Optimization of cloned enzyme donor immunoassay cut-offs for drugs of abuse in post-mortem whole blood

2020 ◽  
Vol 312 ◽  
pp. 110291 ◽  
Author(s):  
Guido Pelletti ◽  
Francesca Rossi ◽  
Marco Garagnani ◽  
Rossella Barone ◽  
Raffaella Roffi ◽  
...  
Keyword(s):  
Whole Blood ◽  
Drugs Of Abuse ◽  
Post Mortem

Paper Spray and Extraction Spray Mass Spectrometry for the Direct and Simultaneous Quantification of Eight Drugs of Abuse in Whole Blood

2014 ◽  
Vol 86 (15) ◽  
pp. 7712-7718 ◽  
Author(s):  
Ryan D. Espy ◽  
Sebastiaan Frans Teunissen ◽  
Nicholas E. Manicke ◽  
Yue Ren ◽  
Zheng Ouyang ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Whole Blood ◽  
Drugs Of Abuse ◽  
Paper Spray ◽  
Simultaneous Quantification

A COMPARISON OF THE ROUTINE RAPID WHOLE BLOOD (STAINED ANTIGEN) AND THE ROUTINE RAPID SERUM AGGLUTINATION TESTS FOR PULLORUM DISEASE

1934 ◽  
Vol 10 (6) ◽  
pp. 798-806
Author(s):  
Jacob Biely ◽  
W. Roach
Keyword(s):  
Whole Blood ◽  
Agglutination Test ◽  
Test Laboratory ◽  
Post Mortem ◽  
Post Mortem Examination ◽  
Experiment Station ◽  
Commercial Laboratory ◽  
Serum Agglutination

Data are presented on 4,429 birds, comprising eight flocks, which were tested for pullorum disease by the whole blood agglutination test and the rapid serum agglutination test (commercial laboratory). The diagnoses agreed in the cases of 4,046 birds (97.24%) and disagreed in the cases of 122 birds (2.75%).Of the 122 birds, 43 were diagnosed as positive by the whole blood agglutination test and as negative by the rapid serum agglutination test, while 79 were diagnosed as positive by the rapid serum agglutination test and as negative by the whole blood agglutination test.Of the 122 birds, 102 were retested by the whole blood, rapid serum (Laboratory 1), and rapid serum agglutination test (Laboratory 2, (Experiment Station Laboratory)).There was a closer agreement between the diagnoses made on the basis of the whole blood and rapid serum tests (Laboratory 2) than between those made on the basis of the rapid serum (Laboratory 1) and rapid serum (Laboratory 2) tests (71.56% and 62.37% respectively).A detailed study of the retests and post-mortem examination of the 102 birds is presented.

scholarly journals Forensic NMR metabolomics: one more arrow in the quiver

Metabolomics ◽  
2020 ◽  
Vol 16 (11) ◽  
Author(s):  
Emanuela Locci ◽  
Giovanni Bazzano ◽  
Alberto Chighine ◽  
Francesco Locco ◽  
Ernesto Ferraro ◽  
...  
Keyword(s):  
Drugs Of Abuse ◽  
Recent Literature ◽  
Metabolic Profiles ◽  
Time Since Death ◽  
Post Mortem ◽  
Nmr Metabolomics ◽  
Additional Information ◽  
Shed Light ◽  
Metabolic Signatures

Abstract Introduction NMR metabolomics is increasingly used in forensics, due to the possibility of investigating both endogenous metabolic profiles and exogenous molecules that may help to describe metabolic patterns and their modifications associated to specific conditions of forensic interest. Objectives The aim of this work was to review the recent literature and depict the information provided by NMR metabolomics. Attention has been devoted to the identification of peculiar metabolic signatures and specific ante-mortem and post-mortem profiles or biomarkers related to different conditions of forensic concern, such as the identification of biological traces, the estimation of the time since death, and the exposure to drugs of abuse. Results and Conclusion The results of the described studies highlight how forensics can benefit from NMR metabolomics by gaining additional information that may help to shed light in several forensic issues that still deserve to be further elucidated.

scholarly journals Ethanol Determination in Post-Mortem Samples: Correlation between Blood and Vitreous Humor Concentration

Molecules ◽  
2020 ◽  
Vol 25 (12) ◽  
pp. 2724
Author(s):  
Fabio Savini ◽  
Angela Tartaglia ◽  
Ludovica Coccia ◽  
Danilo Palestini ◽  
Cristian D’Ovidio ◽  
...  
Keyword(s):  
Whole Blood ◽  
Dynamic Range ◽  
Limit Of Detection ◽  
Linear Regression Analysis ◽  
Vitreous Humor ◽  
Alcohol Concentration ◽  
Coefficient Of Determination ◽  
Post Mortem ◽  
Excessive Alcohol Consumption ◽  
Ethanol Determination

Ethanol (ethylic alcohol) represents the most commonly used drug worldwide and is often involved in clinical and forensic toxicology. Based on several reports, excessive alcohol consumption is the main contributing factor in traffic accidents, drownings, suicides, and other crimes. For these reasons, it becomes essential to analyze the alcohol concentration during autopsy. Although blood is usually used for alcohol analysis in post-mortem cases, it could suffer alterations, putrefaction, and microbial contaminations. As an alternative to whole blood, vitreous humor has been successfully used in medico-legal studies. In this work, post-mortem specimens were analyzed for ethanol determination. The analysis of blood and vitreous humor were carried-out using gas chromatography-flame ionized detector (GC-FID) with a total run time of 6 min. The method was validated in terms of limit of detection, limit of quantification, dynamic range, sensibility, recovery, precision and trueness. A linear regression analysis indicated a coefficient of determination (R2) of 0.9981. The study confirmed no statistically differences between alcohol concentration in blood and vitreous humor, leading vitreous humor as an excellent matrix that could be used as an alternative to whole blood in toxicological analysis in cases where blood is not available.

scholarly journals Enterocolitis Caused by Ehrlichia sp. in the Horse (Potomac Horse Fever)

1986 ◽  
Vol 23 (4) ◽  
pp. 471-477 ◽  
Author(s):  
D. O. Cordes ◽  
B. D. Perry ◽  
Y. Rikihisa ◽  
W. R. Chickering
Keyword(s):  
Mast Cells ◽  
Epithelial Cells ◽  
Acute Phase ◽  
Whole Blood ◽  
Clinical Signs ◽  
Post Mortem ◽  
Potomac Horse Fever ◽  
Incubation Periods ◽  
Mucous Membranes ◽  
Post Mortem Findings

Potomac horse fever was reproduced in 15 ponies by transfusion of whole blood originally from two natural cases and subsequently from ponies infected by the transfusions. Incubation periods varied from 9 to 15 days. Affected ponies developed varying degrees of fever, diarrhea, anorexia, depression, and leukopenia. Eleven affected ponies were killed, three died in the acute phase of the disease, and one did not show clinical signs. The most consistent post-mortem findings were fluid contents in the cecum and large colon, and areas of hyperemia (of inconstant degree and distribution) in mucosae of both small and large intestines. Multifocal areas of necrosis occurred in mucous membranes. Ehrlichial organisms were most common in the cytoplasm of epithelial cells, macrophages, and mast cells of the large colon.

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