Extracellular vesicles piwi-interacting RNAs from skin mucus for identification of infected Cynoglossus semilaevis with Vibrio harveyi

Vibrio harveyi causes vibriosis in various commercial marine fish species. The infection leads to significant economic losses for aquaculture farms, and vaccination is an alternative approach for the prevention and control of fish diseases for aquaculture sustainability. This study describes the use of formalin-killed Vibrio harveyi (FKVh) strain Vh1 as a vaccine candidate to stimulate innate and adaptive immunities against vibriosis in a marine red hybrid tilapia model. Tilapia are fast growing; cheap; resistant to diseases; and tolerant to adverse environmental conditions of fresh water, brackish water, and marine water and because of these advantages, marine red hybrid tilapia is a suitable candidate as a model to study fish diseases and vaccinations against vibriosis. A total of 180 healthy red hybrid tilapias were gradually adapted to the marine environment before being divided into two groups, with 90 fish in each group and were kept in triplicate with 30 fish per tank. Group 1 was vaccinated intraperitoneally with 100 µL of FKVh on week 0, and a booster dose was similarly administered on week 2. Group 2 was similarly injected with PBS. Skin mucus, serum, and gut lavage were collected weekly for enzyme-linked immunosorbent assay (ELISA) and a lysozyme activity assay from a total of 30 fish of each group. On week 4, the remaining 60 fish of Groups 1 and 2 were challenged with 108 cfu/fish of live Vibrio harveyi. The clinical signs were monitored while the survival rate was recorded for 48 h post-challenge. Vaccination with FKVh resulted in a significantly (p < 0.05) higher rate of survival (87%) compared to the control (20%). The IgM antibody titer and lysozyme activities of Group 1 were significantly (p < 0.05) higher than the unvaccinated Groups 2 in most weeks throughout the experiment. Therefore, the intraperitoneal exposure of marine red hybrid tilapia to killed V. harveyi enhanced the resistance and antibody response of the fish against vibriosis.

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Genetic analysis of disease resistance to Vibrio harveyi by challenge test in Chinese tongue sole (Cynoglossus semilaevis)

Aquaculture ◽

10.1016/j.aquaculture.2019.01.011 ◽

2019 ◽

Vol 503 ◽

pp. 430-435 ◽

Cited By ~ 7

Author(s):

Yangzhen Li ◽

Lei Wang ◽

Yingming Yang ◽

Xue Li ◽

Huan Dai ◽

...

Keyword(s):

Disease Resistance ◽

Genetic Analysis ◽

Vibrio Harveyi ◽

Challenge Test ◽

Cynoglossus Semilaevis ◽

Tongue Sole

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Comparative transcriptome profiling of immune response against Vibrio harveyi infection in Chinese tongue sole

Scientific Data ◽

10.1038/s41597-019-0231-2 ◽

2019 ◽

Vol 6 (1) ◽

Cited By ~ 2

Author(s):

Hao Xu ◽

Xiwen Xu ◽

Xihong Li ◽

Lei Wang ◽

Jiayu Cheng ◽

...

Keyword(s):

Immune Response ◽

Molecular Mechanisms ◽

Vibrio Harveyi ◽

Bacterial Pathogen ◽

Transcriptome Profiling ◽

Transcript Level ◽

Cynoglossus Semilaevis ◽

Illumina Hiseq ◽

Tongue Sole ◽

And Control

Abstract Vibrio harveyi is a major bacterial pathogen that causes fatal vibriosis in Chinese tongue sole (Cynoglossus semilaevis), resulting in massive mortality in the farming industry. However, the molecular mechanisms of C. semilaevis response to V. harveyi infection are poorly understood. Here, we performed transcriptomic analysis of C. semilaevis, comparing resistant and susceptible families in response to V. harveyi challenge (CsRC and CsSC) and control conditions (CsRU and CsSU). RNA libraries were constructed using 12 RNA samples isolated from three biological replicates of the four groups. We performed transcriptome sequencing on an Illumina HiSeq platform, and generating a total of 1,095 million paired-end reads, with the number of clean reads per library ranging from 75.27 M to 99.97 M. Through pairwise comparisons among the four groups, we identified 713 genes exhibiting significant differences at the transcript level. Furthermore, the expression levels were validated by real-time qPCR. Our results provide a valuable resource and new insights into the immune response to V. harveyi infection.

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Proteomics of mucosal exosomes of Cynoglossus semilaevis altered when infected by Vibrio harveyi

Developmental & Comparative Immunology ◽

10.1016/j.dci.2021.104045 ◽

2021 ◽

pp. 104045

Author(s):

Na Zhao ◽

Lei Jia ◽

Xiaoxu He ◽

Bo Zhang

Keyword(s):

Vibrio Harveyi ◽

Cynoglossus Semilaevis

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Development of a 38K Single Nucleotide Polymorphism Array and Application in Henomic Selection for Resistance Against Vibrio harveyi in Chinese Tongue sole, Cynoglossus Semilaevis

10.21203/rs.3.rs-48867/v1 ◽

2020 ◽

Author(s):

Sheng Lu ◽

Qian Zhou ◽

Yadong Chen ◽

Yang Liu ◽

Yangzhen Li ◽

...

Keyword(s):

Single Nucleotide Polymorphism ◽

Vibrio Harveyi ◽

Reference Group ◽

Single Nucleotide Polymorphism Array ◽

Snp Array ◽

Cynoglossus Semilaevis ◽

Breeding Value ◽

Nucleotide Polymorphism ◽

Single Nucleotide ◽

Tongue Sole

Abstract Background: In recent years, the disease outbreak caused by Vibrio harveyi upset the booming development of the Chinese tongue sole (Cynoglossus semilaevis) farming industry. Genomic selection (GS) is a powerful method to improve the traits of interest, which has been proved in livestock and some fishes. Besides, the single nucleotide polymorphism (SNP) array is an efficient genotyping platform that can be used for genetic studies. To improve V. harveyi resistance in C. semilaevis, we firstly constructed a reference group of 1,572 individuals and investigated accuracies of four genomic methods (genomic best linear unbiased prediction (GBLUP), weighted GBLUP, BayesB, and BayesC) at predicting the genomic estimated breeding value (GEBV) using five-fold cross-validation and SNPs varying from 0.5 k to 500 k. Then, an SNP array was developed using the Affymetrix Axiom technology, and its accuracy in genotyping was evaluated by comparing SNPs generated by the array and by the re-sequencing technology. Finally, we selected 44 candidates as the parents of 23 families of C. semilaevis to evaluate the feasibility of the SNP array for GS.Results: all genomic methods outperformed the pedigree-based BLUP (ABLUP) when at least 50 k SNPs used for prediction, of which GBLUP resulted in better estimation than ABLUP when more than 1 k SNPs used. A 38 k SNP array, “Solechip No.1”, was developed with an average of 10.5 kb inter-spacing between two adjacent SNPs. The SNPs generated by the array and by the re-sequencing reached an average consistency of 94.8 %, of which 79.3 % of loci had a more than 90 % of the consistency. The survival rates of these 23 offspring families had a correlation of 0.706 with the family GEBVs (mid-parental GEBVs), and the average survival rate of the top five families in GEBVs (79.1 %) is higher than the bottom five families (58.1 %).Conclusion: GS is an efficient method to improve the V. harveyi resistance in C. semilaevis, and the SNP array “Solechip No.1” is a convenient and reliable tool for the Chinses tongue sole selective breeding practice.

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