Effects of Tween 80 and Sucrose on Acute Short-Term Stability and Long-Term Storage at −20 C of a Recombinant Hemoglobin

1998 ◽  
Vol 87 (9) ◽  
pp. 1062-1068 ◽  
Author(s):  
Bruce A. Kerwin ◽  
Martin C. Heller ◽  
Steven H. Levin ◽  
Theodore W. Randolph
Keyword(s):  
Tween 80 ◽  
Short Term ◽  
Term Stability ◽  
Long Term Storage ◽  
Term Storage

The Weight Change of Various Light-Cured Restorative Materials Stored in Water

2005 ◽  
Vol 6 (2) ◽  
pp. 72-79 ◽  
Author(s):  
Filiz Yalcin
Keyword(s):  
Weight Change ◽  
Glass Ionomer ◽  
Weight Changes ◽  
Short Term ◽  
Composite Cement ◽  
Long Term Storage ◽  
Significant Difference ◽  
Restorative Materials ◽  
Term Storage

Abstract This study investigated weight changes of seven different light-cured composite restorative materials, one polyacid glass ionomer compomer, and one light-cured glass-ionomer cement following short-term and long-term storage in water. Two packable composites, three universal (hybrid) composites, one microglass composite, one polyacid glass ionomer resin composite (compomer), one microhybrid low-viscosity (flowable) composite, and one light cured glass ionomer composite cement were evaluated in this study. The weight changes of these specimens were measured daily (short-term storage), and they were measured after six weeks (long-term storage) using an electronic analytical balance. A significant difference was found in Ionoliner, Dyract AP, Opticor flow, Charisma, and Solitare 2, but no significant difference was found in the others (Filtek Z 250, Filtek P60, TPH Spectrum, and Valux Plus). Weight change showed a tendency to increase with the time of water storage. The greatest weight change occurred in light-cured glass ionomer composite cement (Ionoliner), which is followed in order by the weight changes in Dyract AP, Opticor Flow, Charisma, Solitare 2, Filtek Z250, Filtek P60, TPH Spectrum; Valux Plus had the least amount of change. Citation Keyf F, Yalcin F. The Weight Change of Various Light-Cured Restorative Materials Stored in Water. J Contemp Dent Pract 2005 May;(6)2:072-079.

scholarly journals Long-Term Nanoleakage Depth and Pattern of Cervical Restorations Bonded With Different Adhesives

2012 ◽  
Vol 37 (1) ◽  
pp. 45-53 ◽  
Author(s):  
EH Mobarak ◽  
LE Daifalla
Keyword(s):  
Clinical Relevance ◽  
Adhesive System ◽  
Single Step ◽  
Short Term ◽  
Long Term Storage ◽  
Term Storage

Clinical Relevance A mild acetone-based single-step self-etched adhesive system may reveal less nanoleakage in the short-term interval, but unfortunately, this was not sustained after long-term storage.

scholarly journals Management of Apple Maturity and Postharvest Storage Conditions to Increase Polyphenols in Cider

HortScience ◽  
2019 ◽  
Vol 54 (1) ◽  
pp. 143-148 ◽  
Author(s):  
Brianna L. Ewing ◽  
Gregory M. Peck ◽  
Sihui Ma ◽  
Andrew P. Neilson ◽  
Amanda C. Stewart
Keyword(s):  
The United States ◽  
Storage Conditions ◽  
Short Term ◽  
Postharvest Storage ◽  
Total Polyphenols ◽  
Fruit Storage ◽  
Long Term Storage ◽  
Short Term Storage ◽  
Term Storage

Hard cider production in the United States has increased dramatically during the past decade, but there is little information on how harvest and postharvest practices affect the chemistry of the resulting cider, including concentrations of organoleptically important flavanols. For 2 years we assessed fruit, juice, and cider from a total of five apple (Malus ×domestica Borkh.) cultivars in two experiments: sequential harvests and postharvest storage. Different cultivars were used in 2015 and 2016 with the exception of ‘Dabinett’, which was assessed in both years. There were no differences in polyphenol concentrations in cider made from fruit that was harvested on three separate occasions over a 4-week period in either 2015 or 2016. Fruit storage durations and temperatures had little influence on the chemistry when the experiment was conducted in 2015, but polyphenol concentration was greater after storage in the 2016 experiment. In 2016, total polyphenols in ‘Dabinett’ ciders were 51% greater after short-term storage at 10 °C and 67% greater after long-term storage at 1 °C than the control, which was not subjected to a storage treatment. In 2016, total polyphenols in ‘Binet Rouge’ ciders were 67% greater after short-term storage at 10 °C and 94% greater after long-term storage at 1 °C than the control. Although results varied among cultivars and harvest years, storing apples for longer periods of time and at warmer temperatures may be a strategy to increase polyphenol, particularly flavanol, concentrations in hard cider.

scholarly journals Integrating Whole Blood Transcriptomic Collection Procedures Into the Current Anti-Doping Testing System, Including Long-Term Storage and Re-Testing of Anti-Doping Samples

2021 ◽  
Vol 8 ◽  
Author(s):  
Giscard Lima ◽  
Alexander Kolliari-Turner ◽  
Fernanda Rossell Malinsky ◽  
Fergus M. Guppy ◽  
Renan Paulo Martin ◽  
...  
Keyword(s):  
Whole Blood ◽  
Differentially Expressed ◽  
Rna Seq ◽  
Short Term ◽  
Long Term Storage ◽  
Short Term Storage ◽  
Short And Long Term ◽  
Rna Preservation ◽  
Term Storage

Introduction: Recombinant human erythropoietin (rHuEPO) administration studies involving transcriptomic approaches have demonstrated a gene expression signature that could aid blood doping detection. However, current anti-doping testing does not involve collecting whole blood into tubes with RNA preservative. This study investigated if whole blood in long-term storage and whole blood left over from standard hematological testing in short-term storage could be used for transcriptomic analysis despite lacking RNA preservation.Methods: Whole blood samples were collected from twelve and fourteen healthy nonathletic males, for long-term and short-term storage experiments. Long-term storage involved whole blood collected into Tempus™ tubes and K2EDTA tubes and subjected to long-term (i.e., ‒80°C) storage and RNA extracted. Short-term storage involved whole blood collected into K2EDTA tubes and stored at 4°C for 6‒48 h and then incubated at room temperature for 1 and 2 h prior to addition of RNA preservative. RNA quantity, purity, and integrity were analyzed in addition to RNA-Seq using the MGI DNBSEQ-G400 on RNA from both the short- and long-term storage studies. Genes presenting a fold change (FC) of >1.1 or < ‒1.1 with p ≤ 0.05 for each comparison were considered differentially expressed. Microarray analysis using the Affymetrix GeneChip® Human Transcriptome 2.0 Array was additionally conducted on RNA from the short-term study with a false discovery ratio (FDR) of ≤0.05 and an FC of >1.1 or < ‒1.1 applied to identify differentially expressed genes.Results: RNA quantity, purity, and integrity from whole blood subjected to short- and long-term storage were sufficient for gene expression analysis. Long-term storage: when comparing blood tubes with and without RNA preservation 4,058 transcripts (6% of coding and non-coding transcripts) were differentially expressed using microarray and 658 genes (3.4% of mapped genes) were differentially expressed using RNA-Seq. Short-term storage: mean RNA integrity and yield were not significantly different at any of the time points. RNA-Seq analysis revealed a very small number of differentially expressed genes (70 or 1.37% of mapped genes) when comparing samples stored between 6 and 48 h without RNA preservative. None of the genes previously identified in rHuEPO administration studies were differently expressed in either long- or short-term storage experiments.Conclusion: RNA quantity, purity, and integrity were not significantly compromised from short- or long-term storage in blood storage tubes lacking RNA stabilization, indicating that transcriptomic analysis could be conducted using anti-doping samples collected or biobanked without RNA preservation.

scholarly journals GENTLE CABBAGE HARVESTING

2020 ◽  
Vol 15 (2) ◽  
pp. 72-76
Author(s):  
Sergey Alatyrev ◽  
Irina Kruchinkina ◽  
Aleksey Alatyrev
Keyword(s):  
Product Quality ◽  
Technological Process ◽  
Mechanical Damage ◽  
Severely Injured ◽  
Combine Operation ◽  
Medium Term ◽  
Short Term ◽  
Long Term Storage ◽  
Term Storage

When harvesting cabbage by machine, the heads of cabbage are severely injured. Mechanically damaged heads of cabbage are poorly stored. Therefore, the machine technology of cabbage harvesting should provide protection of heads of cabbage from mechanical damage. The purpose of the research is to study the qualitative indicators of the operation of a multivariate cabbage harvester with various harvesting technological schemes. The considered technological schemes for harvesting cabbage include: careful shipment of heads in bulk of a universal vehicle (Scheme 1); careful point shipment of heads of cabbage into containers installed in the back of a vehicle (diagram 2); shipment of heads of cabbage on a flexible flooring installed in the vehicle, with their subsequent careful transfer to containers manually (Scheme 3); stowing heads of cabbage into containers on an accompanying trailer manually (Scheme 4). During the production check, under conditions typical for the main regions of mass marketable production of cabbage, the multivariate cabbage harvester steadily performed the technological process. At the same time, its quality indicators met the established agrotechnical requirements. Combine operation according to schemes 3 and 4 ensured the preservation of product quality to the maximum extent: damage to heads of cabbage did not exceed 5 ... 6%, the completeness of cabbage leaves removal was 95 ... 100%. Therefore, its use according to schemes 1 and 2 is recommended mainly when harvesting cabbage for short-term and medium-term storage, according to schemes 3 and 4 - when harvesting heads of cabbage intended for long-term storage

scholarly journals Age of Red Cells for Transfusion and Outcomes in Patients with ARDS

2022 ◽  
Vol 11 (1) ◽  
pp. 245
Author(s):  
Jan A. Graw ◽  
Victoria Bünger ◽  
Lorenz A. Materne ◽  
Alexander Krannich ◽  
Felix Balzer ◽  
...  
Keyword(s):  
Critically Ill ◽  
Critically Ill Patients ◽  
Hazard Ratio ◽  
Short Term ◽  
Long Term Storage ◽  
Short Term Storage ◽  
Successful Weaning ◽  
The Mean ◽  
Term Storage

Packed red blood cells (PRBCs), stored for prolonged intervals, might contribute to adverse clinical outcomes in critically ill patients. In this study, short-term outcome after transfusion of PRBCs of two storage duration periods was analyzed in patients with Acute Respiratory Distress Syndrome (ARDS). Patients who received transfusions of PRBCs were identified from a cohort of 1044 ARDS patients. Patients were grouped according to the mean storage age of all transfused units. Patients transfused with PRBCs of a mean storage age ≤ 28 days were compared to patients transfused with PRBCs of a mean storage age > 28 days. The primary endpoint was 28-day mortality. Secondary endpoints included failure-free days composites. Two hundred and eighty-three patients were eligible for analysis. Patients in the short-term storage group had similar baseline characteristics and received a similar amount of PRBC units compared with patients in the long-term storage group (five units (IQR, 3–10) vs. four units (2–8), p = 0.14). The mean storage age in the short-term storage group was 20 (±5.4) days compared with 32 (±3.1) days in the long-term storage group (mean difference 12 days (95%-CI, 11–13)). There was no difference in 28-day mortality between the short-term storage group compared with the long-term storage group (hazard ratio, 1.36 (95%-CI, 0.84–2.21), p = 0.21). While there were no differences in ventilator-free, sedation-free, and vasopressor-free days composites, patients in the long-term storage group compared with patients in the short-term storage group had a 75% lower chance for successful weaning from renal replacement therapy (RRT) within 28 days after ARDS onset (subdistribution hazard ratio, 0.24 (95%-CI, 0.1–0.55), p < 0.001). Further analysis indicated that even a single PRBC unit stored for more than 28 days decreased the chance for successful weaning from RRT. Prolonged storage of PRBCs was not associated with a higher mortality in adults with ARDS. However, transfusion of long-term stored PRBCs was associated with prolonged dependence of RRT in critically ill patients with an ARDS.

An optically clear hypercholesterolemic hypertriglyceridemic quality-control material prepared from animal lipid sources.

1981 ◽  
Vol 27 (3) ◽  
pp. 468-471 ◽  
Author(s):  
G J Proksch ◽  
D P Bonderman
Keyword(s):  
Accidental Exposure ◽  
Quality Control Material ◽  
Short Term ◽  
Porcine Serum ◽  
Long Term Storage ◽  
Control Serum ◽  
Egg Yolks ◽  
Triton X ◽  
Term Storage

Abstract A hyperlipidemic control serum can be simple prepared from animal lipid sources. Beta- and pre-beta-lipoproteins containing cholesterol and triglyceride are removed from porcine serum by treatment with dextran sulfate and calcium ions. A triglyceride-rich fraction containing only trace amounts of cholesterol is isolated from chicken egg-yolks. The two fractions are then combined in 40 mmol/L sodium bicarbonate to give the desired values for cholesterol and triglyceride. The preparation is stabilized against surface denaturation during long-term storage at 5 degrees C perhaps for as long as two years, by adding 0.25 g of Triton X-100 surfactant per liter, and against an accidental exposure to short-term freezing by adding 10 g of sucrose per liter. We used this solution as a diluent to reconstitute lyophilized bovine serum. The resulting product, having been prepared from only animal sources, is free of hepatitis-associated constituents, and is remarkably clear, homogeneous, and stable. Results obtained with it are precise.

scholarly journals Integrated Modelling of Runoff, Alkalinity, and pH on a Daily Basis

1985 ◽  
Vol 16 (2) ◽  
pp. 89-104 ◽  
Author(s):  
S. Bergström ◽  
B. Carlsson ◽  
G. Sandberg ◽  
L. Maxe
Keyword(s):  
River System ◽  
Daily Basis ◽  
Integrated Modelling ◽  
Running Waters ◽  
Short Term ◽  
Long Term Storage ◽  
Semi Empirical ◽  
Term Storage

Based on the experience from runoff and groundwater recharge simulation a model system has been developed for terrestrial, hydrochemical, and hydrological simulations. The system emphasizes the role of temporary or long term storage in the aquifers of a basin and, separately, accounts for each rainfall or snowmelt event from its entrance into the ground until mixing in the river system. The model is primarily intended for simulation of natural short term variations in alkalinity and pH in running waters. The hydrochemical processes are modelled in a semi-empirical way without assumption of complete hydrochemichal mass-balance. In the paper a brief hydrochemical background is given, and a model with two alternative hydrochemical sub-structures is described. Examples of daily simulations of runoff alkalinity and pH from three different basins are given.

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