scholarly journals Evaluation of Commercial Rapid Diagnostic Test Kit for Tuberculosis: Further Evidence Supporting Negative Policy on the Use of Serological Tests for Pulmonary Tuberculosis Diagnosis in Developing Countries

2013 ◽  
Vol 4 (2) ◽  
Keyword(s):  
Developing Countries ◽  
Pulmonary Tuberculosis ◽  
Diagnostic Test ◽  
Rapid Diagnostic Test ◽  
Serological Tests ◽  
Test Kit ◽  
Tuberculosis Diagnosis

scholarly journals Efficacy of a new rapid diagnostic test kit to diagnose Sri Lankan cutaneous leishmaniasis caused by Leishmania donovani

PLoS ONE ◽  
2017 ◽  
Vol 12 (11) ◽  
pp. e0187024 ◽  
Author(s):  
Gayani De Silva ◽  
Vijani Somaratne ◽  
Sujai Senaratne ◽  
Manuja Vipuladasa ◽  
Rajitha Wickremasinghe ◽  
...  
Keyword(s):  
Cutaneous Leishmaniasis ◽  
Diagnostic Test ◽  
Rapid Diagnostic Test ◽  
Leishmania Donovani ◽  
Sri Lankan ◽  
Test Kit

scholarly journals Pengembangan Rapid Diagnostic Test Septicemia of Catfish (ESC) Pada Ikan Patin (Pangasius pangasius) Dengan Slide Aglutinasi Test

2020 ◽  
Vol 8 (1) ◽  
Author(s):  
Nanang Muhson ◽  
Iesje Lukistyowati ◽  
Windarti .
Keyword(s):  
Diagnostic Test ◽  
Rapid Diagnostic Test ◽  
Test Kit ◽  
Enteric Septicemia ◽  
Antigen Antibody

Aglutinasi merupakan reaksi antibodi dan antigen dapat digunakan sebagai dasar untuk mengembangkan test kit untuk mendeteksi keberadaan bakteri yang menyebabkan penyakit ikan dengan cepat. Untuk memahami apakah reaksi antigen-antibodi E. ictaluri dapat digunakan untuk mendeteksi penyakit Enteric Septicemia of Catfish (ESC), sebuah penelitian telah dilakukan dari September hingga Desember 2018. Antibodi diproduksi dari kelinci yang diimunisasi E. ictaluri yang tidak aktif (dosis bertingkat, periode 4 minggu, 0,5; 1; 2; dan 3 ml / minggu masing-masing). Serum Antibodi dipanen pada minggu ke-5. Antibodi itu kemudian digunakan untuk mendeteksi keberadaan E. ictaluri pada ikan yang terinfeksi. Sepuluh ikan patin diinfeksi dengan E. ictaluri aktif (1 ml, 106CFU / ikan, injeksi intraperitoneal). Tiga hari setelah infeksi dan ikan menunjukkan gejala penyakit ESC, isolat E. ictaluri diambil dengan mengekstraksi hati dan ginjal dan kemudian isolat tersebut ditanam di media TSA selama 24 jam. Isolat E. ictaluri kemudian disebarkan dalam kaca slide, dan dicampur dengan antibodi E. ictaluri. Reaksi antigen-antibodi itu dipelajari. Reaksi positif ditunjukkan oleh adanya butiran, sedangkan reaksi negatif tidak menghasilkan butiran-butiran seperti pasir. Hasil penelitian menunjukkan bahwa antigen E. ictaluri yang dikumpulkan dari ikan yang terinfeksi ESC bereaksi positif terhadap antigen E. ictaluri dan butiran terbentuk dalam waktu 1 menit setelah antigen - antibodi direaksikan. Antibodi E. ictaluri menunjukkan reaksi negatif terhadap antigen bakteri lain. The accuracy of Rapid Diagnostic Test based antigen – antibody agglutination is identic with the result of E. ictaluri detection using PCR. Berdasarkan data yang diperoleh, dapat disimpulkan bahwa Uji Aglutinasi Slide dapat digunakan sebagai Tes Diagnostik Cepat untuk mendeteksi penyakit ESC pada ikan patin.

scholarly journals Diagnostic performance of HRP2-only malaria rapid diagnostic test in Ghanaian pregnant women with asymptomatic peripheral blood infection: the case of First Response® test kit

2020 ◽  
Vol 1 (2) ◽  
pp. 86-92
Author(s):  
Joseph Osarfo ◽  
Harry Tagbor ◽  
Michael Alifragis ◽  
Pascal Magnuess
Keyword(s):  
Pregnant Women ◽  
Diagnostic Test ◽  
Rapid Diagnostic Test ◽  
Peripheral Blood ◽  
Diagnostic Performance ◽  
False Negative ◽  
Malaria Rapid Diagnostic Test ◽  
First Response ◽  
Test Kit ◽  
In Pregnancy

Background: The use of malaria rapid diagnostic test(RDT) enables targeted treatment that mitigates against the development of parasite drug resistance. With detection thresholds at ≥200 parasites/μL, their diagnostic performance in pregnant women may be challenging as asymptomatic infections with low parasite densities are common. Few data exist on the performance of histidine-rich protein-2 (HRP2) RDTs in Ghanaian pregnant women considering commonly occurring low parasite densities. Objective: The study sought to contribute more knowledge on test performance on First Response® test kit to guide the continuous evaluation of HRP2 RDTs in pregnancy. Methods: As part of an antimalarial drug trial in pregnancy, First Response® RDT results were compared to microscopy of peripheral blood slides in 1664 women. The diagnostic performance indicators were computed as proportions with 95% confidence intervals. The risk of having a positive RDT result was computed for age, gravidity and parasite density using binomial regression methods. Results: Parasitaemia prevalence by microscopy was 5.71%(n =95/1664) while that by RDT was 21.57%(n = 359/1664). Sensitivity was 82.11% (n=78/95), specificity was 82.09% (n=1288/1569), positive predictive value was 21.73% (n=78/359), and the likelihood ratio for a positive test was 4.58. False-negative RDT results were recorded for low parasite densities as well as densities ≥ 200/ μL while false-positive results were recorded in 281of 1664 tests. Primigravidae and younger aged women were more likely to have positive RDT results compared to multigravidae and women aged ≥ 30 yr. Conclusion: The moderate sensitivity, specificity and other diagnostic parameters reported suggest the First Response® malaria RDT is useful for detecting peripheral parasitaemia in pregnant women but the use of HRP2-only RDTs is limited by the existence of parasites with HRP2 gene deletion. The use of RDTs based on combined antigens continues to be recommended. Further research is needed on RDT performance in pregnant women with declining malaria transmission.

scholarly journals Comparison of Malaria Rapid Diagnostic Test Kit and Microscopy

2019 ◽  
Vol 10 (03) ◽  
pp. 109-115
Author(s):  
Oluwakemi Bukola Runmonkun ◽  
Omotayo Oluranti Ebong ◽  
Udeme Owunari Georgewill
Keyword(s):  
Diagnostic Test ◽  
Rapid Diagnostic Test ◽  
Malaria Rapid Diagnostic Test ◽  
Test Kit

scholarly journals Rapid Diagnostic Test Kit for Point-of-Care Cerebrospinal Fluid Leak Detection

2019 ◽  
Vol 25 (1) ◽  
pp. 67-74
Author(s):  
Daniel W. Bradbury ◽  
Ashley E. Kita ◽  
Kensuke Hirota ◽  
Maie A. St. John ◽  
Daniel T. Kamei
Keyword(s):  
Cerebrospinal Fluid ◽  
Diagnostic Test ◽  
Rapid Diagnostic Test ◽  
Point Of Care ◽  
Cerebrospinal Fluid Leak ◽  
Leak Detection ◽  
Csf Leak ◽  
Medical Provider ◽  
Test Kit ◽  
Csf Leaks

Cerebrospinal fluid (CSF) leaks can occur when there is communication between the intracranial cavities and the external environment. They are a common and serious complication of numerous procedures in otolaryngology, and if not treated, persistent leaks can increase a patient’s risk of developing life-threatening complications such as meningitis. As it is not uncommon for patients to exhibit increased secretions postoperatively, distinguishing normal secretions from those containing CSF can be difficult. Currently, there are no proven, available tests that allow a medical provider concerned about a CSF leak to inexpensively, rapidly, and noninvasively rule out the presence of a leak. The gold standard laboratory-based test requires that a sample be sent to a tertiary site for analysis, where days to weeks may pass before results return. To address this, our group recently developed a semiquantitative, barcode-style lateral-flow immunoassay (LFA) for the quantification of the beta-trace protein, which has been reported to be an indicator of the presence of CSF leaks. In the work presented here, we created a rapid diagnostic test kit composed of our LFA, a collection swab, dilution buffers, disposable pipettes, and instructions. Validation studies demonstrated excellent predictive capabilities of this kit in distinguishing between clinical specimens containing CSF and those that did not. Our diagnostic kit for CSF leak detection can be operated by an untrained user, does not require any external equipment, and can be performed in approximately 20 min, making it well suited for use at the point of care. This kit has the potential to transform patient outcomes.

scholarly journals An evaluation of the diagnostic performance characteristics of the Yellow Fever IgM immunochromatographic rapid diagnostic test kit from SD Biosensor in Ghana

PLoS ONE ◽  
2022 ◽  
Vol 17 (1) ◽  
pp. e0262312
Author(s):  
Lawrence Henry Ofosu-Appiah ◽  
Dodzi Kofi Amelor ◽  
Bright Ayensu ◽  
Ernest Akyereko ◽  
Nafisah Issah Rabiwu ◽  
...  
Keyword(s):  
Diagnostic Test ◽  
Rapid Diagnostic Test ◽  
Yellow Fever ◽  
Diagnostic Performance ◽  
Yellow Fever Virus ◽  
Point Of Care ◽  
Clinical Signs ◽  
Cross Reactivity ◽  
Performance Characteristics ◽  
Test Kit

Yellow fever is endemic in Ghana and outbreaks occur periodically. The prodromal signs due to Yellow Fever Virus (YFV) infection are non-specific, making clinical signs unreliable as the sole criteria for diagnosis. Accurate laboratory confirmation of suspected yellow fever cases is therefore vital in surveillance programs. Reporting of ELISA IgM testing results by laboratories can delay due to late arrival of samples from the collection sites as well as limited availability of ELISA kits. In this study, the diagnostic performance characteristics of a rapid immunochromatographic Standard Q Yellow Fever IgM test kit (SD Biosensor) was evaluated for the rapid diagnosis of Yellow Fever infection in Ghana. A panel of 275 sera, comprising 81 confirmed YFV positives and 194 negatives were re-tested in this study using the Standard Q Yellow Fever IgM test kit. Using the CDC/WHO Yellow Fever IgM capture ELISA as a benchmark, the sensitivity, specificity and accuracy of the Standard Q Yellow Fever test kit were 96.3%, 97.9% and 97.5%, respectively. The false positivity rate was 5.1% and there was no cross-reactivity when the Standard Q Yellow Fever test kit was tested against dengue, malaria and hepatitis B and C positive samples. In addition, inter-reader variability and invalid rate were both zero. The results indicate that the diagnostic performance of the Standard Q Yellow Fever IgM test kit on serum or plasma is comparable to the serum IgM detection by ELISA and can be used as a point of care rapid diagnostic test kit for YFV infection in endemic areas.

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