Evolutionary fate of duplicate genes encoding aspartic proteinases. Nothepsin case study

Gene ◽  
2006 ◽  
Vol 368 ◽  
pp. 101-109 ◽  
Author(s):  
Lucia Borrelli ◽  
Roberta De Stasio ◽  
Silvana Filosa ◽  
Elio Parisi ◽  
Marilisa Riggio ◽  
...  
Keyword(s):  
Duplicate Genes ◽  
Aspartic Proteinases ◽  
Genes Encoding ◽  
Evolutionary Fate

Dietary L-leucine improves the anemia in a mouse model for Diamond-Blackfan anemia

Blood ◽  
2012 ◽  
Vol 120 (11) ◽  
pp. 2225-2228 ◽  
Author(s):  
Pekka Jaako ◽  
Shubhranshu Debnath ◽  
Karin Olsson ◽  
David Bryder ◽  
Johan Flygare ◽  
...  
Keyword(s):  
Mouse Model ◽  
Ribosomal Proteins ◽  
Therapeutic Agent ◽  
Hemoglobin Concentration ◽  
Response To Treatment ◽  
Diamond Blackfan Anemia ◽  
Genes Encoding ◽  
Marrow Cellularity ◽  
Deficient Mice

Abstract Diamond-Blackfan anemia (DBA) is a congenital erythroid hypoplasia caused by a functional haploinsufficiency of genes encoding for ribosomal proteins. Recently, a case study reported a patient who became transfusion-independent in response to treatment with the amino acid L-leucine. Therefore, we have validated the therapeutic effect of L-leucine using our recently generated mouse model for RPS19-deficient DBA. Administration of L-leucine significantly improved the anemia in Rps19-deficient mice (19% improvement in hemoglobin concentration; 18% increase in the number of erythrocytes), increased the bone marrow cellularity, and alleviated stress hematopoiesis. Furthermore, the therapeutic response to L-leucine appeared specific for Rps19-deficient hematopoiesis and was associated with down-regulation of p53 activity. Our study supports the rationale for clinical trials of L-leucine as a therapeutic agent for DBA.

Selection of antigens for use in a virus-vectored immunocontraceptive vaccine: PH-20 as a case study

1997 ◽  
Vol 9 (1) ◽  
pp. 117 ◽  
Author(s):  
Michael K. Holland ◽  
Jason Andrews ◽  
Hannah Clarke ◽  
Clayton Walton ◽  
Lyn A. Hinds
Keyword(s):  
Oryctolagus Cuniculus ◽  
Genetically Engineered ◽  
Myxoma Virus ◽  
Wild Rabbit ◽  
Pest Species ◽  
Genes Encoding ◽  
Overall Efficiency ◽  
Selection Of ◽  
Sperm Antigen

The European wild rabbit (Oryctolagus cuniculus) has become the major agricultural and environmental pest species in Australia. Current methods of rabbit control are lethal procedures which are increasingly questioned for their overall efficiency, applicability, specificity, cost and humaneness. New initiatives are required. One such initiative is virus-vectored immunocontraception. In this approach, the lagomorph-specific myxoma virus will be genetically engineered to include genes encoding components of rabbit gametes which can induce an immune response that causes infertility. Central to such a strategy is the ability to identify antigens capable of inducing an immunocontraceptive response. A strategy for identifying such antigens has been described previously. A case study of one sperm antigen, PH-20, is reported here. The issues involved in developing this antigen to the stage where it could be considered as a candidate for insertion into a recombinant myxoma virus with the ultimate goal of testing for immunocontraceptive efficacy are discussed. Techniques for inserting genes into myxoma virus have been described previously. The knowledge gained from research with this particular antigen are broadly applicable to other antigens used for both immunocontraceptive vaccines in general and, specifically, for virus-vectored immunocontraception.

Multiplicity of genes encoding secreted aspartic proteinases inCandidaspecies

1994 ◽  
Vol 13 (2) ◽  
pp. 357-368 ◽  
Author(s):  
Michel Monod ◽  
Giuseppe Togni ◽  
Bernhard Hube ◽  
Dominique Sanglard
Keyword(s):  
Aspartic Proteinases ◽  
Genes Encoding

scholarly journals Association of Toll –like Receptors 1, 2, 4, 6 ,8, 9 and 10 Genes Polymorphisms and Susceptibility to Tuberculosis in Sudanese Patients

2021 ◽  
Author(s):  
Najwa A Mhmoud
Keyword(s):  
Complex Disease ◽  
Toll Like Receptors ◽  
Tuberculosis Patients ◽  
Wide Range ◽  
Pcr Rflp ◽  
Genes Encoding ◽  
Study Population ◽  
Polymerase Chain ◽  
Tuberculosis Disease

Abstract Background: Genetic factors are important contributors to the development of a wide range of complex disease. Polymorphisms in genes encoding for toll like receptors (TLRs) usually influence the efficiency of the immune response to infection and are associated with disease susceptibility and progression. Therefore, we aims to describe the first association between TLR1, TLR2, TLR4 TLR6 , TLR8 , TLR9 ,and TLR10 genes polymorphisms and susceptibility to tuberculosis in Sudanese patients.Methodology: Here we performed a case study which included 160 tuberculosis patients and 220 healthy matched controls from Sudan. In the study population, we evaluated the possible association between 86 markers in TLR1, TLR2, TLR4 TLR6 , TLR8 , TLR9 ,and TLR10 genes polymorphisms and susceptibility to tuberculosis disease in Sudanese population using polymerase chain reaction and restriction fragment length polymorphism (PCR-RFLP) . Result: From our results It appeared that in the tuberculosis population the TLR1 rs5743557, TLR1 rs4833095, TLR1 rs5743596, TLR2 rs5743704, TLR2 rs5743708, TLR2 rs3804099 , TLR4 rs4986790 ,TLR4 rs4986791, TLR6 rs5743810 , TLR8 rs3764879 , TLR8 rs3764880, TLR9 rs352165, TLR9 rs352167, TLR9 rs187084 and TLR10 rs4129009 were significantly more often encountered (p<0.0001) than in the control population and were associated with tuberculosis in the Sudanese population. For the other polymorphisms tested, no association with tuberculosis was found in the population tested. Conclusion: This indicates that the genotypes obtained for TLR1 rs5743557, TLR1 rs4833095, TLR1 rs5743596, TLR2 rs5743704, TLR2 rs5743708, TLR2 rs3804099 , TLR4 rs4986790 ,TLR4 rs4986791, TLR6 rs5743810 , TLR8 rs3764879 , TLR8 rs3764880, TLR9 rs352165, TLR9 rs352167, TLR9 rs187084 and TLR10 rs4129009 allele have a significant role in the genetic susceptibility to development tuberculosis in Sudanese population.

scholarly journals Asymmetric evolution of duplicate genes encoding the CCAAT-binding factor NF-Y in plant genomes

2004 ◽  
Vol 165 (2) ◽  
pp. 623-632 ◽  
Author(s):  
Ji Yang ◽  
Zengyan Xie ◽  
Beverley J. Glover
Keyword(s):  
Duplicate Genes ◽  
Plant Genomes ◽  
Binding Factor ◽  
Genes Encoding

The Evolutionary Fate and Consequences of Duplicate Genes

Science ◽  
2000 ◽  
Vol 290 (5494) ◽  
pp. 1151-1155 ◽  
Author(s):  
M. Lynch
Keyword(s):  
Duplicate Genes ◽  
Evolutionary Fate

Presence of duplicate genes encoding a phylogenetically new subgroup of form I ribulose 1,5-bisphosphate carboxylase/oxygenase in Mycobacterium sp. strain JC1 DSM 3803

2009 ◽  
Vol 160 (2) ◽  
pp. 159-165 ◽  
Author(s):  
Sae Woong Park ◽  
Eun Ha Hwang ◽  
Hak Sun Jang ◽  
Jae Ho Lee ◽  
Beom Sik Kang ◽  
...  
Keyword(s):  
Duplicate Genes ◽  
Bisphosphate Carboxylase ◽  
Genes Encoding

Two aspartic proteinases secreted by the pathogenic yeast Candida parapsilosis differ in expression pattern and catalytic properties

2009 ◽  
Vol 390 (3) ◽  
Author(s):  
Olga Hrušková-Heidingsfeldová ◽  
Jiří Dostál ◽  
Filip Majer ◽  
Jana Havlíková ◽  
Martin Hradilek ◽  
...  
Keyword(s):  
Substrate Specificity ◽  
Nitrogen Source ◽  
Candida Parapsilosis ◽  
Nitrogen Sources ◽  
Aspartic Proteinases ◽  
Pathogenic Yeast ◽  
Exogenous Protein ◽  
Genes Encoding ◽  
Order Of Magnitude

Abstract Secreted aspartic proteinases (Sap) play a role in the virulence of pathogenic Candida spp. Candida parapsilosis possesses three genes encoding these enzymes: SAPP1, SAPP2, and SAPP3. We analyzed the expression of the SAPP1 and SAPP2 genes and the production of Sapp1p and Sapp2p proteinases in the presence of different nitrogen sources. While the SAPP2 transcript was present under all of the conditions tested, expression of SAPP1 was induced only by the presence of exogenous protein as the sole nitrogen source. The concentration of Sapp1p in the medium upon induction was at least one order of magnitude higher than the concentration of Sapp2p in all media tested in this study. Enzymological characterization of purified Sapp1p and Sapp2p demonstrated that Sapp2p has a more restricted substrate specificity and significantly lower catalytic activity than Sapp1p. Homology models of Sapp1p and Sapp2p revealed structural motifs that may be responsible for the differences between these two enzymes. Our results indicate that C. parapsilosis secretes a low level of Sapp2p proteinase with narrow substrate specificity and low proteolytic activity under most conditions, while expression and secretion of a higher amount of catalytically efficient Sapp1p enzymes is triggered in the presence of exogenous protein serving as a nitrogen source.

The Genesis and Internal Dynamics of El Salvador's People's Revolutionary Army, 1970–1976

2014 ◽  
Vol 38 (01) ◽  
pp. 102-129
Author(s):  
ALBERTO MARTÍN ÁLVAREZ ◽  
EUDALD CORTINA ORERO
Keyword(s):  
Latin America ◽  
El Salvador ◽  
Early Years ◽  
Left Wing ◽  
Internal Dynamics ◽  
Internal Coherence ◽  
The 1960S

AbstractUsing interviews with former militants and previously unpublished documents, this article traces the genesis and internal dynamics of the Ejército Revolucionario del Pueblo (People's Revolutionary Army, ERP) in El Salvador during the early years of its existence (1970–6). This period was marked by the inability of the ERP to maintain internal coherence or any consensus on revolutionary strategy, which led to a series of splits and internal fights over control of the organisation. The evidence marshalled in this case study sheds new light on the origins of the armed Salvadorean Left and thus contributes to a wider understanding of the processes of formation and internal dynamics of armed left-wing groups that emerged from the 1960s onwards in Latin America.

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