Therapeutic Reactive Oxygen Generation

2008 ◽  
Vol 94 (2) ◽  
pp. 278-283 ◽  
Author(s):  
Peter Scharff ◽  
Uwe Ritter ◽  
Olga P Matyshevska ◽  
Svitlana V Prylutska ◽  
Iryna I Grynyuk ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Reactive Oxygen Species ◽  
Reactive Oxygen ◽  
Intracellular Reactive Oxygen Species ◽  
Transformed Cells ◽  
Oxygen Species ◽  
Selective Activation ◽  
Oxygen Generation ◽  
Cell Components ◽  
Nanosilica Particles

An increase of the intracellular reactive oxygen species (ROS) concentration leads to the development of oxidative stress and, thus, to the damage of cell components. The cause-and-effect relations between these processes have not been fully established yet. The ability of photo excited supramolecular composites containing fullerenes C60 immobilized at nanosilica particles to generate reactive oxygen species (ROS) in cells of two types (rat thymocytes, and transformed cells of ascite Erlich carcinoma, EAC, and leucosis L1210) is demonstrated. The damaging effect of photo excited C60-composites are shown, which appeared to be selective and manifested in transformed cells, but not in thymocytes. It has been shown that after the irradiation of aqueous solutions or cell suspensions in the presence of fullerene C60, the generation of reactive oxygen species is observed. It has been shown that the influence of photo excited fullerene C60 on metabolic processes depends on the composition of C60-containing complex and on the type of the cells. The damaging effects of photo excited fullerene C60-containing composites were demonstrated to be selective. The data presented suggest that the application of fullerene C60-containing composites for the selective activation of ROS-dependent death program in certain types of tumor cells is very promising.

scholarly journals Ginkgo biloba extract (EGb 761) attenuates oxidative stress induction in erythrocytes of sickle cell disease patients

2012 ◽  
Vol 48 (4) ◽  
pp. 659-665 ◽  
Author(s):  
Aline Emmer Ferreira Furman ◽  
Railson Henneberg ◽  
Priscila Bacarin Hermann ◽  
Maria Suely Soares Leonart ◽  
Aguinaldo José do Nascimento
Keyword(s):  
Oxidative Stress ◽  
Reactive Oxygen Species ◽  
Lipid Peroxidation ◽  
Sickle Cell ◽  
Reduced Glutathione ◽  
Reactive Oxygen ◽  
Intracellular Reactive Oxygen Species ◽  
Oxygen Species ◽  
Egb 761 ◽  
Cell Disease

Sickle cell disease promotes hemolytic anemia and occlusion of small blood vessels due to the presence of high concentrations of hemoglobin S, resulting in increased production of reactive oxygen species and decreased antioxidant defense capacity. The aim of this study was to evaluate the protective action of a standardized extract of Ginkgo biloba (EGb 761), selected due to its high content of flavonoids and terpenoids, in erythrocytes of patients with sickle cell anemia (HbSS, SS erythrocytes) subjected to oxidative stress using tert-butylhydroperoxide or 2,2-azobis-(amidinepropane)-dihydrochloride, in vitro. Hemolysis indexes, reduced glutathione, methemoglobin concentrations, lipid peroxidation, and intracellular reactive oxygen species were determined. SS erythrocytes displayed increased rates of oxidation of hemoglobin and membrane lipid peroxidation compared to normal erythrocytes (HbAA, AA erythrocytes), and the concentration of EGb 761 necessary to achieve the same antioxidant effect in SS erythrocytes was at least two times higher than in normal ones, inhibiting the formation of intracellular reactive oxygen species (IC50 of 13.6 µg/mL), partially preventing lipid peroxidation (IC50 of 242.5 µg/mL) and preventing hemolysis (IC50 of 10.5 µg/mL). Thus, EGb 761 has a beneficial effect on the oxidative status of SS erythrocytes. Moreover, EGb 761 failed to prevent oxidation of hemoglobin and reduced glutathione at the concentrations examined.

scholarly journals Oxidative Stress in the Newborn Period: Useful Biomarkers in the Clinical Setting

Antioxidants ◽  
2018 ◽  
Vol 7 (12) ◽  
pp. 193 ◽  
Author(s):  
Iván Millán ◽  
José Piñero-Ramos ◽  
Inmaculada Lara ◽  
Anna Parra-Llorca ◽  
Isabel Torres-Cuevas ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Reactive Oxygen Species ◽  
Structural Damage ◽  
Chemical Species ◽  
Birth Asphyxia ◽  
Reactive Oxygen ◽  
Response To Therapy ◽  
Oxygen Species ◽  
Newborn Period ◽  
Cell Components

Aerobic metabolism is highly efficient in providing energy for multicellular organisms. However, even under physiological conditions, an incomplete reduction of oxygen produces reactive oxygen species and, subsequently, oxidative stress. Some of these chemical species are highly reactive free radicals capable of causing functional and structural damage to cell components (protein, lipids, or nucleotides). Oxygen is the most used drug in ill-adapted patients during the newborn period. The use of oxygen may cause oxidative stress-related diseases that increase mortality and cause morbidity with adverse long-term outcomes. Conditions such as prematurity or birth asphyxia are frequently treated with oxygen supplementation. Both pathophysiological situations of hypoxia–reoxygenation in asphyxia and hyperoxia in premature infants cause a burst of reactive oxygen species and oxidative stress. Recently developed analytical assays using mass spectrometry have allowed us to determine highly specific biomarkers with minimal samples. The detection of these metabolites will help improve the diagnosis, evolution, and response to therapy in oxidative stress-related conditions during the newborn period.

scholarly journals Effects of ROS in human adrenocortical carcinoma SW-13 cells

2018 ◽  
Author(s):  
Eriko Shimada ◽  
Yusuke Tsuruwaka
Keyword(s):  
Oxidative Stress ◽  
Reactive Oxygen Species ◽  
Cell Proliferation ◽  
Cell Differentiation ◽  
Adrenocortical Carcinoma ◽  
Reactive Oxygen ◽  
Intracellular Reactive Oxygen Species ◽  
Oxygen Species ◽  
Effective Contact ◽  
Intracellular Ca

Paraquat is a highly effective contact herbicide, an inducer of reactive oxygen species (ROS), which generate oxidative stress in cells. Reactive oxygen species has been known to function as an important messenger in cell differentiation. In this study, we investigated the effect of paraquat on cell differentiation using human adenocortical carcinoma (ACC) SW-13 cells. Paraquat, an inducer of intracellular reactive oxygen species, induced the differentiation of SW-13 cells into neural-like cells in the presence of neurofilament. Moreover, paraquat exposure promoted SW-13 cell proliferation and increased intracellular Ca2+ concentration.

scholarly journals Effects of ROS in human adrenocortical carcinoma SW-13 cells

2018 ◽  
Author(s):  
Eriko Shimada ◽  
Yusuke Tsuruwaka
Keyword(s):  
Oxidative Stress ◽  
Reactive Oxygen Species ◽  
Cell Proliferation ◽  
Cell Differentiation ◽  
Adrenocortical Carcinoma ◽  
Reactive Oxygen ◽  
Intracellular Reactive Oxygen Species ◽  
Oxygen Species ◽  
Effective Contact ◽  
Intracellular Ca

Paraquat is a highly effective contact herbicide, an inducer of reactive oxygen species (ROS), which generate oxidative stress in cells. Reactive oxygen species has been known to function as an important messenger in cell differentiation. In this study, we investigated the effect of paraquat on cell differentiation using human adenocortical carcinoma (ACC) SW-13 cells. Paraquat, an inducer of intracellular reactive oxygen species, induced the differentiation of SW-13 cells into neural-like cells in the presence of neurofilament. Moreover, paraquat exposure promoted SW-13 cell proliferation and increased intracellular Ca2+ concentration.

scholarly journals Synthesis of keratine, silver, and flavonols nanocomposites to inhibit oxidative stress in pancreatic beta-cell (INS-1) and reduce intracellular reactive oxygen species production

2021 ◽  
Vol 14 (2) ◽  
pp. 102917
Author(s):  
Rosa Martha Pérez-Gutierrez ◽  
Isis Sherazada Rodríguez-Clavel ◽  
Silvia Patricia Paredes-Carrera ◽  
Jesus Carlos Sánchez-Ochoa ◽  
Alethia Muñiz-Ramirez ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Reactive Oxygen Species ◽  
Beta Cell ◽  
Reactive Oxygen Species Production ◽  
Pancreatic Beta Cell ◽  
Reactive Oxygen ◽  
Intracellular Reactive Oxygen Species ◽  
Oxygen Species ◽  
Species Production
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