Lactational changes in phospholipid classes and molecular species concentration in human milk

SUMMARYThis study is concerned with the influence of testosterone on the phospholipid class and the phosphatidylcholine molecular species composition of various fractions obtained from the blood of Plasmodium chabaudi-infected mice. Blood plasma, infected erythrocytes, isolated parasites and erythrocyte membranes isolated from both non-infected and infected erythrocytes in the form of ghosts were analysed. In general, the phospholipid classes remained unaffected, while the phosphatidylcholine (PC) molecular species composition showed differences after testosterone treatment. In infected erythrocytes, there was a decrease in 16:0/20:4-PC and 18:0/20:4-PC and an increase in 16:0/18:2(16:0/20:3)-PC. The decrease of 16:0/20:4-PC was exclusively confined to parasites. The rise in 16:0/18:2(16:0/20: 3)-PC and the diminution of 18:0/20:4-PC occurred in the erythrocyte membrane of both infected ghosts and non-infected ghosts as well as in the blood plasma. It is suggested that these changes occur primarily in the plasma thereby influencing the erythrocyte membranes. The decrease in 16:0/20:4-PC supports the view of the independence of the parasite from the biosynthetic lipid pathways of its host cell.

Download Full-text

Optimization and validation of HRLC-MS method to identify and quantify triacylglycerol molecular species in human milk

Analytical Methods ◽

10.1039/c5ay00591d ◽

2015 ◽

Vol 7 (10) ◽

pp. 4362-4370 ◽

Cited By ~ 9

Author(s):

Kyeong-Mu Kim ◽

Tae-Sik Park ◽

Soon-Mi Shim

Keyword(s):

Human Milk ◽

Molecular Species ◽

Milk Fat ◽

Infant Formulas ◽

Triacylglycerol Molecular Species ◽

Human Milk Fat

Study of the determination of triacylglycerols (TAG) molecular species in human milk is necessary for understanding the absorption of human milk fat as well as designing milk fats for infant formulas.

Download Full-text

Oestradiol-induced changes in the composition of phospholipid classes of quail oviduct: specific replacement of arachidonic acid by docosahexaenoic acid in alkenylacyl-glycerophosphoethanolamine

Biochemical Journal ◽

10.1042/bj3010361 ◽

1994 ◽

Vol 301 (2) ◽

pp. 361-366 ◽

Cited By ~ 5

Author(s):

B E Felouati ◽

J F Pageaux ◽

J M Fayard ◽

M Lagarde ◽

C Laugier

Keyword(s):

Cell Proliferation ◽

Arachidonic Acid ◽

Molecular Species ◽

Phospholipid Composition ◽

Oestrogen Treatment ◽

Ethanolamine Glycerophospholipids ◽

Striking Result ◽

Choline Glycerophospholipids ◽

Phospholipid Classes ◽

Induced Changes

The phospholipid composition and the molecular species of the major subclasses of ethanolamine and choline glycerophospholipids were determined during the natural or oestradiol-induced development of the quail oviduct. The phospholipid concentration increased significantly during oviduct development, and the proportion of ethanolamine glycerophospholipids (EPL) remained constant while that of choline glycerophospholipids increased. The immature oviduct contained the majority of its endogenous arachidonic acid mass (71%) in EPL, mainly in alkenylacyl-glycerophosphoethanolamine (alkenylacyl-GPE) (49% of the total). Oestrogen treatment induced the depletion of 20:4,n-6 specifically from this pool, which indicates the biological importance of 20:4,n-6 molecular species in alkenylacyl-GPE as substrates for the oviduct phospholipases activated by oestradiol, and suggests that this EPL subclass is involved in the oestrogen-induced cell proliferation. Another striking result was the marked increase in 22:6,n-3 EPL molecular species following the oestradiol treatment and more particularly the strict substitution of 20:4,n-6 by 22:6,n-3 in alkenylacyl-GPE. We speculate that alkenylacyl-GPE molecular species containing 22:6,n-3 may participate in the arrest of oestrogen-induced proliferation.

Download Full-text