Analysis of hepatic transcriptome modulation exerted by γ-conglutin from lupins in a streptozotocin-induced diabetes model

Gene ◽  
2020 ◽  
Vol 761 ◽  
pp. 145036 ◽  
Author(s):  
Tereso Jovany Guzmán ◽  
Belinda Vargas-Guerrero ◽  
Pedro Macedonio García-López ◽  
Carmen Magdalena Gurrola-Díaz
Keyword(s):  
Diabetes Model ◽  
Streptozotocin Induced Diabetes ◽  
Hepatic Transcriptome

Elevation of Soluble Thrombomodulin Antigen Levels in the Serum and Urine of Streptozotocin-Induced Diabetes Model Rats

2000 ◽  
Vol 99 (1) ◽  
pp. 83-91 ◽  
Author(s):  
Masahiko Nakano ◽  
Masayuki Furutani ◽  
Hiroyuki Shinno ◽  
Tomohiro Ikeda ◽  
Koji Oida ◽  
...  
Keyword(s):  
Soluble Thrombomodulin ◽  
Diabetes Model ◽  
Streptozotocin Induced Diabetes ◽  
Serum And Urine

scholarly journals Keratinocytes differentiation and wound healing in rats with streptozotocin – induced diabetes and severe hyperglycemia

2020 ◽  
Vol 23 (1) ◽  
pp. 19-28 ◽  
Author(s):  
Evgeniy V. Ivanov ◽  
Maria P. Morozova ◽  
Ekaterina M. Rzhavina ◽  
Anna M. Gorbacheva ◽  
Svetlana A. Gavrilova ◽  
...  
Keyword(s):  
Diabetes Mellitus ◽  
Wound Healing ◽  
Its Region ◽  
Repair Process ◽  
Diabetic Rat ◽  
Control Group ◽  
Intact Skin ◽  
Skin Repair ◽  
Diabetes Model ◽  
Streptozotocin Induced Diabetes

BACKGROUND: Diabetes mellitus leads to disruption of the skin repair processes, but the leading mechanisms of this pathology have not yet been identified. In this regard, in our work, we decided to check how hyperglycaemia affects the process of keratinocyte phenotype changes during wound healing. AIMS: To study the effect of hyperglycaemia on wound healing and differentiation of keratinocytes in a rat streptozotocin-induced diabetes model. MATERIALS AND METHODS: Diabetes mellitus was induced in rats by using streptozotocin, 65 mg / kg, intraperitoneally, once. The wound was applied in the supra-scapular region on the 42nd day, after which (after 8, 16, and 24 days) the repair process was evaluated using histological methods. Immunohistochemistry was used to evaluate the expression of cytokeratin-10 and cytokeratin-17. RESULTS: In rats with diabetes mellitus, wound healing slowed down in the later stages, compared with the control group. In general, wound healing was accompanied by an increase in the expression of cytokeratin-10 in its region compared with intact skin, and contractile keratinocytes activation was disrupted in diabetic rat wounds. CONCLUSIONS: Hyperglycaemia slightly slows wound healing in rats and impairs contractile keratinocytes activation.

scholarly journals The Synergistic Effects of Ixeris dentata and Lactobacillus gasseri Extracts on a Diabetes-induced Dry Mouth Model by Enhancing Antioxidation

2020 ◽  
Author(s):  
Hwa Young Lee ◽  
Mingkun Gu ◽  
Jinhua Cheng ◽  
Joo Won Suh ◽  
Han-Jung Chae
Keyword(s):  
Synergistic Effects ◽  
Salivary Flow ◽  
Diabetic Rats ◽  
Dry Mouth ◽  
Oxidative Enzymes ◽  
Diabetic Patients ◽  
Aquaporin 5 ◽  
Diabetes Model ◽  
Streptozotocin Induced Diabetes ◽  
Ixeris Dentata

Dry mouth, hyposalivation, or xerostomia is a significant problem in diabetic patients; however, there was no way to relieve these symptoms. This study was aimed to evaluate the effects of Ixeris dentata (IXD) in combination with lactobacillus extract on the salivation rate in diabetes-induced dry mouth, and its mechanism was also investigated. In the streptozotocin-induced diabetes model, dry mouth condition was established as a model. Both control and diabetic rats were treated with a sublingual spray of either water or IXD and subsequently treated with or without a spray of lactobacillus extract. In diabetes condition, the salivary flow rate, amylase activity, and aquaporin-5 and Na+/H+ exchanger (NHE-1) expressions were markedly decreased, whereas they were more significantly recovered in the sequential treatment of IXD-lactobacillus extract than each single treatment. Furthermore, oxidative stress and its related ER stress response were especially regulated in the IXD/lactobacillus extract condition, where the following anti-oxidative enzymes; GSH:GSSG ratio, superoxide dismutase (SOD), and glutathione peroxidase (GPx) were involved. This study suggests that the combination of IXD and lactobacillus would be a potential alternative medicine against diabetes-induced hyposalivation and xerostomia.

Effect of standard and high-fat diets on the development of complications in a streptozotocin-induced diabetes model in rats

2020 ◽  
Vol 170 (12) ◽  
pp. 720-724
Author(s):  
E. R. Shaykhutdinova ◽  
◽  
V. A. Palikov ◽  
Y. A. Palikova ◽  
N. A. Borozdina ◽  
...  
Keyword(s):  
High Fat ◽  
Diabetes Model ◽  
High Fat Diets ◽  
Streptozotocin Induced Diabetes ◽  
Fat Diets

scholarly journals Experimental evidences of antidiabetic activity of aqueous extract of Cressa cretica L. on streptozotocin induced diabetes in rats

2020 ◽  
Vol 9 (1) ◽  
pp. 774-778
Keyword(s):  
Body Weight ◽  
Aqueous Extract ◽  
Health Care Systems ◽  
Antidiabetic Activity ◽  
Whole Plant ◽  
Diabetes Model ◽  
Care Systems ◽  
Streptozotocin Induced Diabetes ◽  
The Rich ◽  
Histopathological Studies

With the emerging worldwide interest in adopting and studying traditional systems of medication and exploiting their potential based on different health care systems, the evaluation of the rich heritage of traditional medicine is essential. From the innumerable plants being researched since time immemorial, Cressa cretica is an important one. The present study discusses the antidiabetic activity of the entire plant of Cressa cretica L. belonging to Convolvulaceae family.From the thorough investigation of the available literature, it was evident that the plant drug possessed traditional anti-diabetic activity. The aqueous extract of the whole plant of Cressa cretica was subjected to streptozotocin induced diabetes model. The physico-metabolic parameters measured were body weight, Triglycerides, Cholesterol, LDL, HDL, and histopathological studies of pancreas were also observed. The obtained results indicate the anti-diabetic activity of Cressa cretica whole plant extract (200 mg/kg and 400 mg/kg) were increase in body weight, HDL level and reductions in levels of TG, TCH, LDL respectively

scholarly journals A Simplified Streptozotocin-Induced Diabetes Model in Nude Mice

Acta Naturae ◽  
2020 ◽  
Vol 12 (4) ◽  
pp. 98-104
Author(s):  
I. G. Gvazava ◽  
A. V. Kosykh ◽  
O. S. Rogovaya ◽  
O. P. Popova ◽  
K. A. Sobyanin ◽  
...  
Keyword(s):  
Construct Validity ◽  
Nude Mice ◽  
Diabetes Remission ◽  
Face Validity ◽  
High Dose ◽  
Preclinical Studies ◽  
Plasma Insulin Concentration ◽  
Nude Mouse Model ◽  
Diabetes Model ◽  
Streptozotocin Induced Diabetes

Preclinical studies of human cellular and tissue-based products (HCT/Ps) for transplantation therapy of type 1 diabetes mellitus (T1DM) necessarily involve animal models, particularly mouse models of diabetes induced by streptozotocin (STZ). These models should mimic the clinical and metabolic manifestations of T1DM in humans (face validity) and be similar to T1DM in terms of the pathogenetic mechanism (construct validity). Furthermore, since HCT/Ps contain human cells, modeling of diabetes in immune-deficient animals is obligatory. Here we describe the most simplified diabetes model in Nude mice. Diabetes was induced in 31 males by a single intraperitoneal injection of STZ in normal saline at a medium-to-high dose of 150 mg/kg body weight. Fourteen control animals received only saline. Non-fasting plasma glucose (PG) levels were measured periodically for 50 days. All STZ-treated mice survived beyond 50 days. By day 15 after STZ administration, 22 of 31 (71%) mice developed stable diabetes based on the following criteria: (1) non-fasting PG 15 mmol/L on consecutive measurements up until day 50; (2) no diabetes remission. The mean non-fasting PG in mice with stable diabetes over the period of 35 days was equal to 25.7 mmol/L. On day 50, mean plasma insulin concentration, mean pancreatic insulin content, and the average number of -cells in pancreatic islets were 2.6, 8.4, and 50 times lower, respectively, than in the control animals. We consider that our Nude mouse model of diabetes meets face validity and construct validity criteria and can be used in preclinical studies of HCT/Ps.

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