Antifungal activity of 1-methylcyclopropene (1-MCP) against anthracnose ( Colletotrichum gloeosporioides ) in postharvest mango fruit and its possible mechanisms of action

Silver particles were prepared by dewetting Ag films coated on glass using a fiber laser. The size of the particles was controlled in the range of 92 nm–1.2 μm by adjusting the thickness of the Ag film. The structural properties and surface roughness of the particles were evaluated by means of scanning electron microscopy. In addition, the antifungal activity of the Ag particles was examined using spore suspensions of Colletotrichum gloeosporioides. It is shown that particles with a size of 1.2 μm achieved 100% inhibition of conidia growth of C. gloeosporioides after a contact time of just 5 min. Furthermore, the smaller particles also achieved good antifungal activity given a longer contact time. Similar results were observed for spore germination and pathogenicity tests performed on mango fruit and leaves. Overall, the results confirm that Ag particles have an excellent antifungal effect on C. gloeosporioides.

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Antifungal Activity of Propyl Disulfide from Neem (Azadirachta indica) in Vapor and Agar Diffusion Assays against Anthracnose Pathogens (Colletotrichum gloeosporioides and Colletotrichum acutatum) in Mango Fruit

Microorganisms ◽

10.3390/microorganisms9040839 ◽

2021 ◽

Vol 9 (4) ◽

pp. 839

Author(s):

Muhammad Rafiullah Khan ◽

Vanee Chonhenchob ◽

Chongxing Huang ◽

Panitee Suwanamornlert

Keyword(s):

Antifungal Activity ◽

Mycelial Growth ◽

Colletotrichum Gloeosporioides ◽

Colletotrichum Acutatum ◽

Model Parameters ◽

Pathogenicity Test ◽

Agar Diffusion ◽

Wide Range ◽

Significant Difference ◽

Diffusion Assay

Microorganisms causing anthracnose diseases have a medium to a high level of resistance to the existing fungicides. This study aimed to investigate neem plant extract (propyl disulfide, PD) as an alternative to the current fungicides against mango’s anthracnose. Microorganisms were isolated from decayed mango and identified as Colletotrichum gloeosporioides and Colletotrichum acutatum. Next, a pathogenicity test was conducted and after fulfilling Koch’s postulates, fungi were reisolated from these symptomatic fruits and we thus obtained pure cultures. Then, different concentrations of PD were used against these fungi in vapor and agar diffusion assays. Ethanol and distilled water were served as control treatments. PD significantly (p ≤ 0.05) inhibited more of the mycelial growth of these fungi than both controls. The antifungal activity of PD increased with increasing concentrations. The vapor diffusion assay was more effective in inhibiting the mycelial growth of these fungi than the agar diffusion assay. A good fit (R2, 0.950) of the experimental data in the Gompertz growth model and a significant difference in the model parameters, i.e., lag phase (λ), stationary phase (A) and mycelial growth rate, further showed the antifungal efficacy of PD. Therefore, PD could be the best antimicrobial compound against a wide range of microorganisms.

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Effect of hot water treatment on development of anthracnose (Colletotrichum gloeosporioides) and quality of mango fruit at Jimma southwest Ethiopia

Archives of Phytopathology and Plant Protection ◽

10.1080/03235408.2017.1311441 ◽

2017 ◽

Vol 50 (7-8) ◽

pp. 303-316

Author(s):

Agurash Seid ◽

Debrie Tasew ◽

Binyam Tsedaley

Keyword(s):

Water Treatment ◽

Colletotrichum Gloeosporioides ◽

Hot Water ◽

Hot Water Treatment ◽

Mango Fruit ◽

Southwest Ethiopia

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Dinactin from a new producer, Streptomyces badius gz-8, and its antifungal activity against the rubber anthracnose fungus Colletotrichum gloeosporioides

Microbiological Research ◽

10.1016/j.micres.2020.126548 ◽

2020 ◽

Vol 240 ◽

pp. 126548

Author(s):

Kai Zhang ◽

Liushuang Gu ◽

Yuefeng Zhang ◽

Zhiqiang Liu ◽

Xiaoyu Li

Keyword(s):

Antifungal Activity ◽

Colletotrichum Gloeosporioides

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Physicochemical characterization, antioxidant and antifungal activity of three stingless bee pollen aggregate (Apidae: Meliponini) from Soconusco, Chiapas

Revista Mexicana de Fitopatología Mexican Journal of Phytopathology ◽

10.18781/r.mex.fit.2009-4 ◽

2020 ◽

Vol 39 (1) ◽

Author(s):

Víctor Albores-Flores ◽

Erick Saavedra-Camacho ◽

José Alfonso López-García ◽

Julieta Grajales-Conesa ◽

Liliana Carolina Córdova-Albores

Keyword(s):

Antifungal Activity ◽

Colletotrichum Gloeosporioides ◽

Physicochemical Characterization ◽

Stingless Bee ◽

Melipona Beecheii ◽

Bee Pollen ◽

Scaptotrigona Mexicana ◽

Tetragonisca Angustula

La interacción planta-abeja puede generar productos de la colmena con diferentes características fisicoquímicas, bioactivos y actividad antimicrobiana. Por lo cual, en este trabajo se determinó la composición química de conglomerados o agregados de polen colectados de 12 colmenas establecidas en Chiapas, México, en los municipios Tapachula, Mazatán y Cacahoatán, dentro de tres meliponarios comerciales asociados a las especies: Melipona beecheii, Scaptotrigona mexicana y Tetragonisca angustula. Asimismo, se evaluó el efecto de los agregados de polen en Colletotrichum gloeosporioides. Se encontró una composición química muy diversa independientemente de la especie de abeja. El polen obtenido de colmenas con M. beecheii tuvieron la mayor cantidad de fenoles, flavonoides y acidez libre. Estas propiedades, en adición de la capacidad antioxidante (trolox), glucosa y pH, estuvieron asociados a la inhibición del crecimiento in vitro de C. gloeosporioides. La velocidad de crecimiento radial del hongo durante nueve días fue de 0.013 a 0.009 mm h-1 con extractos de polen, 44 % menor que el efecto del clorotalonil. La actividad antifúngica de los extractos de polen fue de 65 y 37 % para M. beecheii, 57 y 16 % para T. angustula y 60 y 30 % para S. mexicana, respecto al tratamiento testigo y a la dosis más alta de clorotalonil, respectivamente.

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