Characterization and regulation of PiDur3, a permease involved in the acquisition of urea by the ectomycorrhizal fungus Paxillus involutus

2008 ◽  
Vol 45 (6) ◽  
pp. 912-921 ◽  
Author(s):  
Mélanie Morel ◽  
Christophe Jacob ◽  
Michael Fitz ◽  
Daniel Wipf ◽  
Michel Chalot ◽  
...  
Keyword(s):  
Ectomycorrhizal Fungus ◽  
Paxillus Involutus

scholarly journals Secretion of Iron(III)-Reducing Metabolites during Protein Acquisition by the Ectomycorrhizal Fungus Paxillus involutus

2020 ◽  
Vol 9 (1) ◽  
pp. 35
Author(s):  
Firoz Shah ◽  
Markus Gressler ◽  
Susan Nehzati ◽  
Michiel Op De Beeck ◽  
Luigi Gentile ◽  
...  
Keyword(s):  
Fenton Reaction ◽  
Ectomycorrhizal Fungus ◽  
Paxillus Involutus ◽  
Environmental Cues ◽  
Rapid Reduction ◽  
Fenton Chemistry ◽  
X Ray ◽  
Solid Iron ◽  
X Ray Absorption ◽  
Step Mechanism

The ectomycorrhizal fungus Paxillus involutus decomposes proteins using a two-step mechanism, including oxidation and proteolysis. Oxidation involves the action of extracellular hydroxyl radicals (•OH) generated by the Fenton reaction. This reaction requires the presence of iron(II). Here, we monitored the speciation of extracellular iron and the secretion of iron(III)-reducing metabolites during the decomposition of proteins by P. involutus. X-ray absorption spectroscopy showed that extracellular iron was mainly present as solid iron(III) phosphates and oxides. Within 1 to 2 days, these compounds were reductively dissolved, and iron(II) complexes were formed, which remained in the medium throughout the incubation. HPLC and mass spectrometry detected five extracellular iron(III)-reducing metabolites. Four of them were also secreted when the fungus grew on a medium containing ammonium as the sole nitrogen source. NMR identified the unique iron(III)-reductant as the diarylcyclopentenone involutin. Involutin was produced from day 2, just before the elevated •OH production, preceding the oxidation of BSA. The other, not yet fully characterized iron(III)-reductants likely participate in the rapid reduction and dissolution of solid iron(III) complexes observed on day one. The production of these metabolites is induced by other environmental cues than for involutin, suggesting that they play a role beyond the Fenton chemistry associated with protein oxidation.

scholarly journals Cadmium-Responsive Thiols in the Ectomycorrhizal Fungus Paxillus involutus

2004 ◽  
Vol 70 (12) ◽  
pp. 7413-7417 ◽  
Author(s):  
Mikael Courbot ◽  
Laurent Diez ◽  
Roberta Ruotolo ◽  
Michel Chalot ◽  
Pierre Leroy
Keyword(s):  
Molecular Mass ◽  
Ectomycorrhizal Fungi ◽  
High Performance ◽  
Metal Tolerance ◽  
Ectomycorrhizal Fungus ◽  
Paxillus Involutus ◽  
Cellular Mechanisms ◽  
Chromatography Method ◽  
Relative Lack ◽  
Liquid Chromatography Method

ABSTRACT Molecular and cellular mechanisms underlying the sustained metal tolerance of ectomycorrhizal fungi are largely unknown. Some of the main mechanisms involved in metal detoxification appear to involve the chelation of metal ions in the cytosol with thiol-containing compounds, such as glutathione, phytochelatins, or metallothioneins. We used an improved high-performance liquid chromatography method for the simultaneous measurement of thiol-containing compounds from cysteine and its derivatives (γ-glutamylcysteine, glutathione) to higher-molecular-mass compounds (phytochelatins). We found that glutathione and γ-glutamylcysteine contents increased when the ectomycorrhizal fungus Paxillus involutus was exposed to cadmium. An additional compound with a 3-kDa molecular mass, most probably related to a metallothionein, increased drastically in mycelia exposed to cadmium. The relative lack of phytochelatins and the presence of a putative metallothionein suggest that ectomycorrhizal fungi may use a different means to tolerate heavy metals, such as Cd, than do their plant hosts.

Molecular cloning, characterization and regulation by cadmium of a superoxide dismutase from the ectomycorrhizal fungus Paxillus involutus

2001 ◽  
Vol 268 (11) ◽  
pp. 3223-3232 ◽  
Author(s):  
Christophe Jacob ◽  
Mikaël Courbot ◽  
Annick Brun ◽  
Howard M. Steinman ◽  
Jean-Pierre Jacquot ◽  
...  
Keyword(s):  
Superoxide Dismutase ◽  
Molecular Cloning ◽  
Ectomycorrhizal Fungus ◽  
Paxillus Involutus

Screening for rapidly evolving genes in the ectomycorrhizal fungus Paxillus involutus using cDNA microarrays

2005 ◽  
Vol 15 (2) ◽  
pp. 535-550 ◽  
Author(s):  
ANTOINE LE QUÉRÉ ◽  
KASPER ASTRUP ERIKSEN ◽  
BALAJI RAJASHEKAR ◽  
ANDRES SCHÜTZENDÜBEL ◽  
BJÖRN CANBÄCK ◽  
...  
Keyword(s):  
Cdna Microarrays ◽  
Ectomycorrhizal Fungus ◽  
Paxillus Involutus

Factors affecting amino acid uptake by the ectomycorrhizal fungus Paxillus involutus

1995 ◽  
Vol 99 (9) ◽  
pp. 1131-1138 ◽  
Author(s):  
M. Chalot ◽  
M.M. Kytöviita ◽  
A. Brun ◽  
R.D. Finlay ◽  
B. Söderström
Keyword(s):  
Amino Acid ◽  
Amino Acid Uptake ◽  
Ectomycorrhizal Fungus ◽  
Paxillus Involutus ◽  
Acid Uptake ◽  
Factors Affecting

Formation de Weddellite (CaC2O4∙2H2O) extracellulaire en culture in vitro par Paxillus involutus; signification de cette production pour la symbiose ecto- mycorhizienne

1984 ◽  
Vol 62 (6) ◽  
pp. 1116-1121 ◽  
Author(s):  
F. Lapeyrie ◽  
Monique Perrin ◽  
R. Pepin ◽  
G. Bruchet
Keyword(s):  
Calcium Oxalate ◽  
Culture Medium ◽  
Calcium Content ◽  
Ectomycorrhizal Fungus ◽  
Paxillus Involutus ◽  
X Ray Diffraction ◽  
X Ray ◽  
High Calcium ◽  
Scanning Electron

Several forms of extracellular crystals have been observed in vitro on a semisynthetic culture medium. The conditions for their formation have not been determined. Their morphology and their localisation on the hyphal surface and in the culture medium have been investigated by means of scanning electron microscopy. These crystals have been identified as dehydrated calcium oxalate (Weddellite) using x-ray diffraction methods. Their high calcium content has been demonstrated with the scanning electron microprobe, by detecting the x-ray emission of calcium. The significance of calcium oxalate production by an ectomycorrhizal fungus is discussed.

Sign in / Sign up

Export Citation Format

Share Document