Transportation and augmentation of the deposited soil bacteria in the electrokinetic process: Interactions between soil particles and bacteria

Geoderma ◽  
2021 ◽  
Vol 404 ◽  
pp. 115260
Author(s):  
Fenglian Cheng ◽  
Shuhai Guo ◽  
Sa Wang ◽  
Penghong Guo ◽  
Wenjie Lu
Keyword(s):  
Soil Bacteria ◽  
Soil Particles ◽  
Electrokinetic Process

Enumeration of soil bacteria with the green fluorescent nucleic acid dye Sytox green in the presence of soil particles

2004 ◽  
Vol 59 (2) ◽  
pp. 189-198 ◽  
Author(s):  
Peter Klauth ◽  
Ralf Wilhelm ◽  
Erwin Klumpp ◽  
Lothar Poschen ◽  
Joost Groeneweg
Keyword(s):  
Nucleic Acid ◽  
Soil Bacteria ◽  
Acid Dye ◽  
Soil Particles ◽  
Sytox Green ◽  
Green Fluorescent

Some Bacteriological Relations in Soils kept under Green-house conditions

1910 ◽  
Vol 3 (3) ◽  
pp. 301-310 ◽  
Author(s):  
Jacob G. Lipman ◽  
Irving L. Owen
Keyword(s):  
Organic Matter ◽  
Soil Bacteria ◽  
Atmospheric Oxygen ◽  
Intimate Contact ◽  
Soil Particles ◽  
Green House ◽  
Direct Knowledge ◽  
Field Soils ◽  
Number Of Microorganisms ◽  
Number Of Bacteria

Soils in the green-house are exposed to conditions that are admittedly more or less artificial. The range of temperature and moisture is not the same as that in field soils, while the aeration of green-house soils is, if anything, even more artificial. The artificial conditions are emphasized still more strongly when the soil is kept in small pots. The operations incident to the filling of the pots and the applications of fertilizers involve a more intimate contact of the soil particles with atmospheric oxygen than is possible under field or garden conditions. This leads to an abnormal multiplication of the soil bacteria and to a consequent abnormally rapid oxidation of the organic matter. In the course of time the more readily decomposable portions of the organic matter in the soil become depleted and this is followed, in turn, by a decline in the numbers of bacteria that will grow on agar plates. It is possible that the rapid falling off in numbers is due not merely to the depletion of the readily decomposable organic matter, but also to the accumulation of certain cleavage products injurious to the bacteria. Of the latter factor we have no direct knowledge and it is referred to in this place only as a possible explanation of the facts recorded below. It may be added here, also, that the greatly decreased number of bacteria appearing on agar plates should not be accepted as absolute proof that the total number of microorganisms in the soil had diminished. There is a possibility that a compensating increase had occurred of bacteria that do not grow on agar plates, as for instance, the nitrous and nitric ferments, etc.….

scholarly journals Revisiting soil bacterial counting methods: Optimal soil storage and pretreatment methods and comparison of culture-dependent and -independent methods

PLoS ONE ◽  
2021 ◽  
Vol 16 (2) ◽  
pp. e0246142
Author(s):  
Jeonggil Lee ◽  
Han-Suk Kim ◽  
Ho Young Jo ◽  
Man Jae Kwon
Keyword(s):  
Soil Bacteria ◽  
Epifluorescence Microscopy ◽  
Most Probable Number ◽  
Soil Matrix ◽  
Probable Number ◽  
Soil Particles ◽  
Soil Storage ◽  
Dna Quantitation ◽  
Soil Bacterial ◽  
Culture Dependent

Although a number of different methods have been used to quantify soil bacteria, identifying the optimal method(s) for soil bacterial abundance is still in question. No single method exists for undertaking an absolute microbial count using culture-dependent methods (CDMs) or even culture-independent methods (CIMs). This study investigated soil storage and pretreatment methods for optimal bacterial counts. Appropriate storage temperature (4°C) and optimal pretreatment methods (sonication time for 3 min and centrifugation at 1400 g) were necessary to preserve bacterial cell viability and eliminate interference from soil particles. To better estimate soil bacterial numbers under various cellular state and respiration, this study also evaluated three CDMs (i.e., colony forming unit, spotting, and most probable number (MPN) and three CIMs (i.e., flow cytometry (FCM), epifluorescence microscopy (EM) count, and DNA quantitation). Each counting method was tested using 72 soil samples collected from a local arable farm site at three different depths (i.e., 10–20, 90–100, and 180–190 cm). Among all CDMs, MPN was found to be rapid, simple, and reliable. However, the number of bacteria quantified by MPN was 1–2 orders lower than that quantified by CIMs, likely due to the inability of MPN to count anaerobic bacteria. The DNA quantitation method appeared to overestimate soil bacterial numbers, which may be attributed to DNA from dead bacteria and free DNA in the soil matrix. FCM was found to be ineffective in counting soil bacteria as it was difficult to separate the bacterial cells from the soil particles. Dyes used in FCM stained the bacterial DNA and clay particles. The EM count was deemed a highly effective method as it provided information on soil mineral particles, live bacteria, and dead bacteria; however, it was a time-consuming and labor-intensive process. Combining both types of methods was considered the best approach to acquire better information on the characteristics of indigenous soil microorganisms (aerobic versus anaerobic, live versus dead).

DIVERSITY OF SOIL BACTERIA AS INDICATOR OF SOIL POLLUTION IN MOLDAVIA REGION, ROMANIA

2017 ◽  
Vol 16 (4) ◽  
pp. 879-889 ◽  
Author(s):  
Florin Daniel Lipsa ◽  
Eugen Ulea ◽  
Andreea Mihaela Balau ◽  
Feodor Filipov ◽  
Evelina Cristina Morari
Keyword(s):  
Soil Pollution ◽  
Soil Bacteria

Isolasi dan Karakterisasi Bakteri Tanah Sawah di Kecamatan Medan Satria dan Bekasi Utara, Kota Bekasi, Jawa Barat

2017 ◽  
Vol 3 (4) ◽  
pp. 187 ◽  
Author(s):  
Arief Pambudi ◽  
Nita Noriko ◽  
Endah Permata Sari
Keyword(s):  
Plant Growth ◽  
Soil Bacteria ◽  
Rice Field ◽  
Plant Growth Promoting Rhizobacteria ◽  
Fertilizer Application ◽  
Rice Production ◽  
Production Costs ◽  
Soil Conditions ◽  
Nitrogen Fixation Activity ◽  
Growth Promoting

<p><em>Abstrak -</em><strong> </strong><strong>Produksi padi di Indonesia setiap tahun mengalami peningkatan, namun peningkatan ini belum mampu memenuhi kebutuhan nasional sehingga impor masih harus dilakukan. Salah satu masalah dalam produksi beras adalah penggunaan pupuk berlebih yang tidak hanya meningkatkan biaya produksi, namun juga merusak kondisi tanah. Aplikasi bakteri tanah sebagai Plant <em>Growth Promoting Rhizobacteria</em> (PGPR) dapat menjadi salah satu solusi terhadap masalah ini. Penelitian ini bertujuan untuk mengisolasi bakteri tanah dari 3 lokasi sawah daerah Bekasi, membandingkan keberadaan total bakteri pada ketiga lokasi tersebut,  dan melakukan karakterisasi isolat berdasarkan karakter yang dapat memicu pertumbuhan tanaman. Dari ketiga lokasi, diperoleh total 59 isolat dan 5 diantaranya berpotensi sebagai PGPR karena kemampuan fiksasi Nitrogen, melarutkan Fosfat, katalase positif, dan motil. Dari ketiga lokasi pengambilan sampel, BK1 memiliki jumlah total bakteri terendah karena aplikasi pemupukan dan pestisida berlebih yang ditandai tingginya kadar P total, serta tingginya residu klorpirifos, karbofuran, dan paration. Kondisi fisik tanah BK1 juga didominasi partikel liat yang menyebabkan tanah menjadi lebih padat. Peningkatan jumlah penggunaan pupuk tidak selalu diikuti peningkatan produktivitas tanaman.</strong></p><p> </p><p><strong><em>Kata Kunci</em></strong><strong><em> </em></strong>- <em>Bakteri tanah, Rhizosfer sawah, PGPR, Pupuk Hayati</em></p><p><strong> </strong></p><p><em>Abstract</em><strong> - </strong><strong>Rice production in Indonesia has increased annually, but this increase has not reached national demand,so imports still done. </strong><strong>One of the problems in rice production is the use of excessive fertilizers that not only increase production costs, but also decreased the soil conditions. The application of soil bacteria as Plant Growth Promoting Rhizobacteria (PGPR) can be the one solution to face this problem. The objective of this study was isolate soil bacteria from 3 locations of rice field in Bekasi, compare the total bacteria in the three locations, and characterize isolates based on the character that can promote plant growth. From three locations, a total of 59 isolates were obtained and 5 of them were potential as a PGPRs due to its Nitrogen fixation activity, Phosphate solubilization, positive catalase, and motility. From three sampling sites, BK1 has the lowest TPC value because of excessive  fertilizers and pesticides application which indicated by high total P levels, and also high chlorpyrifos, carbofuran and paration residues. The physical condition of BK1 soil is also dominated by clay particles which causes the soil more solid. Increasing of fertilizer application is not always followed by increased plant productivity.</strong></p><p><strong> </strong></p><p><strong><em>Keywords</em></strong> - <em>Biofertilizer, PGPR, Rice field rhizosphere, Soil Bacteria</em></p>

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