Sperm viability, apoptosis, and intracellular reactive oxygen species levels in human spermatozoa before and after induction of oxidative stress

2010 ◽  
Vol 93 (3) ◽  
pp. 814-821 ◽  
Author(s):  
Reda Z. Mahfouz ◽  
Stefan S. du Plessis ◽  
Nabil Aziz ◽  
Rakesh Sharma ◽  
Edmund Sabanegh ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Reactive Oxygen Species ◽  
Reactive Oxygen ◽  
Intracellular Reactive Oxygen Species ◽  
Human Spermatozoa ◽  
Oxygen Species ◽  
Sperm Viability ◽  
Before And After

scholarly journals Ginkgo biloba extract (EGb 761) attenuates oxidative stress induction in erythrocytes of sickle cell disease patients

2012 ◽  
Vol 48 (4) ◽  
pp. 659-665 ◽  
Author(s):  
Aline Emmer Ferreira Furman ◽  
Railson Henneberg ◽  
Priscila Bacarin Hermann ◽  
Maria Suely Soares Leonart ◽  
Aguinaldo José do Nascimento
Keyword(s):  
Oxidative Stress ◽  
Reactive Oxygen Species ◽  
Lipid Peroxidation ◽  
Sickle Cell ◽  
Reduced Glutathione ◽  
Reactive Oxygen ◽  
Intracellular Reactive Oxygen Species ◽  
Oxygen Species ◽  
Egb 761 ◽  
Cell Disease

Sickle cell disease promotes hemolytic anemia and occlusion of small blood vessels due to the presence of high concentrations of hemoglobin S, resulting in increased production of reactive oxygen species and decreased antioxidant defense capacity. The aim of this study was to evaluate the protective action of a standardized extract of Ginkgo biloba (EGb 761), selected due to its high content of flavonoids and terpenoids, in erythrocytes of patients with sickle cell anemia (HbSS, SS erythrocytes) subjected to oxidative stress using tert-butylhydroperoxide or 2,2-azobis-(amidinepropane)-dihydrochloride, in vitro. Hemolysis indexes, reduced glutathione, methemoglobin concentrations, lipid peroxidation, and intracellular reactive oxygen species were determined. SS erythrocytes displayed increased rates of oxidation of hemoglobin and membrane lipid peroxidation compared to normal erythrocytes (HbAA, AA erythrocytes), and the concentration of EGb 761 necessary to achieve the same antioxidant effect in SS erythrocytes was at least two times higher than in normal ones, inhibiting the formation of intracellular reactive oxygen species (IC50 of 13.6 µg/mL), partially preventing lipid peroxidation (IC50 of 242.5 µg/mL) and preventing hemolysis (IC50 of 10.5 µg/mL). Thus, EGb 761 has a beneficial effect on the oxidative status of SS erythrocytes. Moreover, EGb 761 failed to prevent oxidation of hemoglobin and reduced glutathione at the concentrations examined.

scholarly journals Effects of ROS in human adrenocortical carcinoma SW-13 cells

2018 ◽  
Author(s):  
Eriko Shimada ◽  
Yusuke Tsuruwaka
Keyword(s):  
Oxidative Stress ◽  
Reactive Oxygen Species ◽  
Cell Proliferation ◽  
Cell Differentiation ◽  
Adrenocortical Carcinoma ◽  
Reactive Oxygen ◽  
Intracellular Reactive Oxygen Species ◽  
Oxygen Species ◽  
Effective Contact ◽  
Intracellular Ca

Paraquat is a highly effective contact herbicide, an inducer of reactive oxygen species (ROS), which generate oxidative stress in cells. Reactive oxygen species has been known to function as an important messenger in cell differentiation. In this study, we investigated the effect of paraquat on cell differentiation using human adenocortical carcinoma (ACC) SW-13 cells. Paraquat, an inducer of intracellular reactive oxygen species, induced the differentiation of SW-13 cells into neural-like cells in the presence of neurofilament. Moreover, paraquat exposure promoted SW-13 cell proliferation and increased intracellular Ca2+ concentration.

scholarly journals Effects of ROS in human adrenocortical carcinoma SW-13 cells

2018 ◽  
Author(s):  
Eriko Shimada ◽  
Yusuke Tsuruwaka
Keyword(s):  
Oxidative Stress ◽  
Reactive Oxygen Species ◽  
Cell Proliferation ◽  
Cell Differentiation ◽  
Adrenocortical Carcinoma ◽  
Reactive Oxygen ◽  
Intracellular Reactive Oxygen Species ◽  
Oxygen Species ◽  
Effective Contact ◽  
Intracellular Ca

Paraquat is a highly effective contact herbicide, an inducer of reactive oxygen species (ROS), which generate oxidative stress in cells. Reactive oxygen species has been known to function as an important messenger in cell differentiation. In this study, we investigated the effect of paraquat on cell differentiation using human adenocortical carcinoma (ACC) SW-13 cells. Paraquat, an inducer of intracellular reactive oxygen species, induced the differentiation of SW-13 cells into neural-like cells in the presence of neurofilament. Moreover, paraquat exposure promoted SW-13 cell proliferation and increased intracellular Ca2+ concentration.

Therapeutic Reactive Oxygen Generation

2008 ◽  
Vol 94 (2) ◽  
pp. 278-283 ◽  
Author(s):  
Peter Scharff ◽  
Uwe Ritter ◽  
Olga P Matyshevska ◽  
Svitlana V Prylutska ◽  
Iryna I Grynyuk ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Reactive Oxygen Species ◽  
Reactive Oxygen ◽  
Intracellular Reactive Oxygen Species ◽  
Transformed Cells ◽  
Oxygen Species ◽  
Selective Activation ◽  
Oxygen Generation ◽  
Cell Components ◽  
Nanosilica Particles

An increase of the intracellular reactive oxygen species (ROS) concentration leads to the development of oxidative stress and, thus, to the damage of cell components. The cause-and-effect relations between these processes have not been fully established yet. The ability of photo excited supramolecular composites containing fullerenes C60 immobilized at nanosilica particles to generate reactive oxygen species (ROS) in cells of two types (rat thymocytes, and transformed cells of ascite Erlich carcinoma, EAC, and leucosis L1210) is demonstrated. The damaging effect of photo excited C60-composites are shown, which appeared to be selective and manifested in transformed cells, but not in thymocytes. It has been shown that after the irradiation of aqueous solutions or cell suspensions in the presence of fullerene C60, the generation of reactive oxygen species is observed. It has been shown that the influence of photo excited fullerene C60 on metabolic processes depends on the composition of C60-containing complex and on the type of the cells. The damaging effects of photo excited fullerene C60-containing composites were demonstrated to be selective. The data presented suggest that the application of fullerene C60-containing composites for the selective activation of ROS-dependent death program in certain types of tumor cells is very promising.

scholarly journals Significance of Mitochondrial Reactive Oxygen Species in the Generation of Oxidative Stress in Spermatozoa

2008 ◽  
Vol 93 (8) ◽  
pp. 3199-3207 ◽  
Author(s):  
Adam J. Koppers ◽  
Geoffry N. De Iuliis ◽  
Jane M. Finnie ◽  
Eileen A. McLaughlin ◽  
R. John Aitken
Keyword(s):  
Oxidative Stress ◽  
Reactive Oxygen Species ◽  
Complex I ◽  
Mitochondrial Membrane ◽  
Reactive Oxygen ◽  
Human Spermatozoa ◽  
Ros Generation ◽  
Oxygen Species ◽  
Mitochondrial Ros ◽  
Sperm Movement

Abstract Context: Male infertility has been linked with the excessive generation of reactive oxygen species (ROS) by defective spermatozoa. However, the subcellular origins of this activity are unclear. Objective: The objective of this study was to determine the importance of sperm mitochondria in creating the oxidative stress associated with defective sperm function. Method: Intracellular measurement of mitochondrial ROS generation and lipid peroxidation was performed using the fluorescent probes MitoSOX red and BODIPY C11 in conjunction with flow cytometry. Effects on sperm movement were measured by computer-assisted sperm analysis. Results: Disruption of mitochondrial electron transport flow in human spermatozoa resulted in generation of ROS from complex I (rotenone sensitive) or III (myxothiazol, antimycin A sensitive) via mechanisms that were independent of mitochondrial membrane potential. Activation of ROS generation at complex III led to the rapid release of hydrogen peroxide into the extracellular space, but no detectable peroxidative damage. Conversely, the induction of ROS on the matrix side of the inner mitochondrial membrane at complex I resulted in peroxidative damage to the midpiece and a loss of sperm movement that could be prevented by the concomitant presence of α-tocopherol. Defective human spermatozoa spontaneously generated mitochondrial ROS in a manner that was negatively correlated with motility. Simultaneous measurement of general cellular ROS generation with dihydroethidium indicated that 68% of the variability in such measurements could be explained by differences in mitochondrial ROS production. Conclusion: We conclude that the sperm mitochondria make a significant contribution to the oxidative stress experienced by defective human spermatozoa.

scholarly journals Synthesis of keratine, silver, and flavonols nanocomposites to inhibit oxidative stress in pancreatic beta-cell (INS-1) and reduce intracellular reactive oxygen species production

2021 ◽  
Vol 14 (2) ◽  
pp. 102917
Author(s):  
Rosa Martha Pérez-Gutierrez ◽  
Isis Sherazada Rodríguez-Clavel ◽  
Silvia Patricia Paredes-Carrera ◽  
Jesus Carlos Sánchez-Ochoa ◽  
Alethia Muñiz-Ramirez ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Reactive Oxygen Species ◽  
Beta Cell ◽  
Reactive Oxygen Species Production ◽  
Pancreatic Beta Cell ◽  
Reactive Oxygen ◽  
Intracellular Reactive Oxygen Species ◽  
Oxygen Species ◽  
Species Production
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