword(s):  
Glutathione Peroxidase ◽  
Differential Expression ◽  
Mouse Uterus ◽  
Glutathione Peroxidase 3 ◽  
Anti Oxidant

Differential expression and regulation of Ido2 in the mouse uterus during peri-implantation period

2014 ◽  
Vol 51 (3) ◽  
pp. 264-272 ◽  
Author(s):  
Dang-Dang Li ◽  
Xin-Yuan Liu ◽  
Chuan-Hui Guo ◽  
Liang Yue ◽  
Zhan-Qing Yang ◽  
...  
Keyword(s):  
Differential Expression ◽  
Mouse Uterus ◽  
Implantation Period

scholarly journals Differential Expression of the Motin Family in the Peri-implantation Mouse Uterus and Their Hormonal Regulation

2012 ◽  
Vol 58 (6) ◽  
pp. 649-653 ◽  
Author(s):  
Hiromichi MATSUMOTO ◽  
Emiko FUKUI ◽  
Midori YOSHIZAWA ◽  
Eimei SATO ◽  
Takiko DAIKOKU
Keyword(s):  
Differential Expression ◽  
Hormonal Regulation ◽  
Mouse Uterus

Regulation of expression of the retinoic acid-synthesising enzymes retinaldehyde dehydrogenases in the uteri of ovariectomised mice after treatment with oestrogen, gestagen and their combination

2006 ◽  
Vol 18 (3) ◽  
pp. 339 ◽  
Author(s):  
Ralph Rühl ◽  
Britta Fritzsche ◽  
Julien Vermot ◽  
Karen Niederreither ◽  
Ulrike Neumann ◽  
...  
Keyword(s):  
Retinoic Acid ◽  
Early Pregnancy ◽  
Expression Patterns ◽  
In Situ Hybridisation ◽  
Oestrous Cycle ◽  
Regulation Of Expression ◽  
Mouse Uterus ◽  
Blastocyst Implantation ◽  
Polymerase Chain

The active metabolite of vitamin A, retinoic acid (RA), plays an important role in the female reproductive system. The synthesis of RA is tightly regulated by the activity of retinaldehyde dehydrogenases (Raldh). Among these, Raldh1 and Raldh2 exhibit specific temporal and spatial expression patterns in the mouse uterus, both during the oestrous cycle and early pregnancy. In the present study, we have assessed whether oestradiol and progesterone directly influence the uterine expression of Raldh1 and Raldh2 in ovariectomised mice. We investigated the effect of gestagen (promegestone 0.3 mg kg−1 bodyweight), oestrogen (oestradiol 3 µg kg−1 bodyweight) and their combination on the uterine expression of Raldh2. Expression was analysed using in situ hybridisation and quantified using real-time detection reverse transcription–polymerase chain reaction. The results show that the expression of Raldh2 is rapidly (within 1–4 h) induced in stromal cells by oestrogen, but not by gestagen, treatment, whereas combined oestrogen + gestagen treatment leads to a more prolonged (48 h) response. In contrast, oestrogen, but not progesterone, treatment downregulates (within 4–24 h) Raldh1 expression in the uterine glandular epithelium. We conclude that the uterine RA concentrations are regulated by oestrogens via an effect on the expression of the Raldh synthesising enzymes. Such a regulation is consistent with the natural fluctuations of Raldh expression during the oestrous cycle, early pregnancy and blastocyst implantation.

ID gene family exhibits a differential expression in mouse uterus and preimplantation embryos

2003 ◽  
Vol 80 ◽  
pp. 282-283
Author(s):  
HeeYoung Nah ◽  
SeokHo Hong ◽  
JiYoon Lee ◽  
ChungHoon Kim
Keyword(s):  
Gene Family ◽  
Differential Expression ◽  
Preimplantation Embryos ◽  
Mouse Uterus
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