Effects of three storage conditions on the long-term storage and short-term reactivation performances of anammox granular sludge

Hard cider production in the United States has increased dramatically during the past decade, but there is little information on how harvest and postharvest practices affect the chemistry of the resulting cider, including concentrations of organoleptically important flavanols. For 2 years we assessed fruit, juice, and cider from a total of five apple (Malus ×domestica Borkh.) cultivars in two experiments: sequential harvests and postharvest storage. Different cultivars were used in 2015 and 2016 with the exception of ‘Dabinett’, which was assessed in both years. There were no differences in polyphenol concentrations in cider made from fruit that was harvested on three separate occasions over a 4-week period in either 2015 or 2016. Fruit storage durations and temperatures had little influence on the chemistry when the experiment was conducted in 2015, but polyphenol concentration was greater after storage in the 2016 experiment. In 2016, total polyphenols in ‘Dabinett’ ciders were 51% greater after short-term storage at 10 °C and 67% greater after long-term storage at 1 °C than the control, which was not subjected to a storage treatment. In 2016, total polyphenols in ‘Binet Rouge’ ciders were 67% greater after short-term storage at 10 °C and 94% greater after long-term storage at 1 °C than the control. Although results varied among cultivars and harvest years, storing apples for longer periods of time and at warmer temperatures may be a strategy to increase polyphenol, particularly flavanol, concentrations in hard cider.

Download Full-text

A feasible method to extract DNA from the cambium of high-canopy trees: from harvest to assessment

Acta Amazonica ◽

10.1590/1809-4392202001571 ◽

2020 ◽

Vol 50 (4) ◽

pp. 335-338

Author(s):

Érica MANGARAVITE ◽

Vanessa TERRA ◽

Eric Koiti Okiyama HATTORI ◽

Thaís Carolina da Silva DAL’SASSO ◽

Leonardo Lopes BHERING ◽

...

Keyword(s):

Genomic Dna ◽

Storage Conditions ◽

Microsatellite Genotyping ◽

Short Term ◽

Feasible Method ◽

Long Term Storage ◽

Canopy Trees ◽

Amazonian Forests ◽

Term Storage

ABSTRACT Many tropical trees have high canopies and their leaves are not accessible. Thus, the use of tissue from a more accessible organ (cambium) for DNA extraction may be an alternative for molecular studies. We adapted a feasible methodology for extracting genomic DNA from cambium tissue harvested in the field for the assessment with PCR. We tested three storage conditions (two buffers and a silica gel) and four periods of time after harvest. We used previously described protocols and tested them on three species that occur in Amazonian forests and other biomes: Anadenanthera peregrina var. peregrina, Cedrela fissilis, and Ceiba speciosa. Our protocol obtained suitable PCR-grade genomic DNA for DNA sequencing and microsatellite genotyping. We recommend the use of silica for long-term storage and the buffer with ascorbic acid for short-term storage.

Download Full-text

Measurable Cytokine Concentrations in Pig Seminal Plasma Are Modified by Semen Handling and Storage

Biology ◽

10.3390/biology9090276 ◽

2020 ◽

Vol 9 (9) ◽

pp. 276

Author(s):

Lorena Padilla ◽

Isabel Barranco ◽

Inmaculada Parrilla ◽

Xiomara Lucas ◽

Heriberto Rodriguez-Martinez ◽

...

Keyword(s):

Seminal Plasma ◽

Biological Fluids ◽

Storage Conditions ◽

Reliable Measurement ◽

Short Term ◽

Gm Csf ◽

Long Term Storage ◽

And Storage ◽

Term Storage

Sample handling and storing are critical steps for the reliable measurement of circulating biomolecules in biological fluids. This study evaluates how cytokine measurements in pig seminal plasma (SP) vary depending on semen handling and SP storage. Thirteen cytokines (GM-CSF, IFNγ, IL-1α, IL-1β, IL-1ra, IL-2, IL-4, IL-6, IL-8, IL-10, IL-12, IL-18 and TNFα) were measured using Luminex xMAP® technology in individual seminal plasma (SP) samples (n = 62) from healthy breeding boars. Three separate experiments explored the delay (2 h and 24 h) in SP collection after ejaculation (Experiment 1) and SP storage, either short-term (5 °C, −20 °C and −80 °C for 72 h, Experiment 2) or long-term (at −20 °C and −80 °C for two months, Experiment 3), before analysis. Levels in fresh SP-samples were used as baseline control values. Delays in SP harvesting of up to 24 h did not substantially impact SP cytokine measurements. Some cytokines showed instability in stored SP samples, mainly in long-term storage. Ideally, cytokines in pig SP should be measured in fresh samples harvested within 24 h after ejaculation. If storage of SP is imperative, storage conditions should be adjusted for each cytokine.

Download Full-text

Co-encapsulation of vegetable oils with phenolic antioxidants and evaluation of their oxidative stability under long-term storage conditions

LWT ◽

10.1016/j.lwt.2021.111033 ◽

2021 ◽

Vol 142 ◽

pp. 111033

Author(s):

Lorine Le Priol ◽

Justine Gmur ◽

Aurélien Dagmey ◽

Sandrine Morandat ◽

Karim El Kirat ◽

...

Keyword(s):

Oxidative Stability ◽

Vegetable Oils ◽

Storage Conditions ◽

Phenolic Antioxidants ◽

Long Term Storage ◽

Term Storage

Download Full-text

Recovery of structure and activity of disintegrated aerobic granular sludge after long-term storage: Effect of exogenous N-acyl-homoserine lactones

Chemosphere ◽

10.1016/j.chemosphere.2021.130894 ◽

2021 ◽

pp. 130894

Author(s):

Wei Gao ◽

Yuanchao Hu ◽

Xianhui Jiao ◽

Mingming Gao ◽

Xinhua Wang

Keyword(s):

Granular Sludge ◽

Aerobic Granular Sludge ◽

Storage Effect ◽

Homoserine Lactones ◽

Long Term Storage ◽

Acyl Homoserine Lactones ◽

Structure And Activity ◽

Term Storage

Download Full-text

Comparison of recovery characteristics between AnAOB and AOB-AnAOB granular sludge after long-term storage

The Science of The Total Environment ◽

10.1016/j.scitotenv.2021.149741 ◽

2021 ◽

pp. 149741

Author(s):

Tao Xiang ◽

Hong Liang ◽

Dawen Gao

Keyword(s):

Granular Sludge ◽

Long Term Storage ◽

Term Storage

Download Full-text

Effects of Long-Term Storage on the Detection of Proteins, DNA, and mRNA in Tissue Microarray Slides

Journal of Histochemistry & Cytochemistry ◽

10.1369/0022155411423779 ◽

2011 ◽

Vol 59 (12) ◽

pp. 1113-1121 ◽

Cited By ~ 35

Author(s):

Christina Karlsson ◽

Mats G. Karlsson

Keyword(s):

Gene Copy Number ◽

Tissue Microarrays ◽

Storage Conditions ◽

Gene Copy ◽

Histological Techniques ◽

Long Term Storage ◽

Term Storage ◽

Formalin Fixed

Storage of tissue slides has been claimed to induce dramatically reduced antigen detection particularly for immunohistochemistry (IHC). With tissue microarrays, the necessity to serially cut blocks in order to obtain as much material as possible is obvious. The presumed adverse effect of storage might hamper such an approach. The authors designed an experimental setting consisting of four different storage conditions with storage time of tissue slides of up to 1 year. Detection of proteins, DNA, and mRNA was performed using IHC and in situ hybridization techniques. Slight but significant changes in IHC occurred over time. The most important factor is the primary antibody used: four showed no significant changes, whereas limited decreases in 8 antibodies could be detected by image analysis. Whether the antigen was nuclear or cytoplasmic/membranous did not matter. No major differences between different storage conditions could be shown, but storage at 4C was overall the best procedure. Furthermore, gene copy number aberrations, chromosomal translocations, and the presence of mRNA could be detected on slides stored up to 1 year. In conclusion, in tissues optimally formalin fixed and using modern histological techniques, only minute changes in tissue antigenicity are induced by long-term storage.

Download Full-text

Effects of Tween 80 and Sucrose on Acute Short-Term Stability and Long-Term Storage at −20 C of a Recombinant Hemoglobin

Journal of Pharmaceutical Sciences ◽

10.1021/js980140v ◽

1998 ◽

Vol 87 (9) ◽

pp. 1062-1068 ◽

Cited By ~ 87

Author(s):

Bruce A. Kerwin ◽

Martin C. Heller ◽

Steven H. Levin ◽

Theodore W. Randolph

Keyword(s):

Tween 80 ◽

Short Term ◽

Term Stability ◽

Long Term Storage ◽

Term Storage

Download Full-text

Is adipose tissue suitable for detection of (synthetic) cannabinoids? A comparative study analyzing antemortem and postmortem specimens following pulmonary administration of JWH-210, RCS-4, as well as ∆9-tetrahydrocannabinol to pigs

Archives of Toxicology ◽

10.1007/s00204-020-02843-x ◽

2020 ◽

Vol 94 (10) ◽

pp. 3421-3431

Author(s):

Nadine Schaefer ◽

Frederike Nordmeier ◽

Ann-Katrin Kröll ◽

Christina Körbel ◽

Matthias W. Laschke ◽

...

Keyword(s):

Adipose Tissue ◽

Synthetic Cannabinoids ◽

Standard Addition ◽

Storage Conditions ◽

Long Term Storage ◽

Low Concentrations ◽

Concentration Changes ◽

Term Storage ◽

Plateau Level

Abstract Examining fatal poisonings, chronic exposure may be reflected by the concentration in tissues known for long-term storage of drugs. Δ9-tetrahydrocannabinol (THC) persists in adipose tissue (AT), but sparse data on synthetic cannabinoids (SC) are available. Thus, a controlled pig study evaluating antemortem (AM) disposition and postmortem (PM) concentration changes of the SC 4-ethylnaphthalene-1-yl-(1-pentylindole-3-yl)methanone (JWH-210) and 2-(4-methoxyphenyl)-1-(1-pentyl-indole-3-yl)methanone (RCS-4) as well as THC in AT was performed. The drugs were administered pulmonarily (200 µg/kg body weight) to twelve pigs. Subcutaneous (s.c.) AT specimens were collected after 15 and 30 min and then hourly up to 8 h. At the end, pigs were sacrificed and s.c., perirenal, and dorsal AT specimens were collected. The carcasses were stored at room temperature (RT; n = 6) or 4 °C (n = 6) and specimens were collected after 24, 48, and 72 h. After homogenization in acetonitrile and standard addition, LC–MS/MS was performed. Maximum concentrations were reached 0.5–2 h after administration amounting to 21 ± 13 ng/g (JWH-210), 24 ± 13 ng/g (RCS-4), and 22 ± 20 ng/g (THC) and stayed at a plateau level. Regarding the metabolites, very low concentrations of N-hydroxypentyl-RCS-4 (HO-RCS-4) were detected from 0.5 to 8 h. PM concentrations of parent compounds did not change significantly (p > 0.05) over time under both storage conditions. Concentrations of HO-RCS-4 significantly (p < 0.05) increased in perirenal AT during storage at RT. These results suggest a rapid distribution and persistence in s.c. AT. Furthermore, AT might be resistant to PM redistribution of parent compounds. However, significant PM increases of metabolite concentrations might be considered in perirenal AT.

Download Full-text