Physicochemical characterization, antioxidant and antifungal activity of three stingless bee pollen aggregate (Apidae: Meliponini) from Soconusco, Chiapas

La interacción planta-abeja puede generar productos de la colmena con diferentes características fisicoquímicas, bioactivos y actividad antimicrobiana. Por lo cual, en este trabajo se determinó la composición química de conglomerados o agregados de polen colectados de 12 colmenas establecidas en Chiapas, México, en los municipios Tapachula, Mazatán y Cacahoatán, dentro de tres meliponarios comerciales asociados a las especies: Melipona beecheii, Scaptotrigona mexicana y Tetragonisca angustula. Asimismo, se evaluó el efecto de los agregados de polen en Colletotrichum gloeosporioides. Se encontró una composición química muy diversa independientemente de la especie de abeja. El polen obtenido de colmenas con M. beecheii tuvieron la mayor cantidad de fenoles, flavonoides y acidez libre. Estas propiedades, en adición de la capacidad antioxidante (trolox), glucosa y pH, estuvieron asociados a la inhibición del crecimiento in vitro de C. gloeosporioides. La velocidad de crecimiento radial del hongo durante nueve días fue de 0.013 a 0.009 mm h-1 con extractos de polen, 44 % menor que el efecto del clorotalonil. La actividad antifúngica de los extractos de polen fue de 65 y 37 % para M. beecheii, 57 y 16 % para T. angustula y 60 y 30 % para S. mexicana, respecto al tratamiento testigo y a la dosis más alta de clorotalonil, respectivamente.

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Evaluation of the antifungal activity by plant extracts against Colletotrichum gloeosporioides Penz

Ciência e Agrotecnologia ◽

10.1590/s1413-70542008000200012 ◽

2008 ◽

Vol 32 (2) ◽

pp. 420-428 ◽

Cited By ~ 9

Author(s):

Polyanna Alves Silva ◽

Denilson Ferreira Oliveira ◽

Ney Robson Taironi do Prado ◽

Douglas Antônio de Carvalho ◽

Gilvane Aparecida de Carvalho

Keyword(s):

Antifungal Activity ◽

Freeze Drying ◽

Colletotrichum Gloeosporioides ◽

Plantago Lanceolata ◽

Stevia Rebaudiana ◽

Tween 80 ◽

In Vitro Assays ◽

Bioactive Substances ◽

Stevia Rebaudiana Bertoni

Aiming to develop more efficient and environmental friendly methods than those available to control Colletotrichum gloeosporioides Penz, which causes blister spot in coffee trees, a search for plants able to produce substances active against such pathogen was carried out. Thus, extracts of 48 plant species, collected at Alto Rio Grande region, in Minas Gerais, were prepared and submitted to in vitro assays with that fungus. The best results were obtained with the extracts prepared from Digitalis lanata Ehrh, Origanum manjorona L., Plantago lanceolata Hook. and Stevia rebaudiana (Bertoni) Bertoni, which inhibited C. gloeosporioides spores germination. After dilution of some active extracts with aqueous 1 % Tween 80 solution in a 1:2 or 1:3 ratio (extract:aqueous solution), their antifungal activity vanished. Some of the active extracts were also submitted to freeze drying and none of them presented any alteration in their antifungal activity. Concluding, several plants presented potential to be used in the search for new bioactive substances to control C. gloeosporioides, especially O. manjorona L., which inhibited 96 % of the fungus spores germination.

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Propiedad antifúngica de mieles sobre el desarrollo in vitro de Colletotrichum gloeosporioides

Revista Mexicana de Fitopatología Mexican Journal of Phytopathology ◽

10.18781/r.mex.fit.1805-3 ◽

2018 ◽

Vol 36 (3) ◽

Author(s):

Víctor Albores-Flores ◽

Ivana Janet Marín-Saenz ◽

José Alfonso López-García ◽

Adriana Sánchez-Gutiérrez ◽

Julieta Grajales-Conesa

Keyword(s):

Colletotrichum Gloeosporioides ◽

Scaptotrigona Mexicana

Aunado a la alta toxicidad de los productos químicos para el control fitosanitario de las enfermedades fúngicas a nivel de campo, el microorgansimso causante de enfermedad no es completamente eliminado, por lo que, productos de origen natural tanto vegetal como animal han generado interés para el control de plagas y enfermedades en las plantas. El presente trabajo tuvo como objetivo principal evaluar la actividad antifúngica de mieles de abeja en el crecimiento in vitro de Colletotrichum gloeosporioides. Se evaluaron nueve muestras de mieles, correspondientes a tres especies de abejas: Melipona solani, M. beecheii y Scaptotrigona mexicana. Se encontró que el diámetro de la colonia de C. gloeosporioides fue menor a mayor concentración de miel, con un 40% menos crecimiento de la colonia respecto al tratamiento testigo a los 12 días de incubación. En general la tasa de crecimiento de la colonia del hongo en las mieles correspondientes a los tres tipos de abeja fue en promedio de 40% menos que el testigo. El valor de porcentaje de inhibición observada en las mieles de abeja fue 70% mayor a lo obtenido con el fungicida Clorotalonil.

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ANTIFUNGAL EFFICIENCY OF COPPER OXYCHLORIDE-CONJUGATED SILVER NANOPARTICLES AGAINST COLLETOTRICHUM GLOEOSPORIOIDES WHICH CAUSES ANTHRACNOSE DISEASE

Asian Journal of Pharmaceutical and Clinical Research ◽

10.22159/ajpcr.2019.v12i18.34297 ◽

2019 ◽

pp. 230-233

Author(s):

RAGHAVENDRA SN ◽

RAGHU HS ◽

DIVYASHREE K ◽

RAJESHWARA AN

Keyword(s):

Silver Nanoparticles ◽

Antifungal Activity ◽

Colletotrichum Gloeosporioides ◽

Average Particle Size ◽

Average Particle ◽

Copper Oxychloride ◽

Neem Extract ◽

Anthracnose Disease ◽

Antifungal Efficiency

Objectives: Anthracnose disease is caused by Colletotrichum gloeosporioides, affecting most of the fruit and vegetable plants. The present study is aimed to synthesize silver nanoparticles (AgNPs) using neem extract and conjugate then with fungicide to check the antifungal activity against anthracnose disease. Methods: In the current study, we have synthesized copper oxychloride-conjugated AgNPs (COC-AgNPs) by a biological method using neem extract and have tested their effectiveness against C. gloeosporioides. The COC-AgNPs were characterized by UV–visible spectroscopy, fourier-transform infrared, scanning electron microscopy, and X-ray diffraction analysis, and in vitro antifungal activity was investigated. Results: The shape of COC-AgNPs was found to be spherical with an average particle size of 21–25 nm. The fungicide-conjugated AgNPs exhibited highest growth inhibition of C. gloeosporioides (~187%) as compared to fungicide copper oxychloride. Conclusion: These results indicate that the COC-AgNPs could be effectively used to control anthracnose disease in mango and in other crops. These COC-AgNPs can drastically reduce the amount of fungicide currently used which will reduce the environmental pollution caused by the fungicide.

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Effect of aqueous and alcohol extracts of Phytolacca tetramera (Phytolaccaceae) leaves on Colletotrichum gloeosporioides (Ascomycota) Efecto de los extractos acuoso y alcohólico de la hoja de Phytolacca tetramera (Phytolaccaceae) sobre Colletotrichum...

Boletín de la Sociedad Argentina de Botánica ◽

10.31055/1851.2372.v48.n2.6205 ◽

1970 ◽

Vol 48 (2) ◽

pp. 201-209 ◽

Cited By ~ 2

Author(s):

Marcelo Hernández ◽

Mónica Murace ◽

Jorge Ringuelet ◽

Inés Petri ◽

Daniel Gallo ◽

...

Keyword(s):

Antifungal Activity ◽

Colletotrichum Gloeosporioides ◽

Buenos Aires ◽

Buenos Aires Province ◽

High Concentration ◽

Mycelium Growth ◽

Fungicidal Action ◽

Alcohol Extract ◽

Conidial Production

Summary: Phytolacca tetramera Hauman “ombusillo” is an endemism of southeastern Buenos Aires province (Argentina). This species has fungicidal action against opportunistic pathogens of humans. In order to search natural alternatives for the control of diseases in plants caused by fungi, the objective was to evaluate the effects of aqueous and alcohol extracts of P. tetramera leaves on Colletotrichum gloeosporioides(Penz.) Sacc. This fungus has a wide distributionin different species with agricultural, forestry, and ornamental value. The antifungal activity of aqueous and ethanol leaf extracts was assessed in vitro against fungi. The fungus was subjected to two types of extracts already incorporated into the Potato Dextrose Agar (PDA) medium at 5-50% concentrations. The aqueous extract concentrations within the range 15-30% led to a decrease in the average diameter and speed of mycelium growth, while the range of 15-40% was the most effective in relation to a decrease in conidial production. Also, leaf alcohol extract inhibited the conidial production at concentrations of 5%, and had fungicidal action at concentrations of 15%. From “ombusillo” leaves a foam index of 250 was obtained. This high concentration of saponins would be at least one cause of the antifungal activity.Key words: Antifungal activity in vitro, biological control, “ombusillo”, Phytolaccaceae, saponins.Resumen: Efecto de los extractos acuoso y alcohólico de la hoja de Phytolacca tetramera (Phytolaccaceae) sobre Colletotrichum gloeosporioides (Ascomycota). Phytolacca tetramera Hauman “ombusillo” es un endemismo del sudeste de la provincia de Buenos Aires (Argentina). Esta especie presenta acción fungicida contra patógenos oportunistas de humanos. Con el propósito de buscar alternativas naturales para el control de enfermedades en los vegetales, se planteó como objetivo evaluar el efecto de los extractos foliares acuoso y alcohólico de P. tetramera sobre el desarrollo del hongo Colletotrichum gloeosporioides (Penz.) Sacc., el cual tiene amplia distribución en especies de importancia agrícola, forestal y ornamental. El ensayo se realizó in vitro. El hongo fue cultivado en agar papa glucosado (APG), con aplicación del extracto en concentraciones del 5-50%. Las concentraciones del extracto acuoso del 15-30% produjeron una disminución del diámetro y velocidad media de crecimiento del micelio, mientras que las concentraciones del 15-40% fueron las más efectivas en el control de producción de conidios. El extracto alcohólico inhibió la producción de conidios con el 5% de concentración y con el 15% resultó fungicida. A partir de las hojas de “ombusillo” se obtuvo un índice de espuma de 250. Esta alta concentración de saponinas hace suponer que sería, al menos, una de las causas de la actividad antifúngica.Palabras clave: Actividad antifúngica in vitro, control biológico, “ombusillo”, Phytolaccaceae, saponinas

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In vitro antifungal activity of dimethyl trisulfide against Colletotrichum gloeosporioides from mango

World Journal of Microbiology and Biotechnology ◽

10.1007/s11274-019-2781-z ◽

2019 ◽

Vol 36 (1) ◽

Cited By ~ 2

Author(s):

Lihua Tang ◽

Jianyou Mo ◽

Tangxun Guo ◽

Suiping Huang ◽

Qili Li ◽

...

Keyword(s):

Antifungal Activity ◽

Colletotrichum Gloeosporioides ◽

Dimethyl Trisulfide ◽

In Vitro Antifungal Activity

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Antifungal activity of honey from stingless bee Melipona beecheii against Candida albicans

Journal of Apicultural Research ◽

10.1080/00218839.2019.1665247 ◽

2019 ◽

Vol 59 (1) ◽

pp. 12-18 ◽

Cited By ~ 1

Author(s):

Nidia Esther Hau-Yama ◽

Denis Magaña-Ortiz ◽

A. I. Oliva ◽

Elizabeth Ortiz-Vázquez

Keyword(s):

Candida Albicans ◽

Antifungal Activity ◽

Stingless Bee ◽

Melipona Beecheii

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Chemical composition and in vitroantifungal activity of turmeric oilfrom Curcuma longa L. rhizomeagainst Colletotrichum spp.

Ministry of Science and Technology, Vietnam ◽

10.31276/vjst.63(6).06-10 ◽

2021 ◽

Vol 63 (6) ◽

pp. 6-10

Author(s):

Trung Hieu Pham ◽

◽

Dai Lam Tran ◽

Thi Nam Pham ◽

Dinh Hoang Vu ◽

...

Keyword(s):

Antifungal Activity ◽

Colletotrichum Gloeosporioides ◽

Curcuma Longa ◽

Inhibitory Effect ◽

Volatile Components ◽

In Vitro Growth ◽

Strong Inhibition ◽

Colletotrichum Spp ◽

Poisoned Food Technique

The production of curcumin produces large quantities of the spent turmeric oleoresin as a by-product, which often pollutes the environment. This study was conducted to obtain turmeric oil from this by-product and evaluate its antifungal activity. Turmeric oil afforded by partitioning the turmeric oleoresin from the production of curcumin (Curcuma longa L.) with n-hexane showed a strong inhibition for Colletotrichum species. The volatile components of turmeric oil were determined by GC-MS. Then, 23 volatile compounds were identified from this oil, of which four main compounds were α-zingiberene, α-turmerone, ar-turmerone, and β-sesquiphellandrene. Besides, the authors also isolated Colletotrichum gloeosporioides from lychee fruits, which were harvested in Luc Ngan district, Bac Giang province. By poisoned food technique, the antifungal activity of turmeric oil against C. gloeosporioides, C. orbiculare, C. acutatum, Phytophthora infestans, and Fusarium oxysporum was demonstrated. The results showed that at a concentration of 1 mg/ml, turmeric oil showed the highest inhibitory effect on C. gloeosporioides (67.9%). As for other fungi, in vitro growth inhibition varied in the range of 41-62%.

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