Differential gene and protein expression in gastrocnemius and tibialis anterior muscle following tibial and peroneal nerve injury in rats

OBJECT The purpose of this study was to determine the minimum amount of nerve fibers required to maintain normal motor function after nerve injury in rats. METHODS The authors first confirmed that a common peroneal nerve injury caused more aggravating effects on lower limb motor function than tibial nerve injury, as assessed by the static sciatic index (SSI). Thereafter, rats were subjected to varying degrees of crush injury to the common peroneal nerve. At 48 hours after the injury, motor function was assessed using the SSI and slope-walking ability (with slope angles of 30° and 45°). The tibialis anterior muscle, a main muscle innervated by the common peroneal nerve, was removed. Muscle sections were co-labeled with neuronal class III β-tubulin polyclonal antibody to identify the presence of axons and Alexa Fluor 488-conjugated α-bungarotoxin to identify the presence of motor endplates. RESULTS The evaluation of neuromuscular innervation showed a correlation between SSI scores and ratios of residual axons (rs = 0.68, p < 0.01), and there was a statistically significant difference between slope-walking ability and ratios of residual axons (p < 0.01). Moreover, the ratios of residual axons in the nerve-crushed rats with normal motor function (SSI above −20) ranged from 36.5% to 88.7%, and those ratios in the success group with slope-walking angles of 30° and 45° ranged from 14.7% to 88.7% and from 39.8% to 88.7%, respectively. CONCLUSIONS In this study of rodents, less than half of the motor axons were sufficient to maintain normal motor function of the lower limb.

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Isokinetic cross-training effect in foot drop following common peroneal nerve injury

Isokinetics and Exercise Science ◽

10.3233/ies-140559 ◽

2015 ◽

Vol 23 (1) ◽

pp. 17-20 ◽

Cited By ~ 4

Author(s):

Andrea Manca ◽

Francesco Pisanu ◽

Enzo Ortu ◽

Edoardo De Natale ◽

Francesca Ginatempo ◽

...

Keyword(s):

Nerve Injury ◽

Peroneal Nerve ◽

Common Peroneal Nerve ◽

Foot Drop ◽

Training Effect ◽

Cross Training ◽

Peroneal Nerve Injury

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Iatrogenic common peroneal nerve injury during harvesting of semitendinosus tendon for anterior cruciate ligament reconstruction

BMJ Case Reports ◽

10.1136/bcr-2020-240736 ◽

2021 ◽

Vol 14 (4) ◽

pp. e240736

Author(s):

Raf Mens ◽

Albert van Houten ◽

Roy Bernardus Gerardus Brokelman ◽

Roy Hoogeslag

Keyword(s):

Anterior Cruciate Ligament ◽

Nerve Injury ◽

Peroneal Nerve ◽

Cruciate Ligament ◽

Common Peroneal Nerve ◽

Peroneal Nerve Injury ◽

The Common ◽

Anterior Cruciate ◽

Incision Technique

We present a case of iatrogenic injury to the common peroneal nerve (CPN) occurring due to harvesting of a hamstring graft, using a posterior mini-incision technique. A twitch of the foot was noted on retraction of the tendon stripper. After clinically diagnosing a CPN palsy proximal to the knee, the patient was referred to a neurosurgeon within 24 hours. An electromyography (EMG) was not obtained since it cannot accurately differentiate between partial and complete nerve injury in the first week after injury. Because the nerve might have been transacted by the tendon stripper, surgical exploration within 72 hours after injury was indicated. An intraneural haematoma was found and neurolysis was performed to decompress the nerve. Functioning of the anterior cruciate ligament was satisfactory during follow-up. Complete return of motor function of the CPN was observed at 1-year follow-up, with some remaining hypoaesthesia.

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Anatomic risk of peroneal nerve injury with the “pie crust” technique for valgus release in total knee arthroplasty

The Journal of Arthroplasty ◽

10.1016/j.arth.2003.09.004 ◽

2004 ◽

Vol 19 (1) ◽

pp. 40-44 ◽

Cited By ~ 42

Author(s):

Henry D Clarke ◽

Jonathan B Schwartz ◽

Kevin R Math ◽

Giles R Scuderi

Keyword(s):

Total Knee Arthroplasty ◽

Nerve Injury ◽

Knee Arthroplasty ◽

Peroneal Nerve ◽

Peroneal Nerve Injury ◽

Total Knee

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Poster 143: Peroneal Nerve Injury and Foot Drop Following Tibia and Fibula Fracture: A Case Report

Archives of Physical Medicine and Rehabilitation ◽

10.1016/j.apmr.2007.06.501 ◽

2007 ◽

Vol 88 (9) ◽

pp. E49-E50

Author(s):

Anupam Sinha ◽

Lyn Weiss

Keyword(s):

Case Report ◽

Nerve Injury ◽

Peroneal Nerve ◽

Foot Drop ◽

Fibula Fracture ◽

Peroneal Nerve Injury

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An experimental study to determine and correlate choline acetyltransferase assay with functional muscle testing after nerve injury

Journal of Neurosurgery ◽

10.3171/2014.1.jns122241 ◽

2014 ◽

Vol 120 (5) ◽

pp. 1125-1130 ◽

Cited By ~ 1

Author(s):

Torpon Vathana ◽

Tim H. J. Nijhuis ◽

Patricia F. Friedrich ◽

Allen T. Bishop ◽

Alexander Y. Shin

Keyword(s):

Tibialis Anterior ◽

Peroneal Nerve ◽

Force Measurement ◽

Tibialis Anterior Muscle ◽

Muscle Weight ◽

Tetanic Force ◽

Chat Activity ◽

Significant Difference ◽

Group 3 ◽

Group 2

Object Choline acetyltransferase (ChAT) is an enzyme synthesized within the body of a motor neuron whose role is to form the neurotransmitter acetylcholine. Quantification of ChAT levels in motor or mixed nerves has been proposed to provide information regarding the viability of a proximal nerve stump for motor neurotization following brachial plexus injury. To do so requires information regarding normal ChAT levels and those in injured nerves, as well as the correlation of ChAT level determined at surgery with eventual motor recovery. The purpose of this study was to determine ChAT activity in the normal and injured sciatic/peroneal nerve in a rat model, evaluate the correlation between ChAT and motor recovery, find the relationship between ChAT activity and isometric muscle force, and elucidate the parallel between ChAT activity and acetylcholinesterase (AChE) activity. Methods Sixty animals were divided into 3 groups. The sciatic nerves in Group 1 were transected without repair. Nerves in Group 2 were transected and repaired. Nerves in Group 3 sustained a crush injury followed by transection and reconstruction. All animals were allowed 12 weeks of recovery followed by evaluation of ChAT levels in the peroneal nerve, correlated with measures of maximal isometric tibialis anterior muscle force and muscle weight (the operated side normalized to the control side). Karnovsky AChE staining of peroneal nerve segments was also compared with radiochemical assay of ChAT activity in the same nerve. Results A significant difference in the tibialis anterior isometric tetanic force and the tibialis anterior muscle weight index (TAMI) was noted between Group 1 and Groups 2 and 3 (p < 0.0001); no significant difference was found comparing Group 2 with Group 3. The correlation between the force measurement and the TAMI was 0.382. Both AChE measurement and ChAT activity demonstrated significantly fewer fibers in the operated nerve compared with the contralateral nerve. Intergroup variability could also be illustrated using these tests. The correlation coefficient between the isometric tetanic force measurement and the ChAT analysis in Groups 1 and 2 was 0.468. The correlation for the AChE staining and the isometric tetanic force measurement was 0.111. The correlation between the TAMI and the ChAT levels was 0.773. The correlation between the TAMI and the AChE-stained fibers was 0.640. Correlating AChE staining to the ChAT analysis produced a correlation of 0.712. Conclusions The great variability in all groups and weak correlations to the functional muscle assessments and the ChAT radiochemical assay made this technique an unreliable method of determining motor nerve viability.

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