scholarly journals Antifungal Properties of Pure Silver Films with Nanoparticles Induced by Pulsed-Laser Dewetting Process

2020 ◽  
Vol 10 (7) ◽  
pp. 2260
Author(s):  
Ying-Hong Lin ◽  
Jyun-Jhih Wang ◽  
Yung-Ting Wang ◽  
Hsuan-Kai Lin ◽  
Yi-Jia Lin
Keyword(s):  
Antifungal Activity ◽  
Contact Time ◽  
Colletotrichum Gloeosporioides ◽  
Silver Particles ◽  
Mango Fruit ◽  
Dewetting Process ◽  
Silver Films ◽  
Antifungal Effect ◽  
Ag Particles ◽  
Pathogenicity Tests

Silver particles were prepared by dewetting Ag films coated on glass using a fiber laser. The size of the particles was controlled in the range of 92 nm–1.2 μm by adjusting the thickness of the Ag film. The structural properties and surface roughness of the particles were evaluated by means of scanning electron microscopy. In addition, the antifungal activity of the Ag particles was examined using spore suspensions of Colletotrichum gloeosporioides. It is shown that particles with a size of 1.2 μm achieved 100% inhibition of conidia growth of C. gloeosporioides after a contact time of just 5 min. Furthermore, the smaller particles also achieved good antifungal activity given a longer contact time. Similar results were observed for spore germination and pathogenicity tests performed on mango fruit and leaves. Overall, the results confirm that Ag particles have an excellent antifungal effect on C. gloeosporioides.

scholarly journals Antibacterial Properties of Pure Silver Films with Nanoparticles Induced by Pulsed-Laser Dewetting Process

2020 ◽  
Author(s):  
Y. H. Lin ◽  
J. J. Wang ◽  
Y. T. Wang ◽  
H. K. Lin ◽  
Y. J Lin
Keyword(s):  
Contact Time ◽  
Colletotrichum Gloeosporioides ◽  
Antibacterial Properties ◽  
Mango Fruit ◽  
Dewetting Process ◽  
Silver Films ◽  
Antifungal Effect ◽  
Good Antibacterial Activity ◽  
Ag Particles ◽  
Pathogenicity Tests

Silver particles are prepared by dewetting Ag films coated on glass using a fiber laser. The size of the particles is controlled in the range of 92 nm ~ 1.2 μm by adjusting the thickness of the Ag film. The structural properties and surface roughness of the particles are evaluated by means of scanning electron microscopy. In addition, the antifungal activity of the Ag particles is examined using spore suspensions of Colletotrichum gloeosporioides. It is shown that the particles with a size of 1.2 μm achieve 100% inhibition of the conidia growth of the Colletotrichum gloeosporioides after a contact time of just 5 min. Furthermore, the smaller particles also achieve a good antibacterial activity given a longer contact time. Similar results are observed in spore germination and pathogenicity tests performed on mango fruit and leaves. Overall, the results confirm that the Ag particles have an excellent antifungal effect on Colletotrichum gloeosporioides.

scholarly journals Effet antifongique de deux extraits de plantes sur les agents pathogènes identifiés sur des fruits de l’anacardier (Anacardium occidentale L.) au Nord Cameroun

2021 ◽  
Vol 15 (3) ◽  
pp. 1121-1139
Author(s):  
Aurélie Irène Claire Ngobisa Nyaka ◽  
Sine Nsangou Mfiya Zahrah Fadimatou ◽  
Camille Ulrich Dzoyem Dzokouo ◽  
Sali Bourou ◽  
Aoudou Yaouba
Keyword(s):  
Aspergillus Niger ◽  
Fusarium Solani ◽  
Colletotrichum Gloeosporioides ◽  
Anacardium Occidentale ◽  
Neem Oil ◽  
Antifungal Effect ◽  
Pathogenicity Tests ◽  
Cashew Tree

L’anacardier (Anacardium occidentale L.), importante culture de rente, est confrontée à la menace de nombreux pathogènes qui entravent sa productivité au Cameroun. Dès lors, cette étude se propose de déterminer le potentiel d’une lutte biologique à base d’extraits végétaux sur les champignons pathogènes identifiés sur des fruits prélevés dans le bassin de la Benoué. Pour atteindre ces objectifs, l’identification des agents pathogènes des fruits, des tests de pathogénicité et l’évaluation de l’effet antifongique in vitro et in vivo des extraits de piments et de neem ont été effectués. Les résultats ont montré que ces fruits abritent une diversité d’espèces fongiques dont les principales sont Colletotrichum gloeosporioides, Oïdium anacardii, Fusarium solani, Aspergillus niger et Phytophthora sp. Les tests de pathogénicité ont produit les symptômes des maladies observées sur le terrain. L’huile de neem et les extrait de piment ont inhibé totalement la croissance de C. gloeosporioides, O. anacardii et A. niger aux doses respectives de 7,5% v/v et 75 mg/ml. Cependant, l'huile de neem s'est avérée plus efficace. Ces résultats représentent des sources d'information précieuses pour l’implémentation d'une stratégie de lutte intégrée contre les agents pathogènes de l’anacardier à l’origine de la baisse de sa productivité, affectant ainsi son potentiel économique. English Title: Antifungal effect of two plant extracts on pathogens identified on fruits of Cashew tree (Anacardium occidentale L.) in North Cameroon The Cashew tree (Anacardium occidentale L.), an important cash crop, is challenged by the proliferation of pathogens that affect its productivity in Cameroon. Therefore, this study aims to determine the potential of a biological control based on plant extracts on pathogenic fungi identified on fruits collected in the Benoué basin. To achieve these objectives, identification of fruit pathogens, pathogenicity tests and evaluation of the in vitro and in vivo antifungal effect of chilli and neem extracts were carried out. The results showed that these fruits harbour a diversity of fungal species, of which the main ones are Colletotrichum gloeosporioides, Oïdium anacardii, Fusarium solani, Aspergillus niger and Phytophthora sp. The pathogenicity tests have generated the symptoms of the diseases observed in the field. Neem oil and chilli extracts completely inhibited the growth of C. gloeosporioides, O. anacardii and A. niger at doses of 7.5% v/v and 75 mg/ml respectively. However, neem oil proved to be more effective. These results represent valuable sources of information for the implementation of an integrated pest management strategy against cashew pathogens that impede the productivity of the tree, thereby affecting its economic potential.

scholarly journals Antifungal Activity of Propyl Disulfide from Neem (Azadirachta indica) in Vapor and Agar Diffusion Assays against Anthracnose Pathogens (Colletotrichum gloeosporioides and Colletotrichum acutatum) in Mango Fruit

2021 ◽  
Vol 9 (4) ◽  
pp. 839
Author(s):  
Muhammad Rafiullah Khan ◽  
Vanee Chonhenchob ◽  
Chongxing Huang ◽  
Panitee Suwanamornlert
Keyword(s):  
Antifungal Activity ◽  
Mycelial Growth ◽  
Colletotrichum Gloeosporioides ◽  
Colletotrichum Acutatum ◽  
Model Parameters ◽  
Pathogenicity Test ◽  
Agar Diffusion ◽  
Wide Range ◽  
Significant Difference ◽  
Diffusion Assay

Microorganisms causing anthracnose diseases have a medium to a high level of resistance to the existing fungicides. This study aimed to investigate neem plant extract (propyl disulfide, PD) as an alternative to the current fungicides against mango’s anthracnose. Microorganisms were isolated from decayed mango and identified as Colletotrichum gloeosporioides and Colletotrichum acutatum. Next, a pathogenicity test was conducted and after fulfilling Koch’s postulates, fungi were reisolated from these symptomatic fruits and we thus obtained pure cultures. Then, different concentrations of PD were used against these fungi in vapor and agar diffusion assays. Ethanol and distilled water were served as control treatments. PD significantly (p ≤ 0.05) inhibited more of the mycelial growth of these fungi than both controls. The antifungal activity of PD increased with increasing concentrations. The vapor diffusion assay was more effective in inhibiting the mycelial growth of these fungi than the agar diffusion assay. A good fit (R2, 0.950) of the experimental data in the Gompertz growth model and a significant difference in the model parameters, i.e., lag phase (λ), stationary phase (A) and mycelial growth rate, further showed the antifungal efficacy of PD. Therefore, PD could be the best antimicrobial compound against a wide range of microorganisms.

scholarly journals Biodirected Synthesis of Silver Nanoparticles Using Aqueous Honey Solutions and Evaluation of Their Antifungal Activity against Pathogenic Candida Spp.

2021 ◽  
Vol 22 (14) ◽  
pp. 7715
Author(s):  
Grzegorz Czernel ◽  
Dominika Bloch ◽  
Arkadiusz Matwijczuk ◽  
Jolanta Cieśla ◽  
Monika Kędzierska-Matysek ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Antifungal Activity ◽  
Fungal Pathogens ◽  
Fungal Growth ◽  
Disc Diffusion ◽  
Candida Spp ◽  
Antifungal Effect ◽  
Synthesis Of Nanoparticles ◽  
Honey Solution ◽  
Diffusion Assay

Silver nanoparticles (AgNPs) were synthesized using aqueous honey solutions with a concentration of 2%, 10%, and 20%—AgNPs-H2, AgNPs-H10, and AgNPs-H20. The reaction was conducted at 35 °C and 70 °C. Additionally, nanoparticles obtained with the citrate method (AgNPs-C), while amphotericin B (AmB) and fluconazole were used as controls. The presence and physicochemical properties of AgNPs was affirmed by analyzing the sample with ultraviolet–visible (UV–Vis) and fluorescence spectroscopy, scanning electron microscopy (SEM), and dynamic light scattering (DLS). The 20% honey solution caused an inhibition of the synthesis of nanoparticles at 35 °C. The antifungal activity of the AgNPs was evaluated using opportunistic human fungal pathogens Candida albicans and Candida parapsilosis. The antifungal effect was determined by the minimum inhibitory concentration (MIC) and disc diffusion assay. The highest activity in the MIC tests was observed in the AgNPs-H2 variant. AgNPs-H10 and AgNPs-H20 showed no activity or even stimulated fungal growth. The results of the Kirby–Bauer disc diffusion susceptibility test for C. parapsilosis strains indicated stronger antifungal activity of AgNPs-H than fluconazole. The study demonstrated that the antifungal activity of AgNPs is closely related to the concentration of honey used for the synthesis thereof.

scholarly journals A preliminary study on antifungal effect of TiO2-based paints in natural indoor light

2010 ◽  
Vol 12 (4) ◽  
pp. 53-57 ◽  
Author(s):  
Agata Markowska-Szczupak ◽  
Krzysztof Ulfig ◽  
Barbara Grzmil ◽  
Antoni Morawski
Keyword(s):  
Antifungal Activity ◽  
Aspergillus Niger ◽  
Trichoderma Viride ◽  
Photocatalytic Reaction ◽  
X Ray Diffraction ◽  
Antifungal Effect ◽  
X Ray ◽  
Preliminary Study ◽  
Crystalline Forms ◽  
Growth Of Fungi

A preliminary study on antifungal effect of TiO2-based paints in natural indoor light The antifungal activity of four commercial photocatalytic paints (KEIM Ecosil ME, Titanium FA, Photo Silicate and Silicate D) in natural indoor light was investigated. The paints contained TiO2 in rutile and anatase crystalline forms as evidenced by means of the X-ray diffraction analysis. In most cases the paints inhibited growth of fungi viz. Trichoderma viride, Aspergillus niger, Coonemeria crustacea, Eurotium herbariorum, and Dactylomyces sp. The KEIM Ecosil ME paint displayed the highest antifungal effect in the light, which could be explained with the highest anatase content. The paint antifungal activity and the fungal sensitivity to the TiO2-mediated photocatalytic reaction both decreased in the following orders: KEIM Ecosil ME > Titanium FA > Photo Silicate > Silicate D and T. viride > Dactylomyces sp. > A. niger > E. herbariorum.

scholarly journals Antifungal activity of components used for decontamination of dental prostheses on the growth of Candida albicans

2014 ◽  
Vol 43 (2) ◽  
pp. 137-142 ◽  
Author(s):  
Cíntia Lima Gouveia ◽  
Isabelle Cristine Melo Freire ◽  
Maria Luísa de Alencar e Silva Leite ◽  
Rebeca Dantas Alves Figueiredo ◽  
Leopoldina de Fátima Dantas de Almeida ◽  
...  
Keyword(s):  
Candida Albicans ◽  
Antifungal Activity ◽  
Sodium Bicarbonate ◽  
Fungal Growth ◽  
Antifungal Effect ◽  
Dental Prostheses ◽  
Diffusion Technique ◽  
Zones Of Inhibition ◽  
And Control

Introduction: The effectiveness of antimicrobial solutions employed in dental prosthesis decontamination is still uncertain. Aim: To evaluate the antifungal activity of cleaners used in the decontamination of dental prostheses on the growth of Candida albicans. Material and method: The evaluated products were: Corega Tabs(r) (S1), Sodium Hypochlorite 1% (S2), Sodium Bicarbonate 1% (S3), Hydrogen Peroxide 1% (S4), Chlorhexidine Digluconate 0.12% - Periogard (r) (S5), Mouthrinse based on essential oils - Listerine(r) (S6), essential oil from Rosmarinus officinalis (rosemary) at concentrations of 1% (S7) and 2% (S8). The antifungal activity of the products was evaluated by agar diffusion technique and the determination of microbial death curve of samples of C. albicans (ATCC 90028) in concentration 1.5 × 106 CFU/mL. The tests were performed in triplicate and statistical analysis was made by ANOVA Two-Way and Tukey tests, with the confidence level of 95%. Result: The average of the zones of inhibition growth, in millimeters, obtained for the products were: 0.0 (S1), 44.7 (S2), 0.0 (S3), 21.6 (S4), 10.0 (S5), 6.1 (S6), 0.0 (S7) and 2.4 (S8). Considering the determination of microbial death curve, all products showed a statistical difference (p<0.01) from control (0.85% sodium chloride) and S3 groups. Fungal growth less than 2×104 CFU/mL and an accentuation of the microbial death curve were observed after 30 minutes, with exception for S3 and control groups. Conclusion: The studied compounds, with the exception of Sodium Bicarbonate, have antifungal effect against C. albicans, which contribute for dental prostheses hygiene.

scholarly journals Physicochemical characterization, antioxidant and antifungal activity of three stingless bee pollen aggregate (Apidae: Meliponini) from Soconusco, Chiapas

2020 ◽  
Vol 39 (1) ◽  
Author(s):  
Víctor Albores-Flores ◽  
Erick Saavedra-Camacho ◽  
José Alfonso López-García ◽  
Julieta Grajales-Conesa ◽  
Liliana Carolina Córdova-Albores
Keyword(s):  
Antifungal Activity ◽  
Colletotrichum Gloeosporioides ◽  
Physicochemical Characterization ◽  
Stingless Bee ◽  
Melipona Beecheii ◽  
Bee Pollen ◽  
Scaptotrigona Mexicana ◽  
Tetragonisca Angustula

<p>La interacción planta-abeja puede generar productos de la colmena con diferentes características fisicoquímicas, bioactivos y actividad antimicrobiana. Por lo cual, en este trabajo se determinó la composición química de conglomerados o agregados de polen colectados de 12 colmenas establecidas en Chiapas, México, en los municipios Tapachula, Mazatán y Cacahoatán, dentro de tres meliponarios comerciales asociados a las especies: <em>Melipona beecheii</em>, <em>Scaptotrigona mexicana</em> y <em>Tetragonisca angustula</em>. Asimismo, se evaluó el efecto de los agregados de polen en <em>Colletotrichum gloeosporioides</em>. Se encontró una composición química muy diversa independientemente de la especie de abeja. El polen obtenido de colmenas con <em>M. beecheii</em> tuvieron la mayor cantidad de fenoles, flavonoides y acidez libre. Estas propiedades, en adición de la capacidad antioxidante (trolox), glucosa y pH, estuvieron asociados a la inhibición del crecimiento <em>in vitro</em> de<em> C. gloeosporioides</em>. La velocidad de crecimiento radial del hongo durante nueve días fue de 0.013 a 0.009 mm h-1 con extractos de polen, 44 % menor que el efecto del clorotalonil. La actividad antifúngica de los extractos de polen fue de 65 y 37 % para <em>M. beecheii</em>, 57 y 16 % para <em>T. angustula</em> y 60 y 30 % para S. mexicana, respecto al tratamiento testigo y a la dosis más alta de clorotalonil, respectivamente.</p>

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