Presence of virulence factor genes (gelE and esp) and biofilm formation in clinical Enterococcus faecalis and Enterococcus faecium isolated from urinary tract infection in Isfahan, Iran

Gene Reports ◽  
2018 ◽  
Vol 13 ◽  
pp. 72-75 ◽  
Author(s):  
Nematolah Rahimi ◽  
Farkhondeh Poursina ◽  
Faezeh sadat Ghaziasgar ◽  
Shima Sepehrpor ◽  
Akbar Hassanzadeh
Keyword(s):  
Urinary Tract Infection ◽  
Urinary Tract ◽  
Tract Infection ◽  
Enterococcus Faecalis ◽  
Virulence Factor ◽  
Enterococcus Faecium ◽  
Biofilm Formation ◽  
Virulence Factor Genes

scholarly journals Utility of Chromogenic Medium in Characterization of Enterococci in Urinary Tract Infection and Phenotypic Detection of Their Virulence Factors

2021 ◽  
Vol 11 (5) ◽  
pp. 278-283
Author(s):  
Betu Rama Soujanya ◽  
Banashankari G S
Keyword(s):  
Urinary Tract Infection ◽  
Urinary Tract ◽  
Tract Infection ◽  
Enterococcus Faecalis ◽  
Virulence Factors ◽  
Enterococcus Faecium ◽  
Antibiotic Sensitivity ◽  
Morbidity And Mortality ◽  
Enterococcus Species ◽  
Chromogenic Medium

Introduction: Enterococci from being intestinal commensals have evolved in becoming pathogens and are associated with significant morbidity and mortality Aims & Objectives: This study was done to speciate the uropathogenic Enterococci using the chromogenic medium and to determine the antibiogram also to detect virulence factors phenotypically. Materials and methods: The study included a total of 30 uropathogenic Enterococci isolated over 6 months. Speciation was done using HiCrome Enterococcus faecium agar base. Antibiotic sensitivity was done by the Kirby Bauer Disc Diffusion method as per Clinical and Laboratory Standards Institute (CLSI) guidelines. Among the virulence factors hemolysin, haemagglutination, and gelatin liquefaction tests were done. Results: Amongst the 30 enterococci isolates, 17 were Enterococcus faecalis (E. faecalis) (56.66%) & 13 were Enterococcus faecium (E. faecium) (43.33 %). 100% of the Enterococcus species were sensitive to Vancomycin & Teicoplanin. 66.67% of the Enterococci showed hemolysis, 10% haemagglutination, and 43.33% gelatinase property. Conclusion: Most common isolated species were Enterococcus faecalis. The changing patterns of antibiotic sensitivity to Enterococci in patients with urinary tract infection possess difficulty in selection of the antibiotics. Failure to synergistic therapy is seen in cases of resistance to High-level Gentamicin. Therefore, speciation and antibiotic sensitivity patterns will help in setting up an empirical therapy and thereby help in the reduction of morbidity and mortality. Key words: Antibiotic susceptibility, Chrome agar, Enterococcus species, virulence factors.

scholarly journals Contribution of Individual Ebp Pilus Subunits of Enterococcus faecalis OG1RF to Pilus Biogenesis, Biofilm Formation and Urinary Tract Infection

PLoS ONE ◽  
2013 ◽  
Vol 8 (7) ◽  
pp. e68813 ◽  
Author(s):  
Jouko Sillanpää ◽  
Chungyu Chang ◽  
Kavindra V. Singh ◽  
Maria Camila Montealegre ◽  
Sreedhar R. Nallapareddy ◽  
...  
Keyword(s):  
Urinary Tract Infection ◽  
Urinary Tract ◽  
Tract Infection ◽  
Enterococcus Faecalis ◽  
Biofilm Formation ◽  
Pilus Biogenesis

scholarly journals Involvement of Chromosomally Encoded Homologs of the RRNPP Protein Family in Enterococcus faecalis Biofilm Formation and Urinary Tract Infection Pathogenesis

2020 ◽  
Vol 202 (17) ◽  
Author(s):  
Srivatsan Parthasarathy ◽  
Lorne D. Jordan ◽  
Nancy Schwarting ◽  
Megan A. Woods ◽  
Zakria Abdullahi ◽  
...  
Keyword(s):  
Urinary Tract Infection ◽  
Urinary Tract ◽  
Tract Infection ◽  
Enterococcus Faecalis ◽  
Biofilm Formation ◽  
Family Members ◽  
Nutrient Sensing ◽  
Protein Family ◽  
Content Type ◽  
Tract Infections

ABSTRACT Enterococcus faecalis is an opportunistic pathogen capable of causing infections, including endocarditis and urinary tract infections (UTI). One of the well-characterized quorum-sensing pathways in E. faecalis involves coordination of the conjugal transfer of pheromone-responsive plasmids by PrgX, a member of the RRNPP protein family. Members of this protein family in various Firmicutes have also been shown to contribute to numerous cellular processes, including sporulation, competence, conjugation, nutrient sensing, biofilm formation, and virulence. As PrgX is a plasmid-encoded RRNPP family member, we surveyed the genome of the multidrug-resistant strain V583 for additional RRNPP homologs using computational searches and refined those identified hits for predicted structural similarities to known RRNPP family members. This led us to investigate the contribution of the chromosomally encoded RRNPP homologs to biofilm processes and pathogenesis in a catheter-associated urinary tract infection (CAUTI) model. In this study, we identified five such homologs and report that 3 of the 5 homologs, EF0073, EF1599, and EF1316, affect biofilm formation as well as outcomes in the CAUTI model. IMPORTANCE Enterococcus faecalis causes health care-associated infections and displays resistance to a variety of broad-spectrum antibiotics by acquisition of resistance traits as well as the ability to form biofilms. Even though a growing number of factors related to biofilm formation have been identified, mechanisms that contribute to biofilm formation are still largely unknown. Members of the RRNPP protein family regulate a diverse set of biological reactions in low-G+C Gram-positive bacteria (Firmicutes). Here, we identify three predicted structural homologs of the RRNPP family, EF0073, EF1599, and EF1316, which affect biofilm formation and CAUTI pathogenesis.

scholarly journals Relative Contributions of Enterococcus faecalis OG1RF Sortase-Encoding Genes, srtA and bps (srtC), to Biofilm Formation and a Murine Model of Urinary Tract Infection

2007 ◽  
Vol 75 (11) ◽  
pp. 5399-5404 ◽  
Author(s):  
Kelvin D. Kemp ◽  
Kavindra V. Singh ◽  
Sreedhar R. Nallapareddy ◽  
Barbara E. Murray
Keyword(s):  
Urinary Tract Infection ◽  
Urinary Tract ◽  
Tract Infection ◽  
Enterococcus Faecalis ◽  
Biofilm Formation ◽  
Double Mutant ◽  
Major Effect ◽  
Pilus Biogenesis ◽  
Surface Localization

ABSTRACT Deletion mutants of the two sortase genes of Enterococcus faecalis OG1RF were constructed. srtC (renamed here bps for biofilm and pilus-associated sortase) was previously shown to be necessary for the production of Ebp pili and important for biofilm formation and endocarditis. Here, we report that a srtA deletion mutant showed a small (5%) yet significant (P = 0.037) reduction in biofilm relative to OG1RF, while a ΔsrtA Δbps double mutant showed a much greater reduction (74% versus OG1RF and 44% versus the Δbps mutant). In a murine urinary tract infection (UTI), the 50% infective doses of both the ΔsrtA Δbps and Δbps mutants were ∼2 log10 greater than that of OG1RF or the ΔsrtA mutant. Similarly, ∼2 log10 fewer bacteria were recovered from the kidneys after infection with the Δbps mutant (P = 0.017) and the ΔsrtA Δbps double mutant (P = 0.022) compared to wild-type strain OG1RF. In a competition UTI, the Δbps mutant was slightly, but not significantly, less attenuated than the ΔsrtA Δbps double mutant. Fluorescence-activated cell sorter analysis with Ebp-specific antibodies confirmed that a minority of OG1RF cells express Ebp pili on their surface in vitro and that Bps has a major role in Ebp pilus biogenesis but also indicated a function for SrtA in surface localization of the pilus subunit protein EbpA. In conclusion, deletion of bps had a major effect on virulence in murine UTIs, as well as biofilm; deletion of srtA from OG1RF had little effect on these phenotypes, but its deletion from a bps mutant had a pronounced effect on biofilm, suggesting that Bps and/or the proteins it anchors may compensate for the loss of some SrtA function(s).

The effect of some chemical materials against virulence factors of Candida ssp isolated from UTI patients

2018 ◽  
Vol 9 (SPL1) ◽  
Author(s):  
Fatima Abdul Hussein Mejbel
Keyword(s):  
Urinary Tract Infection ◽  
Urinary Tract ◽  
Tract Infection ◽  
Virulence Factors ◽  
Biofilm Formation ◽  
Germ Tube ◽  
Laboratory Culture ◽  
Candida Spp ◽  
Other Substances ◽  
Chemical Materials

 During the period from September 2016 to December 2017,135 urine samples were collected from urinary tract infection patients attending to AL-Zahraa Hospital in AL-Najaf Governorate. The present study was conducted to isolate and identify Candida spp. isolated from urinary tract infection patients by different methods including direct examination, laboratory culture, biochemical test and by modern techniques (Api Candida kit) and determine the virulence factors phenotypic to Candida spp which involved (biofilm formation,phospholipase and germ tube). The percentage of females to males was as following, female (84) 62.2 % (21) infected and male (51) 37.8% (1) infected with all age categories. The results in this study are explain that is some Candida spp. such as C. albicans, have high susceptible to eugenole follow by phenol and umbellulone. The efficiency of some chemical substances such as (eugenole,umbellulone, and phenol) was evaluated to inhibit the growth of Candida ssp as well as some virulence factors such as biofilm formation,germ tube and phospholipase,which were studied in this research. Statistically analysis results have been significance difference between the results of the substance concentrations and the concentrations of the different other substances.

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