scholarly journals Organic solvent extraction and spectrophotometric quantification of total phenolic content of soil.

Heliyon ◽  
2021 ◽  
pp. e08388
Author(s):  
Nicholas Akomeng ◽  
Stephen Adusei
2016 ◽  
Vol 8 (3) ◽  
pp. 337
Author(s):  
Soumya Tumbath ◽  
Lakshmipriya Theyyankandi ◽  
Jayasree Pullampara Rajamma ◽  
Manish Kumar Panickassery Ramakrishnan

<p class="Default">Zingiberaceae family members are well known for their ethnobotanical diversity and medicinal importance.  This study aimed to evaluate total phenolic content, antioxidant and antiproliferative capacity of five different organic solvent extracts prepared from the rhizomes of <em>Curcuma mutabilis</em> (CM)<em>, Curcuma haritha </em>(CH)<em>, Curcuma neilgherrensis</em> (CN) and<em> Zingiber anamalayanum</em> (ZA), four hitherto unexplored Zingiberaceae species.  Folin-<span class="tgc">Ciocalteu method and </span>DPPH radical scavenging assay were used to determine respectively the total phenolic content and<span class="tgc"> antioxidant </span>capacity.<span class="tgc">  </span>The antiproliferative activity of the extracts were tested against four human cancer cell lines –  K562,  REH, Nalm6 and MCF7 to ascertain the IC<sub>50</sub> values.  Based on total phenolic content, extracts were classified into high-H (&gt; 150 mg GAE/g), medium-M (50-150 mg GAE/g) and low-L (&lt; 50 mg GAE/g) categories.  Likewise, percentages of DPPH scavenging activity of extracts were also grouped into high-H (&gt; 50%), medium-M (25 – 50%) and low-L (&lt; 25%) categories.  Ten of the twenty extracts exhibited strong cytotoxicity with an IC<sub>50 </sub>value less than 30 μg/mL.  To our knowledge, this is the first report on quantitative assessment of total phenolics, antioxidant and antiproliferative potential of organic solvent extracts of rhizomes from the above mentioned plants.</p>


2020 ◽  
Vol 11 (SPL4) ◽  
pp. 3042-3048
Author(s):  
Khusnul Ma’rifah ◽  
I Made Artika ◽  
Janson Calvindi ◽  
Waras Nurcholis

Cardamom (Amomum compactum  Sol. Ex Maton) is a medicinal plant that belongs to the family Zingiberaceae. Phenolic compound detected in Cardamom fruit that responsible for several pharmacological activities. Optimization used the simplex centroid design for the yield and phenolic extractions of Cardamom fruit through maceration to optimize the mixing of water, acetone, methanol, and ethanol. The extraction yield is determined by weighing the extracted material compared to the whole sample. Total phenolic content was measured by a spectrophotometric method using Folin-Ciocalteu reagent.  The results show that the best for relating extract yield is the linear model, while the quadratic model is the best to connect total phenolic content response. Water (100%) solvent extraction on Cardamom fruits obtained the highest extract yield (10.52%) and the lowest extract yield from 100% ethanol. The extraction of phenolic compounds with a mixture of water (50%)/ethanol (50%) resulted in maximum total phenolic content (168.98 mg GAE/g). However, the 100% ethanol of solvent extraction noted the minimum phenolic content (93.15 mg GAE/g). The results show that we should carefully choose the solvent mixture extraction to achieve the extract yield and phenolic extraction goals. This study first reported an optimization study on phenolic compounds extracted from Cardamom fruit.


Planta Medica ◽  
2016 ◽  
Vol 81 (S 01) ◽  
pp. S1-S381
Author(s):  
MA Ghareeb ◽  
T Mohamed ◽  
AM Saad ◽  
LA Refahy ◽  
MA Sobeh ◽  
...  

1972 ◽  
Vol 69 (2) ◽  
pp. 257-266 ◽  
Author(s):  
Bryant Benson ◽  
Mary Jane Matthews ◽  
Alvin E. Rodin

ABSTRACT Continuing investigation of pineal gland function indicates that the anti-gonadotrophic activity of this organ cannot be attributed solely to the postulated hormone melatonin, the concentration of which is negligible in the pineal body compared to quantities required to produce unequivocal physiological effects. A non-melatonin antigonadotrophic substance recently isolated from bovine pineal glands was further purified by organic solvent extraction, ultrafiltration and gel filtration. Studies of partial blockage of compensatory ovarian hypertrophy in unilaterally ovariectomized Charles River CD-1 mice indicated that this substance is significantly more potent than melatonin in this test system.


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