Testosterone-induced changes in phosphatidylcholine molecular species composition of Plasmodium chabaudi-infected erythrocytes

Parasitology ◽  
1993 ◽  
Vol 107 (5) ◽  
pp. 465-469
Author(s):  
S. Fiebig ◽  
A. P. Simões ◽  
F. Wunderlich ◽  
J. A. F. Op Den Kamp
Keyword(s):  
Species Composition ◽  
Blood Plasma ◽  
Molecular Species ◽  
Phospholipid Class ◽  
Erythrocyte Membranes ◽  
Plasmodium Chabaudi ◽  
Molecular Species Composition ◽  
Testosterone Treatment ◽  
Phospholipid Classes ◽  
Induced Changes

SUMMARYThis study is concerned with the influence of testosterone on the phospholipid class and the phosphatidylcholine molecular species composition of various fractions obtained from the blood of Plasmodium chabaudi-infected mice. Blood plasma, infected erythrocytes, isolated parasites and erythrocyte membranes isolated from both non-infected and infected erythrocytes in the form of ghosts were analysed. In general, the phospholipid classes remained unaffected, while the phosphatidylcholine (PC) molecular species composition showed differences after testosterone treatment. In infected erythrocytes, there was a decrease in 16:0/20:4-PC and 18:0/20:4-PC and an increase in 16:0/18:2(16:0/20:3)-PC. The decrease of 16:0/20:4-PC was exclusively confined to parasites. The rise in 16:0/18:2(16:0/20: 3)-PC and the diminution of 18:0/20:4-PC occurred in the erythrocyte membrane of both infected ghosts and non-infected ghosts as well as in the blood plasma. It is suggested that these changes occur primarily in the plasma thereby influencing the erythrocyte membranes. The decrease in 16:0/20:4-PC supports the view of the independence of the parasite from the biosynthetic lipid pathways of its host cell.

Molecular species composition of glycolipids from Sprirulina platensis

2002 ◽  
Vol 77 (1) ◽  
pp. 9-13 ◽  
Author(s):  
Changhu Xue ◽  
Yaqin Hu ◽  
Hiroaki Saito ◽  
Zhaohui Zhang ◽  
Zhaojie Li ◽  
...  
Keyword(s):  
Species Composition ◽  
Molecular Species ◽  
Molecular Species Composition

scholarly journals The contributions of biosynthesis and acyl chain remodelling to the molecular species profile of phosphatidylcholine in yeast

2005 ◽  
Vol 33 (5) ◽  
pp. 1146-1149 ◽  
Author(s):  
H.A. Boumann ◽  
A.I.P.M. de Kroon
Keyword(s):  
Stable Isotope ◽  
Species Composition ◽  
Molecular Species ◽  
Acyl Chain ◽  
Membrane Lipid ◽  
Molecular Species Composition ◽  
Stable Isotope Labelling ◽  
Membrane Function ◽  
Isotope Labelling ◽  
Acyl Chains

Phosphatidylcholine (PC) is a very abundant membrane lipid in most eukaryotes, including yeast. The molecular species profile of PC, i.e. the ensemble of PC molecules with acyl chains differing in number of carbon atoms and double bonds, is important for membrane function. Pathways of PC synthesis and turnover maintain PC homoeostasis and determine the molecular species profile of PC. Studies addressing the processes involved in establishing the molecular species composition of PC in yeast using stable isotope labelling combined with detection by MS are reviewed.

scholarly journals Oestradiol-induced changes in the composition of phospholipid classes of quail oviduct: specific replacement of arachidonic acid by docosahexaenoic acid in alkenylacyl-glycerophosphoethanolamine

1994 ◽  
Vol 301 (2) ◽  
pp. 361-366 ◽  
Author(s):  
B E Felouati ◽  
J F Pageaux ◽  
J M Fayard ◽  
M Lagarde ◽  
C Laugier
Keyword(s):  
Cell Proliferation ◽  
Arachidonic Acid ◽  
Molecular Species ◽  
Phospholipid Composition ◽  
Oestrogen Treatment ◽  
Ethanolamine Glycerophospholipids ◽  
Striking Result ◽  
Choline Glycerophospholipids ◽  
Phospholipid Classes ◽  
Induced Changes

The phospholipid composition and the molecular species of the major subclasses of ethanolamine and choline glycerophospholipids were determined during the natural or oestradiol-induced development of the quail oviduct. The phospholipid concentration increased significantly during oviduct development, and the proportion of ethanolamine glycerophospholipids (EPL) remained constant while that of choline glycerophospholipids increased. The immature oviduct contained the majority of its endogenous arachidonic acid mass (71%) in EPL, mainly in alkenylacyl-glycerophosphoethanolamine (alkenylacyl-GPE) (49% of the total). Oestrogen treatment induced the depletion of 20:4,n-6 specifically from this pool, which indicates the biological importance of 20:4,n-6 molecular species in alkenylacyl-GPE as substrates for the oviduct phospholipases activated by oestradiol, and suggests that this EPL subclass is involved in the oestrogen-induced cell proliferation. Another striking result was the marked increase in 22:6,n-3 EPL molecular species following the oestradiol treatment and more particularly the strict substitution of 20:4,n-6 by 22:6,n-3 in alkenylacyl-GPE. We speculate that alkenylacyl-GPE molecular species containing 22:6,n-3 may participate in the arrest of oestrogen-induced proliferation.

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