Microbial communities responsible for fixation of CO2 revealed by using mcrA, cbbM, cbbL, fthfs, fefe-hydrogenase genes as molecular biomarkers in petroleum reservoirs of different temperatures

2016 ◽  
Vol 114 ◽  
pp. 164-175 ◽  
Author(s):  
Jin-Feng Liu ◽  
Serge Maurice Mbadinga ◽  
Xiao-Bo Sun ◽  
Guang-Chao Yang ◽  
Shi-Zhong Yang ◽  
...  
Keyword(s):  
Microbial Communities ◽  
Petroleum Reservoirs ◽  
Molecular Biomarkers ◽  
Different Temperatures

scholarly journals Microbial communities responsible for fixation of CO<sub>2</sub> revealed by using <i>mcrA</i>, <i>cbbM</i>, <i>cbbL</i>, <i>fthfs</i>, <i>fefe-hydrogenase</i> genes as molecular biomarkers in petroleum reservoirs of different temperatures

2015 ◽  
Vol 12 (2) ◽  
pp. 1875-1906 ◽  
Author(s):  
J.-F. Liu ◽  
S. M. Mbadinga ◽  
X.-B. Sun ◽  
G.-C. Yang ◽  
S.-Z. Yang ◽  
...  
Keyword(s):  
Microbial Communities ◽  
Petroleum Reservoirs ◽  
Oil Reservoirs ◽  
Functional Genes ◽  
Rich Diversity ◽  
New Energy ◽  
Different Temperatures ◽  
In Situ Bioconversion ◽  
High And Low Temperatures

Abstract. Sequestration of CO2 in oil reservoir is one of the feasible options for mitigating atmospheric CO2 building up. The in situ bioconversion of sequestrated CO2 to methane by microorganisms inhabiting oil reservoirs is feasible. To evaluate the potential of in situ microbial fixation and conversion of CO2 into CH4 in oil reservoirs, a comprehensive molecular survey was performed to reveal microbial communities inhabiting four oil reservoirs with different temperatures by analysis of functional genes involved in the biochemical pathways of CO2 fixation and CH4 synthesis (cbbM, cbbL, fthfs, [FeFe]-hydrogenase encoding gene, and mcrA). A rich diversity of these functional genes was found in all the samples with both high and low temperatures and they were affiliated to members of the Proteobacteria (cbbL and cbbM, fthfs), Firmicutes and Actinobacteria (fthfs), uncultured bacteria ([FeFe]-hydrogenase), and Methanomirobiales, Methanobacteriales and Methanosarcinales (mcrA). The predominant methanogens were all identified to be hydrogenotrophic CO2-reducing physiological types. These results showed that functional microbial communities capable of microbial fixation and bioconversion of CO2 into methane inhabit widely in oil reservoirs, which is helpful to microbial recycling of sequestrated CO2 to further new energy in oil reservoirs.

Methanogenesis in sediments from deep lakes at different temperatures (2–70°C)

1997 ◽  
Vol 36 (6-7) ◽  
pp. 57-64 ◽  
Author(s):  
Alla N. Nozhevnikova ◽  
C. Holliger ◽  
A. Ammann ◽  
A. J. B. Zehnder
Keyword(s):  
Organic Matter ◽  
Microbial Communities ◽  
Methane Production ◽  
Low Temperatures ◽  
Main Question ◽  
Deep Lakes ◽  
Temperature Range ◽  
Wide Range ◽  
Thermophilic Conditions ◽  
Different Temperatures

Methanogenic degradation of organic matter occurs in a wide temperature range from psychrophilic to extreme thermophilic conditions. Mesophilic and thermophilic methanogenesis is relatively well investigated, but little is known about low temperature methanogenesis and psychrophilic methanogenic communities. The aim of the present work was to study methanogenesis in a wide range of temperatures with samples from sediments of deep lakes. These sediments may be considered deposits of different types of microorganisms, which are constantly exposed to low temperatures. The main question was how psychrophilic methanogenic microbial communities compare to mesophilic and thermophilic ones. Methanogenesis in a temperature range of 2–70°C was investigated using sediment samples from Baldegger lake (65 m) and Soppen lake (25 m), Switzerland. Methane production from organic matter of sediments occurred at all temperatures tested. An exponential dependence of methane production rate was found between 2 and 30°C. Methanogenesis occurred even at 70°C. At the same time stable methane production from organic matter of sediments was observed at temperatures below 10°C. Methanogenic microbial communities were enriched at different temperatures. The communities enriched at 4–8°C had the highest activity at low temperatures indicating that a specific psychrophilic community exists. Addition of substrates such as cellulose, volatile fatty acids (butyrate, propionate, acetate), methanol and H2/CO2 stimulated methane production at all temperatures. H2/CO2 as well as methanol were directly converted to methane under thermophilic conditions. At low temperatures these substrates were converted to methane by a two-step process. First acetate was formed, followed by methane production from acetate. When acetate concentrations were high, acetoclastic methanogenesis was inhibited at low temperatures. This reaction appears to be one of the “bottle neck” in psychrophilic methanogenesis.

Biodegradation of n-alkanes on oil–seawater interfaces at different temperatures and microbial communities associated with the degradation

2018 ◽  
Vol 29 (2) ◽  
pp. 141-157 ◽  
Author(s):  
Synnøve Lofthus ◽  
Roman Netzer ◽  
Anna S. Lewin ◽  
Tonje M. B. Heggeset ◽  
Tone Haugen ◽  
...  
Keyword(s):  
Microbial Communities ◽  
Different Temperatures

scholarly journals Microbial Communities in Long-Term, Water-Flooded Petroleum Reservoirs with Different in situ Temperatures in the Huabei Oilfield, China

PLoS ONE ◽  
2012 ◽  
Vol 7 (3) ◽  
pp. e33535 ◽  
Author(s):  
Yue-Qin Tang ◽  
Yan Li ◽  
Jie-Yu Zhao ◽  
Chang-Qiao Chi ◽  
Li-Xin Huang ◽  
...  
Keyword(s):  
Microbial Communities ◽  
Petroleum Reservoirs

scholarly journals Differences in microbial community composition between injection and production water samples of water flooding petroleum reservoirs

2015 ◽  
Vol 12 (11) ◽  
pp. 3403-3414 ◽  
Author(s):  
P. K. Gao ◽  
G. Q. Li ◽  
H. M. Tian ◽  
Y. S. Wang ◽  
H. W. Sun ◽  
...  
Keyword(s):  
Microbial Communities ◽  
Water Samples ◽  
High Throughput Sequencing ◽  
Strong Association ◽  
Microbial Community Composition ◽  
Petroleum Reservoirs ◽  
Water Flooding ◽  
Microbial Populations ◽  
Production Wells ◽  
Sandstone Reservoir

Abstract. Microbial communities in injected water are expected to have significant influence on those of reservoir strata in long-term water flooding petroleum reservoirs. To investigate the similarities and differences in microbial communities in injected water and reservoir strata, high-throughput sequencing of microbial partial 16S rRNA of the water samples collected from the wellhead and downhole of injection wells, and from production wells in a homogeneous sandstone reservoir and a heterogeneous conglomerate reservoir were performed. The results indicate that a small number of microbial populations are shared between the water samples from the injection and production wells in the sandstone reservoir, whereas a large number of microbial populations are shared in the conglomerate reservoir. The bacterial and archaeal communities in the reservoir strata have high concentrations, which are similar to those in the injected water. However, microbial population abundance exhibited large differences between the water samples from the injection and production wells. The number of shared populations reflects the influence of microbial communities in injected water on those in reservoir strata to some extent, and show strong association with the unique variation of reservoir environments.

Wellhead Samples of High-Temperature, Low-Permeability Petroleum Reservoirs Reveal the Microbial Communities in Wellbores

2017 ◽  
Vol 31 (5) ◽  
pp. 4866-4874 ◽  
Author(s):  
Zhiyong Song ◽  
Zhi Yao ◽  
Fengmin Zhao ◽  
Gangzheng Sun ◽  
Weiyao Zhu
Keyword(s):  
High Temperature ◽  
Microbial Communities ◽  
Petroleum Reservoirs ◽  
Low Permeability

scholarly journals Stratified microbial communities are highly sensitive towards multiple combined global change factors, revealing antagonistic and synergistic effects

2021 ◽  
Author(s):  
Marcel Suleiman ◽  
Yves Choffat ◽  
Xue Zheng ◽  
Owen Petchey
Keyword(s):  
Global Change ◽  
Microbial Communities ◽  
Multiple Stressors ◽  
Synergistic Effects ◽  
Species Abundance ◽  
Change Factors ◽  
Highly Sensitive ◽  
Different Temperatures ◽  
Global Change Scenarios ◽  
Shed Light

Microbial communities in many ecosystems are facing a broad range of global change scenarios, resulting in microbial changes and possibly regime shifts with unknown ecological consequences. While the influence of single stressors is already described in numerous studies, the effects of multiple stressors working simultaneously are still poorly understood. In this study, we used 240 highly replicable oxic/anoxic aquatic lab micro-ecosystems to understand the influence of four stressors (fertilizer, glyphosate, metal pollution, antibiotics) in all possible combinations at three different temperatures (20 °C, 24 °C, and 28 °C) to shed light into consequences of multiple stressors on different levels of organization, ranging from species abundance to community and ecosystem parameters. Our data reveal that (i) combination of specific stressors can change the biological consequence and direction compared to single stressors in all levels of organisation (ii), effects of stressor combinations are modified by temperature, and (iii) that the number of stressors applied also lead to significant changes. In sum, our study confirmed the need of investigating multiple stressors working simultaneously across different ecological levels of organisation.

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