Influence of growth curve phase on electricity performance of microbial fuel cell by Escherichia coli

Generating electricity using microbial fuel cell powered by benthic mud collected from two locations in Akure was carried out. The locations were Riverbed of FUTA and Apatapiti area of Akure. This was achieved by building anode and cathode containers connected together by a salt bridge and an external circuit was made to transfer the electrons from the anode to the cathode. Bacteria and fungi were isolated from the benthic mud for eight days using standard microbiological techniques. Lactobacillus plantarum, Escherichia coli, Bacillus subtilis, Enterobacter aerogenes, Trichoderma sp, Mucor sp and Alterania sp; Lactobacillus plantarum, Escherichia coli, Pseudomonas aeruginosa, Bacillus subtilis, Myrothecium sp and Geotrichum candidum were bacteria and fungi isolated from the benthic mud of Apatapiti area and Riverbed of Futa, Akure respectively. This was used for the generation of electricity using unsterilized mud sample and the control setup was sterilized mud from same source. The set-up was monitored every 24hrous to determine the voltage and current generated. The pH, concentration and temperature were measured. The temperature remains constant throughout the experiment. The set-up were operated at a normal temperature of 27oC and 29oC for Riverbed of FUTA and Apatapiti area of Akure respectively. The peak voltage was between 182.5V and 192.5V and current produced from the main set-up was between 0.3A to 0.53A for Futa river bed while for Apatapiti area of Akure the peak voltage and current were 192.5V and 0.3A respectively. Higher microbial population, current and voltage were observed to be generated in River bed of Futa than Apatapiti area. The difference in the voltage and current and the control set-up shows that anaerobic microorganisms are capable of producing electricity from microbial fuel cell under appropriate conditions.

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Growth Curve Analysis of Rhizobium leguminosarum Using Voltage Produced by Microbial Fuel Cell

International Journal of Life-Sciences Scientific Research ◽

10.21276/ijlssr.2018.4.6.7 ◽

2018 ◽

Vol 4 (6) ◽

pp. 2111-2115

Author(s):

Shrirang R. Maddalwar ◽

Arti S. Shanware

Keyword(s):

Fuel Cell ◽

Microbial Fuel Cell ◽

Growth Curve ◽

Rhizobium Leguminosarum ◽

Curve Analysis ◽

Growth Curve Analysis

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A novel mediatorless microbial fuel cell based on direct biocatalysis of Escherichia coli

Chemical Communications ◽

10.1039/b600876c ◽

2006 ◽

pp. 2257 ◽

Cited By ~ 110

Author(s):

Tian Zhang ◽

Changzheng Cui ◽

Shengli Chen ◽

Xinping Ai ◽

Hanxi Yang ◽

...

Keyword(s):

Escherichia Coli ◽

Fuel Cell ◽

Microbial Fuel Cell

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Pengaruh pH dan Metabolisme Bakteri Escherichia coli dalam Reaktor Microbial Fuel Cell Terhadap Reduksi Kromium Heksavalen

CHEESA Chemical Engineering Research Articles ◽

10.25273/cheesa.v2i2.5481 ◽

2019 ◽

Vol 2 (2) ◽

pp. 83

Author(s):

Prapti Ira Kumalasari ◽

Junety Monde ◽

Zefanya Bernadi Yusuf ◽

Rini Rini

Keyword(s):

Escherichia Coli ◽

Fuel Cell ◽

Microbial Fuel Cell ◽

Power Density ◽

Ph Optimum ◽

Double Chamber

Kalimantan merupakan pulau yang terkenal akan sektor pertambangan salah satunya di daerah delta Mahakam, yang dalam proses eksploitasinya berpotensi menghasilkan limbah logam berat, seperti logam berat Cr6+. Pencemaran logam Cr6+ cukup sulit untuk terurai dilingkungan dan bersifat karsinogenik, karena dengan konsentrasi kecil saja dapat menimbulkan tingkat keracunan yang sangat tinggi pada makhluk hidup, sehingga pengolahan terhadap limbah tersebut sangat penting. Microbial Fuel Cell merupakan suatu metode yang dapat membantu proses pengolahan limbah dengan cara mereduksi Cr6+ menjadi Cr3+ dengan katalisis mikrobiologis. Penelitian ini menggunakan metode reaktor double-chamber yaitu terdapat ruang anoda yang berisi bakteri anaerob dan basic anolyte, sedangkan pada ruang katoda terdapat kalium dikromat dengan konsentrasi 18 mg/L dan variasi pH 3, 4 dan 5 yang dilakukan selama 10 hari. Kondisi pH optimum pada proses reduksi terjadi pada pH 4 dengan besar persen penurunan sekitar 98%. Dan produksi listrik tertinggi pada hari ke-2 pada variasi pH 3 dengan nilai power density sebesar sebesar 11, 06 mW/m2.

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Screening technique on the selection of potent microorganisms for operation in microbial fuel cell for generation of power

Journal of Electrochemical Science and Engineering ◽

10.5599/jese.924 ◽

2021 ◽

Author(s):

Payel Choudhury ◽

Biswanath Bhunia ◽

Tarun Kanti Bandyopadhyaya

Keyword(s):

Escherichia Coli ◽

Fuel Cell ◽

Trace Element ◽

Microbial Fuel Cell ◽

Iron Reduction ◽

Rapid Development ◽

Element Solution ◽

Process Factors ◽

K 12 ◽

Trace Element Solution

This paper focuses on determination of the influence of electrochemically active microorganisms on the transmission of electrons from the respiratory enzymes to the electrode and assembling of exoelectrogens to the simulated wastewater medium. In this study, the total of eight microorganisms were experimentally tested to exhibit growth and high iron-reducing ability in the absence of mediators. A major connection was observed between the growth and iron-reduction ability of the microorganism. The growth and iron-reduction ability were monitored experimentally over time. Based on output data, the screening was done among eight different microorganisms, where Escherichia coli -K-12 was chosen as the most potent microorganism for its wide application in a microbial fuel cell (MFC). In the present study, various biochemical process factors were optimized statistically using Taguchi methodology for the rapid development of growth and iron-reducing assay conditions. The design of various experimental trials was carried out using five process factors at three levels with orthogonal arrays (OA) layout of L18. Five process factors, including quantity of lactose, volume of trace element solution, inoculum percentage, pH, and temperature, were taken into consideration as imperative process factors and optimized for evaluation of growth of bacteria and iron reduction ability. The larger-is-best signal to noise (S/N) ratio, together with analysis of variance ANOVA, were used during optimization. Anticipated results demonstrated that the enhanced bacterial growth of 124.50 % and iron reduction ability of 112.6 % can be achieved with 8 g/L of lactose, 2 ml of trace element solution, 4 % (v/v) of inoculum, pH 7, and temperature of 35 oC. Furthermore, the growth and iron reduction time profiles of Escherichia coli-K12 were performed to determine its feasibility in MFC. Open circuit voltage of 0.555 V was obtained over batch study on a single chamber microbial fuel cell (SCMFC).

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