CRISPR/Cas12a-assisted rapid identification of key beer spoilage bacteria

2021 ◽  
pp. 102854
Author(s):  
Qingzhou Meng ◽  
Hongmei Yang ◽  
Guiquan Zhang ◽  
Wenjun Sun ◽  
Peixiang Ma ◽  
...  
Keyword(s):  
Rapid Identification ◽  
Spoilage Bacteria ◽  
Beer Spoilage

Application of Rep-PCR fingerprint in rapid identification of beer-spoilage bacteria

2006 ◽  
Vol 22 (6) ◽  
pp. 1013-1021 ◽  
Author(s):  
L ZHU ◽  
F ZHENG ◽  
Y ZHAO ◽  
X XING ◽  
Q LI ◽  
...  
Keyword(s):  
Rapid Identification ◽  
Spoilage Bacteria ◽  
Beer Spoilage

Identification of novel horA-harbouring bacteria capable of spoiling beer

2008 ◽  
Vol 54 (4) ◽  
pp. 321-325 ◽  
Author(s):  
Monique Haakensen ◽  
Barry Ziola
Keyword(s):  
Drug Resistance ◽  
Bacillus Cereus ◽  
Bacillus Licheniformis ◽  
Staphylococcus Epidermidis ◽  
Multi Drug Resistance ◽  
Bacterial Isolates ◽  
First Finding ◽  
Spoilage Bacteria ◽  
Beer Spoilage ◽  
Respective Species

An ATP-binding cassette (ABC) multi-drug resistance (MDR) gene was found in 4 Gram-positive bacterial isolates of environmental origin and found capable of spoiling beer. The bacteria isolated were Bacillus cereus , Bacillus licheniformis , Paenibacillus humicus , and Staphylococcus epidermidis ; all of which were previously unappreciated as beer-spoilage bacteria. The MDR gene found in these bacteria has less than 37% similarity to known ABC MDR proteins described for Bacillus and Staphylococcus , and this is the first finding of an ABC MDR gene in the genus Paenibacillus . The sequenced region of the gene was translated and compared phylogenetically with the closest GenBank matches of the respective species and the closest GenBank matches overall. The ABC MDR proteins from these isolates were found to cluster among known sequences of HorA, sharing 99.5% identity within the sequenced region. In the beer-spoilage-associated genera Lactobacillus and Pediococcus , the presence of the MDR gene horA correlates with the ability to grow in beer. As the unique horA-harbouring isolates described here are capable of growing in beer, it is likely that the presence of the horA gene likewise confers hop resistance to these organisms.

BEER-SPOILAGE BACTERIA AND THEIR CONTROL WITH A PHOSPHORIC ACID – AMMONIUM PERSULFATE WASH

1965 ◽  
Vol 11 (2) ◽  
pp. 309-318 ◽  
Author(s):  
Stanley Bah ◽  
W. E. McKeen
Keyword(s):  
Phosphoric Acid ◽  
Treatment Time ◽  
Ammonium Persulfate ◽  
Acetobacter Aceti ◽  
Lager Yeast ◽  
Great Saving ◽  
Spoilage Bacteria ◽  
Plant Equipment ◽  
Beer Spoilage ◽  
The One

Flavobacterium proteus, Acetobacter aceti, Acetobacter capsulatum, Pediococcus cerevisiae, and Lactobacillus pastorianus were isolated from contaminated yeast in certain Canadian breweries. F. proteus was most prevalent in ale yeast, while L. pastorianus and P. cerevisiae were most prevalent in lager yeast but the plant also affected the prevalence of the species.A phosphoric acid – ammonium persulfate wash was developed which has proved very effective in the breweries in which it has been used since 1960. Treatment time is very short (2 hours) and consequently a great saving in plant equipment and labor has resulted.The fermentative ability and viability of Saccharomyces cerevisiae is not affected nor is it killed even when it is treated with a phosphoric acid – ammonium persulfate wash that is six times as strong as the one recommended for use in the brewery.

Serogroups of the beer spoilage bacterium Megasphaera cerevisiae correlate with the molecular weight of the major EDTA-extractable surface protein

2000 ◽  
Vol 46 (2) ◽  
pp. 95-100 ◽  
Author(s):  
Barry Ziola ◽  
Lori Gee ◽  
Nancy N Berg ◽  
Sun Y Lee
Keyword(s):  
Ethylenediaminetetraacetic Acid ◽  
Polyacrylamide Gel Electrophoresis ◽  
Sodium Dodecyl ◽  
Surface Protein ◽  
Cross Reactivity ◽  
Surface Reactivity ◽  
Surface Proteins ◽  
Bacterial Surface ◽  
Spoilage Bacteria ◽  
Beer Spoilage

Megasphaera cerevisiae is a Gram-negative obligate anaerobe that causes turbidity and off-flavour and aroma in beer. Seven isolates of M. cerevisiae were obtained worldwide, and their extractable surface antigens were focused upon to determine if there is more than one serogroup of this bacterium. Sodium dodecyl sulphate polyacrylamide gel electrophoresis of ethylenediaminetetraacetic acid (EDTA) bacterial extracts revealed a predominant protein with apparent molecular weights of 46 000, 45 000, and 43 000 for three, two, and two isolates, respectively. When mouse antiserum generated against any of the EDTA extracts was reacted with denatured bacterial proteins in immunoblots, all bacterial isolates exhibited extensive cross-reactivity involving three antigens, one being the major EDTA-extractable protein. In contrast, when the sera were tested for surface reactivity with intact bacteria, three cross-reactivity groups were observed, with the groups individually comprised of bacteria having the same size major EDTA-extractable surface protein. When BALB/c mice immunized with a bacterium from each of the three serogroups were used for monoclonal antibody (Mab) hybridoma production, bacterial surface-reactive Mabs were obtained whose reactivities parallel the three polyclonal antibody-defined serogroups. Through combining these surface-reactive Mabs, it will be possible to rapidly detect and identify beer contamination by M. cerevisiae belonging to any serogroup. Key words: beer spoilage bacteria, Megasphaera cerevisiae, monoclonal antibodies, surface proteins, serogroups.

Isolation of a Virulent Lactobacillus brevis Phage and Its Application in the Control of Beer Spoilage

2011 ◽  
Vol 74 (12) ◽  
pp. 2157-2161 ◽  
Author(s):  
THERESE DEASY ◽  
JENNIFER MAHONY ◽  
HORST NEVE ◽  
KNUT J. HELLER ◽  
DOUWE VAN SINDEREN
Keyword(s):  
Electron Microscopy ◽  
Lactobacillus Plantarum ◽  
Bacterial Contamination ◽  
Lactobacillus Brevis ◽  
Virulent Phage ◽  
Spoilage Bacteria ◽  
Food And Beverage ◽  
Beer Spoilage ◽  
Bacterial Spoilage

Beer quality can be compromised by the growth of certain lactobacilli, in particular Lactobacillus brevis and Lactobacillus plantarum, and various strategies have been used to control such bacterial spoilage. Biocontrol by means of bacteriophage is a reemerging approach for the suppression of spoilage bacteria in food and beverage matrices. A virulent phage capable of infecting L. brevis beer-spoilage strains was isolated and morphologically assessed by electron microscopy. The myophage SA-C12 was shown to be stable in beer and capable of controlling the growth of its host, L. brevis strain 56, in commercial beer. The results of this study indicate that bacteriophage-based treatments may be used as an alternative and natural strategy for the control of bacterial contamination of beer.

Epitope mapping of monoclonal antibodies specific for the directly cross-linkedmeso-diaminopimelic acid peptidoglycan found in the anaerobic beer spoilage bacteriumPectinatus cerevisiiphilus

1999 ◽  
Vol 45 (9) ◽  
pp. 779-785 ◽  
Author(s):  
Barry Ziola ◽  
Sheryl L Gares ◽  
Brandene Lorrain ◽  
Lori Gee ◽  
W M Ingledew ◽  
...  
Keyword(s):  
Monoclonal Antibodies ◽  
Outer Membrane ◽  
Epitope Mapping ◽  
Sodium Dodecyl ◽  
Diaminopimelic Acid ◽  
Gram Negative Bacteria ◽  
Gram Negative ◽  
Spoilage Bacteria ◽  
Beer Spoilage ◽  
Bacterial Outer Membrane

Nineteen monoclonal antibodies (Mabs) were isolated based on reactivity with disrupted Pectinatus cerevisiiphilus cells. All of the Mabs reacted with cells from which the outer membrane had been stripped by incubation with sodium dodecyl sulphate, suggesting the peptidoglycan (PG) layer was involved in binding. Mab reactivity with purified PG confirmed this. Epitope mapping revealed the Mabs in total recognize four binding sites on the PG. Mabs specific for each of the four sites also bound strongly to disrupted Pectinatus frisingensis, Selenomonas lacticifix, Zymophilus paucivorans, and Zymophilus raffinosivorans cells, but weakly to disrupted Megasphaera cerevisiae cells. No antibody reactivity was seen with disrupted cells of 11 other species of Gram-negative bacteria. These results confirm that a common PG structure is used by several species of anaerobic Gram-negative beer spoilage bacteria. These results also indicate that PG-specific Mabs can be used to rapidly detect a range of anaerobic Gram-negative beer spoilage bacteria, provided the bacterial outer membrane is first removed to allow antibody binding.Key words: beer spoilage, epitope mapping, monoclonal antibodies, Pectinatus, peptidoglycan.

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