scholarly journals Author Contributions:Anne Bakke: Conceptualization, Methodology, Writing – original draft preparation, Writing – review & editing; Alexander Rebl: Conceptualization, Methodology; Petter Frost: Writing – review & editing; Sergey Afanasyev: Software and Data curation; Kristoffer Alnes Røyset: Methodology, Writing – review & editing; Tina Søfteland: Writing – review & editing; Hege Lund: Conceptualization, Writing – review & editing, Supervision; Preben Boysen: Conceptualization, Writing – review & editing, Supervision, Project administration and Funding acquisition; Aleksei Krasnov: Conceptualization, Methodology, Writing – original draft preparation, Writing – review & editing, Project administration and Funding acquisition, All authors have read and agreed to the published version of the manuscript.Effect of two constant light regimens on antibody profiles and immune gene expression in atlantic salmon following vaccination and experimental challenge with salmonid alphavirus

Author(s):  
Anne Bakke ◽  
Alexander Rebl ◽  
Petter Frost ◽  
Sergey Afanasyev ◽  
Kristoffer Alnes Røyset ◽  
...  
2015 ◽  
Vol 42 (2) ◽  
pp. 522-529 ◽  
Author(s):  
Victoria A. Valdenegro-Vega ◽  
Mark Polinski ◽  
Andrew Bridle ◽  
Philip Crosbie ◽  
Melanie Leef ◽  
...  

2018 ◽  
Vol 8 (1) ◽  
Author(s):  
Shiferaw Jenberie ◽  
Hanna L. Thim ◽  
J. Oriol Sunyer ◽  
Karsten Skjødt ◽  
Ingvill Jensen ◽  
...  

2018 ◽  
Vol 8 (1) ◽  
Author(s):  
Shiferaw Jenberie ◽  
Hanna L. Thim ◽  
J. Oriol Sunyer ◽  
Karsten Skjødt ◽  
Ingvill Jensen ◽  
...  

2020 ◽  
Vol 8 (10) ◽  
pp. 1609
Author(s):  
Natacha Santibañez ◽  
Matías Vega ◽  
Tatiana Pérez ◽  
Alejandro Yáñez ◽  
Roxana González-Stegmaier ◽  
...  

Piscirickettsia salmonis is the causative agent of Piscirickettsiosis, an infectious disease with a high economic impact on the Chilean salmonid aquaculture industry. This bacterium produces biofilm as a potential resistance and persistence strategy against stressful environmental stimuli. However, the in vitro culture conditions that modulate biofilm formation as well as the effect of sessile bacteria on virulence and immune gene expression in host cells have not been described for P. salmonis. Therefore, this study aimed to analyze the biofilm formation by P. salmonis isolates under several NaCl and iron concentrations and to evaluate the virulence of planktonic and sessile bacteria, together with the immune gene expression induced by these bacterial conditions in an Atlantic salmon macrophage cell line. Our results showed that NaCl and Fe significantly increased biofilm production in the LF-89 type strain and EM-90-like isolates. Additionally, the planktonic EM-90 isolate and sessile LF-89 generated the highest virulence levels, associated with differential expression of il-1β, il-8, nf-κb, and iκb-α genes in SHK-1 cells. These results suggest that there is no single virulence pattern or gene expression profile induced by the planktonic or sessile condition of P. salmonis, which are dependent on each strain and bacterial condition used.


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