Tu1939 FLUORESCENCE IMAGING OF EARLY ADENOCARCINOMA OF THE ESOPHAGOGASTRIC JUNCTION BY TOPICALLY SPRAYING PROBE TARGETING DIPEPTIDYLPEPTIDASE-IV

2019 ◽  
Vol 89 (6) ◽  
pp. AB624
Author(s):  
Keiko Yamamoto ◽  
Shunsuke Ohnishi ◽  
Takeshi Mizushima ◽  
Junichi Kodaira ◽  
Masayoshi Ono ◽  
...  
Keyword(s):  
Fluorescence Imaging ◽  
Esophagogastric Junction ◽  
Dipeptidylpeptidase Iv ◽  
Early Adenocarcinoma

scholarly journals Detection of Early Adenocarcinoma of the Esophagogastric Junction by Spraying an Enzyme-Activatable Fluorescent Probe Targeting Dipeptidyl Peptidase-IV

2019 ◽  
Author(s):  
Keiko Yamamoto ◽  
Shunsuke Ohnishi ◽  
Takeshi Mizushima ◽  
Junichi Kodaira ◽  
Masayoshi Ono ◽  
...  
Keyword(s):  
Intestinal Metaplasia ◽  
Fluorescent Probe ◽  
Fluorescence Imaging ◽  
Esophagogastric Junction ◽  
Dipeptidyl Peptidase ◽  
Clinical Samples ◽  
Specific Sequence ◽  
Dpp Iv ◽  
Early Adenocarcinoma ◽  
Sensitivity Specificity

Abstract Background: It is still difficult to detect and diagnose early adenocarcinoma of the esophagogastric junction (EGJ) using conventional endoscopy or image-enhanced endoscopy. A glutamylprolyl hydroxymethyl rhodamine green (EP-HMRG) fluorescent probe that can be enzymatically activated to become fluorescent after the cleavage of a dipeptidyl peptidase (DPP)-IV-specific sequence has been developed and is reported to be useful for the detection of squamous cell carcinoma of the head and neck, and esophagus; however, there is a lack of studies that focuses on detecting EGJ adenocarcinoma by fluorescence molecular imaging. Therefore, we investigated the visualization of early EGJ adenocarcinoma by applying EP-HMRG and using clinical samples resected by endoscopic submucosal dissection (ESD). Methods: Fluorescence imaging with EP-HMRG was performed in 21 clinical samples resected by ESD, and the fluorescence intensity of the tumor and non-tumor regions of interest was prospectively measured. Immunohistochemistry was also performed to determine the expression of DPP-IV. Results: Fluorescence imaging of the clinical samples showed that the tumor lesions were visualized within a few minutes after the application of EP-HMRG, with a sensitivity, specificity, and accuracy of 85.7%, 85.7%, and 85.7%, respectively. However, tumors with a background of intestinal metaplasia did not have a sufficient contrast-to-background ratio since complete intestinal metaplasia also expresses DPP-IV. Immunohistochemistry measurements revealed that all fluorescent tumor lesions expressed DPP-IV. Conclusions: Fluorescence imaging with EP-HMRG could be useful for the detection of early EGJ adenocarcinoma lesions that do not have a background of intestinal metaplasia.

scholarly journals Detection of Early Adenocarcinoma of the Esophagogastric Junction by Spraying an Enzyme-Activatable Fluorescent Probe Targeting Dipeptidyl Peptidase-IV

2020 ◽  
Author(s):  
Keiko Yamamoto ◽  
Shunsuke Ohnishi ◽  
Takeshi Mizushima ◽  
Junichi Kodaira ◽  
Masayoshi Ono ◽  
...  
Keyword(s):  
Intestinal Metaplasia ◽  
Fluorescent Probe ◽  
Fluorescence Imaging ◽  
Esophagogastric Junction ◽  
Dipeptidyl Peptidase ◽  
Clinical Samples ◽  
Specific Sequence ◽  
Dpp Iv ◽  
Early Adenocarcinoma ◽  
Sensitivity Specificity

Abstract Background: It is still difficult to detect and diagnose early adenocarcinoma of the esophagogastric junction (EGJ) using conventional endoscopy or image-enhanced endoscopy. A glutamylprolyl hydroxymethyl rhodamine green (EP-HMRG) fluorescent probe that can be enzymatically activated to become fluorescent after the cleavage of a dipeptidyl peptidase (DPP)-IV-specific sequence has been developed and is reported to be useful for the detection of squamous cell carcinoma of the head and neck, and esophagus; however, there is a lack of studies that focuses on detecting EGJ adenocarcinoma by fluorescence molecular imaging. Therefore, we investigated the visualization of early EGJ adenocarcinoma by applying EP-HMRG and using clinical samples resected by endoscopic submucosal dissection (ESD). Methods: Fluorescence imaging with EP-HMRG was performed in 21 clinical samples resected by ESD, and the fluorescence intensity of the tumor and non-tumor regions of interest was prospectively measured. Immunohistochemistry was also performed to determine the expression of DPP-IV. Results: Fluorescence imaging of the clinical samples showed that the tumor lesions were visualized within a few minutes after the application of EP-HMRG, with a sensitivity, specificity, and accuracy of 85.7%, 85.7%, and 85.7%, respectively. However, tumors with a background of intestinal metaplasia did not have a sufficient contrast-to-background ratio since complete intestinal metaplasia also expresses DPP-IV. Immunohistochemistry measurements revealed that all fluorescent tumor lesions expressed DPP-IV. Conclusions: Fluorescence imaging with EP-HMRG could be useful for the detection of early EGJ adenocarcinoma lesions that do not have a background of intestinal metaplasia.

scholarly journals Detection of Early Adenocarcinoma of the Esophagogastric Junction by Spraying an Enzyme-Activatable Fluorescent Probe Targeting Dipeptidyl Peptidase-IV

2019 ◽  
Author(s):  
Keiko Yamamoto ◽  
Shunsuke Ohnishi ◽  
Takeshi Mizushima ◽  
Junichi Kodaira ◽  
Masayoshi Ono ◽  
...  
Keyword(s):  
Intestinal Metaplasia ◽  
Fluorescent Probe ◽  
Fluorescence Imaging ◽  
Esophagogastric Junction ◽  
Dipeptidyl Peptidase ◽  
Clinical Samples ◽  
Specific Sequence ◽  
Dpp Iv ◽  
Early Adenocarcinoma ◽  
Sensitivity Specificity

Abstract Background: It is still difficult to detect and diagnose early adenocarcinoma of the esophagogastric junction (EGJ) using conventional endoscopy or image-enhanced endoscopy. A glutamylprolyl hydroxymethyl rhodamine green (EP-HMRG) fluorescent probe that can be enzymatically activated to become fluorescent after the cleavage of a dipeptidyl peptidase (DPP)-IV-specific sequence has been developed and is reported to be useful for the detection of squamous cell carcinoma of the head and neck, and esophagus; however, there is a lack of studies that focuses on detecting EGJ adenocarcinoma by fluorescence molecular imaging. Therefore, we investigated the visualization of early EGJ adenocarcinoma by applying EP-HMRG and using clinical samples resected by endoscopic submucosal dissection (ESD). Methods: Fluorescence imaging with EP-HMRG was performed in 21 clinical samples resected by ESD, and the fluorescence intensity of the tumor and non-tumor regions of interest was prospectively measured. Immunohistochemistry was also performed to determine the expression of DPP-IV. Results: Fluorescence imaging of the clinical samples showed that the tumor lesions were visualized within a few minutes after the application of EP-HMRG, with a sensitivity, specificity, and accuracy of 85.7%, 85.7%, and 85.7%, respectively. However, tumors with a background of intestinal metaplasia did not have a sufficient contrast-to-background ratio since complete intestinal metaplasia also expresses DPP-IV. Immunohistochemistry measurements revealed that all fluorescent tumor lesions expressed DPP-IV. Conclusions: Fluorescence imaging with EP-HMRG could be useful for the detection of early EGJ adenocarcinoma lesions that do not have a background of intestinal metaplasia.

A Case of Early Adenocarcinoma at Esophagogastric Junction Treated with Cap-pitted Endoscopic Mucosal Resection

2012 ◽  
Vol 35 (2) ◽  
pp. 124
Author(s):  
Ye Ri So ◽  
Dae Ho Kim ◽  
Hyun A Yun ◽  
Hwa Young Seok ◽  
Seong Hun Hong ◽  
...  
Keyword(s):  
Endoscopic Mucosal Resection ◽  
Esophagogastric Junction ◽  
Mucosal Resection ◽  
Early Adenocarcinoma

390 STRATEGY FOR TREATMENT OF EARLY ADENOCARCINOMA (T1) OF THE ESOPHAGOGASTRIC JUNCTION ACCORDING TO LYMPH NODE METASTASIS STATUS AND VASCULAR INVASION

2021 ◽  
Vol 34 (Supplement_1) ◽  
Author(s):  
Kosuke Narumiya ◽  
Kenji Kudo ◽  
Shinsuke Maeda ◽  
Yukinori Toyoshima ◽  
Kyohei Ogawa ◽  
...  
Keyword(s):  
Lymph Node ◽  
Lymph Node Metastasis ◽  
Lymph Node Dissection ◽  
Vascular Invasion ◽  
Esophagogastric Junction ◽  
Lymphatic Invasion ◽  
Depth Of Invasion ◽  
Node Dissection ◽  
Node Metastasis ◽  
Early Adenocarcinoma

Abstract   Incidence of adenocarcinoma of the esophagogastric junction is increasing in Japan. However, in early cases (T1), there is no consensus on treatment strategy. The purpose of this study was to determine the optimal range of resection and lymph node dissection according to lymph node metastasis status and vascular invasion in early adenocarcinoma (T1) of the esophagogastric junction. Methods We investigated patient characteristics, surgical procedures, recurrence pattern, and optimum extent of lymph node dissection in 22 patients who underwent surgery in our hospital from 2000 to 2016 and were diagnosed with early adenocarcinoma of the esophagogastric junction (by Nishi’s classification). Results Four patients with lymph node metastasis, the depth of invasion was sm2 and lymphatic invasion was positive (ly1–ly3, focal lymphatic invasion to prominent lymphatic invasion). In all cases, the site of lymph node metastasis was the lesser gastric curvature. None of the patients developed postoperative lymph node recurrence. An examination of the outcomes revealed that the metastases were hematogenous in all patients with a depth of invasion of sm2 and a positive venous invasion (v1, focal vascular invasion). Conclusion We conclude that transhiatal esophagectomy should be selected as a minimal requirement, and that dissection of the abdominal lymph node (particularly on the lesser curvature side of the superior part of the stomach) is sufficient, for patients with early adenocarcinoma of the esophagogastric junction. In cases where the depth of invasion is sm2 or greater and vascular invasion is present, patients may require adjuvant therapy regardless of lymph node metastasis status.

scholarly journals Thoracoscopic esophagectomy is related to better outcomes in early adenocarcinoma of esophagogastric junction tumors

2021 ◽  
Vol 13 (8) ◽  
pp. 319-328
Author(s):  
Flavio Roberto Takeda ◽  
Carlos de Almeida Obregon ◽  
Yasmin Peres Navarro ◽  
Diogo Turiani Hourneaux Moura ◽  
Ulysses Ribeiro Jr ◽  
...  
Keyword(s):  
Esophagogastric Junction ◽  
Thoracoscopic Esophagectomy ◽  
Adenocarcinoma Of Esophagogastric Junction ◽  
Early Adenocarcinoma

The influence of confocal microscope design on imaging performance

1993 ◽  
Vol 51 ◽  
pp. 144-145
Author(s):  
C J R Sheppard
Keyword(s):  
Image Quality ◽  
Fluorescence Imaging ◽  
Confocal Microscope ◽  
Industrial Applications ◽  
Three Dimensions ◽  
Design Parameters ◽  
Weak Signals ◽  
Size And Shape ◽  
Imaging Performance ◽  
Fundamental Limitation

The confocal microscope is now widely used in both biomedical and industrial applications for imaging, in three dimensions, objects with appreciable depth. There are now a range of different microscopes on the market, which have adopted a variety of different designs. The aim of this paper is to explore the effects on imaging performance of design parameters including the method of scanning, the type of detector, and the size and shape of the confocal aperture.It is becoming apparent that there is no such thing as an ideal confocal microscope: all systems have limitations and the best compromise depends on what the microscope is used for and how it is used. The most important compromise at present is between image quality and speed of scanning, which is particularly apparent when imaging with very weak signals. If great speed is not of importance, then the fundamental limitation for fluorescence imaging is the detection of sufficient numbers of photons before the fluorochrome bleaches.

Ubiquinone-BODIPY nanoparticles for tumor redox-responsive fluorescence imaging and photodynamic activity

2021 ◽  
Author(s):  
Byunghee Hwang ◽  
Tae-Il Kim ◽  
Hyunjin Kim ◽  
Sungjin Jeon ◽  
Yongdoo Choi ◽  
...  
Keyword(s):  
Fluorescence Imaging ◽  
Selective Activation ◽  
Turn On ◽  
Intracellular Environment ◽  
Redox Responsive ◽  
Photodynamic Activity

A ubiquinone-BODIPY photosensitizer self-assembles into nanoparticles (PS-Q-NPs) and undergoes selective activation within the highly reductive intracellular environment of tumors, resulting in “turn-on” fluorescence and photosensitizing activities.

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