scholarly journals Clinical Evaluation of SARS-CoV-2 Antigen-based Rapid Diagnostic Test Kit for Detection of COVID-19 cases in Bangladesh

Heliyon ◽  
2021 ◽  
pp. e08455
Author(s):  
Md. Mahfuzur Rahman ◽  
Ananya Ferdous Hoque ◽  
Yeasir Karim ◽  
Zannat Kawser ◽  
Abu Bakar Siddik ◽  
...  
Keyword(s):  
Diagnostic Test ◽  
Rapid Diagnostic Test ◽  
Clinical Evaluation ◽  
Test Kit

scholarly journals Clinical Evaluation of Rapid Diagnostic Test Kit for Scrub Typhus with Improved Performance

2016 ◽  
Vol 31 (8) ◽  
pp. 1190 ◽  
Author(s):  
Young-Jin Kim ◽  
Sungman Park ◽  
Ranjan Premaratna ◽  
Stephen Selvaraj ◽  
Sang-Jin Park ◽  
...  
Keyword(s):  
Diagnostic Test ◽  
Rapid Diagnostic Test ◽  
Clinical Evaluation ◽  
Scrub Typhus ◽  
Test Kit ◽  
Improved Performance

scholarly journals Efficacy of a new rapid diagnostic test kit to diagnose Sri Lankan cutaneous leishmaniasis caused by Leishmania donovani

PLoS ONE ◽  
2017 ◽  
Vol 12 (11) ◽  
pp. e0187024 ◽  
Author(s):  
Gayani De Silva ◽  
Vijani Somaratne ◽  
Sujai Senaratne ◽  
Manuja Vipuladasa ◽  
Rajitha Wickremasinghe ◽  
...  
Keyword(s):  
Cutaneous Leishmaniasis ◽  
Diagnostic Test ◽  
Rapid Diagnostic Test ◽  
Leishmania Donovani ◽  
Sri Lankan ◽  
Test Kit

scholarly journals Pengembangan Rapid Diagnostic Test Septicemia of Catfish (ESC) Pada Ikan Patin (Pangasius pangasius) Dengan Slide Aglutinasi Test

2020 ◽  
Vol 8 (1) ◽  
Author(s):  
Nanang Muhson ◽  
Iesje Lukistyowati ◽  
Windarti .
Keyword(s):  
Diagnostic Test ◽  
Rapid Diagnostic Test ◽  
Test Kit ◽  
Enteric Septicemia ◽  
Antigen Antibody

Aglutinasi merupakan reaksi antibodi dan antigen dapat digunakan sebagai dasar untuk mengembangkan test kit untuk mendeteksi keberadaan bakteri yang menyebabkan penyakit ikan dengan cepat. Untuk memahami apakah reaksi antigen-antibodi E. ictaluri dapat digunakan untuk mendeteksi penyakit Enteric Septicemia of Catfish (ESC), sebuah penelitian telah dilakukan dari September hingga Desember 2018. Antibodi diproduksi dari kelinci yang diimunisasi E. ictaluri yang tidak aktif (dosis bertingkat, periode 4 minggu, 0,5; 1; 2; dan 3 ml / minggu masing-masing). Serum Antibodi dipanen pada minggu ke-5. Antibodi itu kemudian digunakan untuk mendeteksi keberadaan E. ictaluri pada ikan yang terinfeksi. Sepuluh ikan patin diinfeksi dengan E. ictaluri aktif (1 ml, 106CFU / ikan, injeksi intraperitoneal). Tiga hari setelah infeksi dan ikan menunjukkan gejala penyakit ESC, isolat E. ictaluri diambil dengan mengekstraksi hati dan ginjal dan kemudian isolat tersebut ditanam di media TSA selama 24 jam. Isolat E. ictaluri kemudian disebarkan dalam kaca slide, dan dicampur dengan antibodi E. ictaluri. Reaksi antigen-antibodi itu dipelajari. Reaksi positif ditunjukkan oleh adanya butiran, sedangkan reaksi negatif tidak menghasilkan butiran-butiran seperti pasir. Hasil penelitian menunjukkan bahwa antigen E. ictaluri yang dikumpulkan dari ikan yang terinfeksi ESC bereaksi positif terhadap antigen E. ictaluri dan butiran terbentuk dalam waktu 1 menit setelah antigen - antibodi direaksikan. Antibodi E. ictaluri menunjukkan reaksi negatif terhadap antigen bakteri lain. The accuracy of Rapid Diagnostic Test based antigen – antibody agglutination is identic with the result of E. ictaluri detection using PCR. Berdasarkan data yang diperoleh, dapat disimpulkan bahwa Uji Aglutinasi Slide dapat digunakan sebagai Tes Diagnostik Cepat untuk mendeteksi penyakit ESC pada ikan patin.

scholarly journals Diagnostic performance of HRP2-only malaria rapid diagnostic test in Ghanaian pregnant women with asymptomatic peripheral blood infection: the case of First Response® test kit

2020 ◽  
Vol 1 (2) ◽  
pp. 86-92
Author(s):  
Joseph Osarfo ◽  
Harry Tagbor ◽  
Michael Alifragis ◽  
Pascal Magnuess
Keyword(s):  
Pregnant Women ◽  
Diagnostic Test ◽  
Rapid Diagnostic Test ◽  
Peripheral Blood ◽  
Diagnostic Performance ◽  
False Negative ◽  
Malaria Rapid Diagnostic Test ◽  
First Response ◽  
Test Kit ◽  
In Pregnancy

Background: The use of malaria rapid diagnostic test(RDT) enables targeted treatment that mitigates against the development of parasite drug resistance. With detection thresholds at ≥200 parasites/μL, their diagnostic performance in pregnant women may be challenging as asymptomatic infections with low parasite densities are common. Few data exist on the performance of histidine-rich protein-2 (HRP2) RDTs in Ghanaian pregnant women considering commonly occurring low parasite densities. Objective: The study sought to contribute more knowledge on test performance on First Response® test kit to guide the continuous evaluation of HRP2 RDTs in pregnancy. Methods: As part of an antimalarial drug trial in pregnancy, First Response® RDT results were compared to microscopy of peripheral blood slides in 1664 women. The diagnostic performance indicators were computed as proportions with 95% confidence intervals. The risk of having a positive RDT result was computed for age, gravidity and parasite density using binomial regression methods. Results: Parasitaemia prevalence by microscopy was 5.71%(n =95/1664) while that by RDT was 21.57%(n = 359/1664). Sensitivity was 82.11% (n=78/95), specificity was 82.09% (n=1288/1569), positive predictive value was 21.73% (n=78/359), and the likelihood ratio for a positive test was 4.58. False-negative RDT results were recorded for low parasite densities as well as densities ≥ 200/ μL while false-positive results were recorded in 281of 1664 tests. Primigravidae and younger aged women were more likely to have positive RDT results compared to multigravidae and women aged ≥ 30 yr. Conclusion: The moderate sensitivity, specificity and other diagnostic parameters reported suggest the First Response® malaria RDT is useful for detecting peripheral parasitaemia in pregnant women but the use of HRP2-only RDTs is limited by the existence of parasites with HRP2 gene deletion. The use of RDTs based on combined antigens continues to be recommended. Further research is needed on RDT performance in pregnant women with declining malaria transmission.

scholarly journals Comparison of Malaria Rapid Diagnostic Test Kit and Microscopy

2019 ◽  
Vol 10 (03) ◽  
pp. 109-115
Author(s):  
Oluwakemi Bukola Runmonkun ◽  
Omotayo Oluranti Ebong ◽  
Udeme Owunari Georgewill
Keyword(s):  
Diagnostic Test ◽  
Rapid Diagnostic Test ◽  
Malaria Rapid Diagnostic Test ◽  
Test Kit
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