Utilization of Pistia stratiotes (aquatic weed) for fermentative biohydrogen: Electron-equivalent balance, stoichiometry, and cost estimation

Objective: To investigate the bioactive components of an invasive aquatic weed, Pistia stratiotes L. and Eichhornia crassipes (Mart.) Solms vegetative parts by using Gas Chromatography-Mass Spectrometer (GC-MS).Methods: The chemical compositions of the ethanol extract of whole plant Pistia stratiotes L. and Eichhornia crassipes (Mart.) Solms was investigated using Agilent Technologies GC-MS (GC-7890A, MS 5975C).Results: The results of GC-MS analysis of the ethanolic extract revealed the existence of 28 phytochemical compounds in Pistia stratiotes L. n-Hexadecanoic acid,-11-Hexadecenoic acid, ethyl ester, Hexadecanoic acid, ethyl ester, Octadecanoic acid, ethyl ester, 2-Cyclopenten-1-one, 5-hydroxy-2,3-dimethyl, L-Glutamine, 2-Pentadecanone, 6,10,14-trimethyl, Linolelaidic acid, methyl ester, 9,12,15-Octadecatrienoic acid, methyl ester,(Z,Z,Z), Nonadecane, 12,15-Octadecadiynoic acid, methyl ester, Hexadecanoic acid, 2-hydroxy-1-(hydroxymethyl)ethyl ester, Diisooctyl phthalate, Docosanoic acid, ethyl ester, Stigmasterol, Bis(2-ethylhexyl) phthalate, 1-Monolinoleoylglycerol trimethylsilyl ether, Ethyl iso-allocholate are the major compound.The ethanolic extract of Eichhornia crassipes (Mart.) Solms contains 43 phytochemical compounds of high and low molecular weight n-Hexadecanoic acid, E-11-Hexadecenoic acid, ethyl ester, Palmitic acid, Phytol, 9,12,15-Octadecatrienal, 9,12-Octadecadienoic acid, ethyl ester, Linolenic acid, ethyl ester, Stearic acid, ethyl ester, Hexadecanoic acid, 2-hydroxy-1-(hydroxymethyl)ethyl ester, α-Glyceryl linolenate, 1-Monolinoleoylglycerol trimethylsilyl ether, Linoleic acid, 2,3-bis-(O-TMS)-propyl ester, Stigmasterol, Linolelaidic acid, methyl ester, 9,12,15-Octadecatrienoic acid, ethyl ester, (Z,Z,Z), Ethyl iso-allocholate, Cholesta-22,24-dien-5-ol, 4,4-dimethyl are the major compounds.These results indicates Pistia stratiotes L. and Eichhornia crassipes (Mart.) Solms possess potent antioxidant, anti-inflammatory, anticancer, antitumour, antiarthritic, cancer preventive, antibacterial effects so can be recommended as a plant of phytopharmaceutical importance.Conclusion: The ethanol extract of Pistia stratiotes L. and Eichhornia crassipes (Mart.) Solms proves as a potential source of bioactive compounds of pharmacological importance.

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Studies on the immature stages of the planthopper Lepidelphax pistiae (Hemiptera: Delphacidae), a potential biocontrol agent for the aquatic weed Pistia stratiotes (Araceae) from Argentina

Austral Entomology ◽

10.1111/aen.12248 ◽

2016 ◽

Vol 56 (4) ◽

pp. 384-391 ◽

Cited By ~ 2

Author(s):

Ana M Marino de Remes Lenicov ◽

Barbara Defea ◽

José Rusconi ◽

Guillermo Cabrera Walsh

Keyword(s):

Biocontrol Agent ◽

Pistia Stratiotes ◽

Aquatic Weed ◽

Immature Stages ◽

Potential Biocontrol Agent

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Biohydrogen fermentation from Pistia stratiotes (aquatic weed) using mixed and pure bacterial cultures

International Journal of Hydrogen Energy ◽

10.1016/j.ijhydene.2019.05.152 ◽

2019 ◽

Vol 44 (33) ◽

pp. 17720-17731 ◽

Cited By ~ 5

Author(s):

Nonsikelelo Precios Mthethwa ◽

Mahmoud Nasr ◽

Sammy Lewis Kiambi ◽

Faizal Bux ◽

Sheena Kumari

Keyword(s):

Pistia Stratiotes ◽

Aquatic Weed ◽

Bacterial Cultures ◽

Pure Bacterial Cultures

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EFEITO DA CHUVA SOBRE A AÇÃO DO HERBICIDA IMAZAPYR NO CONTROLE DE PLANTAS DANINHAS AQUÁTICAS.

Irriga ◽

10.15809/irriga.2010v15n2p151 ◽

2010 ◽

Vol 15 (2) ◽

pp. 151-158 ◽

Cited By ~ 2

Author(s):

Caio Ferraz de Campos ◽

Guilherme Sasso Ferreira de Souza ◽

Maria Renata Rocha Pereira ◽

Dagoberto Martins

Keyword(s):

Chemical Control ◽

Water Hyacinth ◽

Eichhornia Crassipes ◽

Pistia Stratiotes ◽

Aquatic Weed ◽

Herbicide Application ◽

Time Intervals ◽

Randomized Design ◽

Completely Randomized Design ◽

And Control

O objetivo deste estudo foi avaliar a influência de diferentes períodos de chuva ocorridos após a aplicação do herbicida imazapyr, em sua eficiência de controle sobre plantas de Pistia stratiotes e Eichhornia crassipes. Foram estudadas chuvas de 10 mm, aplicadas durante 5', em diferentes períodos de tempo (0h, 2h, 4h, 6h, 8h, 12h, 24h e não simulação de chuva) após a aplicação de imazapyr, na formulação Arsenal NA, a 250 g i.a. ha-1(1,0 L p.c. ha-1). O delineamento experimental utilizado foi o inteiramente casualizado, com quatro repetições. As plantas foram avaliadas visualmente aos 7, 14, 21, 28 e 35 dias após a aplicação (DAA), sendo os resultados obtidos submetidos à análise de variância pelo teste F e as médias dos tratamentos comparadas pelo teste de Tukey (p<0,05). A ocorrência chuvas em até 6 horas após a aplicação do herbicida imazapyr, em plantas de P. stratiotes, afetou negativamente sua eficiência de controle e, chuvas após intervalos acima de 8 horas não influenciaram no controle. A ocorrência de chuva em todos os intervalos de tempo, não interferiu no controle de plantas de E. crassipes. UNITERMOS: aguapé, alface-d'água, controle químico, fitointoxicação CAMPOS, C.F.; SOUZA, G.S.F.; PEREIRA, M.R.R.; MARTINS, D. EFFECT OF RAINFALL ON IMAZAPYR ACTION IN AQUATIC WEED CONTROL 2 ABSTRACT The aim of this study was to evaluate the influence of different periods of rainfall that occurred after the application of the imazapyr herbicide on the control efficiency of Pistia stratiotes and Eichhornia crassipes. We studied 10 mm rainfall, applied for 5' at different time periods (0h, 2h, 4h, 6h, 8h, 12h, 24h, not simulated rainfall) after the application of imazapyr formulation Arsenal NA, 250 g ai ha-1 (1.0 L cp ha -1). The experiment had completely randomized design with four replications. Plants were evaluated at 7, 14, 21, 28 and 35 days after application (DAA), and the results submitted to analysis of variance by F test and treatment means compared by Tukey's test (p <0. 05). The occurrence of rainfall up to 6 hours after herbicide application, plants of P. stratiotes, negatively affected its efficiency and control; rainfall intervals over 8 hours had no effect in control. The occurrence of rainfall at all time intervals did not affect the control of E. crassipes plants. KEYWORDS: water hyacinth, waterlettuce, chemical control, phytotoxicity

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Performance of Pistia stratiotes, Salvinia molesta, and Eichhornia crassipes Aquatic Plants in the Tertiary Treatment of Domestic Wastewater with Varying Retention Times

Applied Sciences ◽

10.3390/app10249105 ◽

2020 ◽

Vol 10 (24) ◽

pp. 9105

Author(s):

Hauwa Mohammed Mustafa ◽

Gasim Hayder

Keyword(s):

Eichhornia Crassipes ◽

Domestic Wastewater ◽

Physicochemical Analysis ◽

Pistia Stratiotes ◽

Aquatic Weed ◽

Ammoniacal Nitrogen ◽

Tertiary Treatment ◽

Salvinia Molesta ◽

Hydroponic Systems ◽

Wastewater Samples

Organic matter, nutrients, and chemicals are some of the waste materials found in most domestic wastewaters. The purpose of this research was to evaluate the potentials of Pistia stratiotes, Salvinia molesta, and Eichhornia crassipes aquatic weed plants in the tertiary treatment of secondary domestic wastewater using hydroponic systems. Physicochemical analysis that considered turbidity, phosphate, ammoniacal nitrogen, and nitrate was conducted on the wastewater samples at different retention times of 6, 12, and 24 h for 14 days. The best performance was observed for a 24 h retention treatment system, with up to 91% (P. stratiotes), 94% (S. molesta), and 89.3% (E. crassipes) reduction for turbidity; 81.2% (P. stratiotes), 82.7% (S. molesta), and 88.5% (E. crassipes) for phosphate reduction; 88.66% (P. stratiotes), 90.47% (S. molesta), and 89.11% (E. crassipes) for ammoniacal nitrogen reduction; 83.6% (P. stratiotes), 92.1% (S. molesta) and 93% (E. crassipes) for nitrate reduction. Hence, the application of hydroponic systems in cultivation of the macrophytes proved to be effective in the phytoremediation process by removing the residual nutrients present in the treated secondary domestic wastewater. Additionally, this method can be used for the cultivation of plant-based biomass and other non-edible useful crops using the free nutrients content of the wastewater before its discharge into the natural environment.

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Chemical constituents from the aquatic weed Pistia stratiotes

Chemistry of Natural Compounds ◽

10.1007/s10600-008-9025-z ◽

2008 ◽

Vol 44 (2) ◽

pp. 236-238 ◽

Cited By ~ 5

Author(s):

Han-Wei Liu ◽

Li-Ying He ◽

Jing-Ming Gao ◽

Yun-Bao Ma ◽

Xue-Mei Zhang ◽

...

Keyword(s):

Chemical Constituents ◽

Pistia Stratiotes ◽

Aquatic Weed

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Enhancement of biohydrogen production from the aquatic weed Pistia stratiotes through a dark fermentation process

10.51415/10321/3217 ◽

2019 ◽

Author(s):

◽

Nonsikelelo Precios Mthethwa

Keyword(s):

Hydrogen Production ◽

Fermentation Process ◽

Dark Fermentation ◽

Biohydrogen Production ◽

Pistia Stratiotes ◽

Aquatic Weed ◽

Operational Conditions ◽

Bacillus Spp ◽

Pre Treatment ◽

Clostridium Spp

Aquatic weeds are well known for their fast growth rate and high carbohydrate content that can be easily hydrolysed into fermentable sugars. This study was aimed at the utilization of an indigenous aquatic weed, Pistia stratiotes for biohydrogen production through the dark fermentation process. Characterization of the biomass, effect of pre–treatment methods on biomass hydrolysis, effect of reactor operational conditions and type of inoculum on enhancing hydrogen production potential of P. stratiotes was assessed. Physical and chemical pre–treatments were employed on P. stratiotes biomass to increase digestibility and to achieve high conversion rates of fermentable sugars. The highest sugar yield of 139± 0.8 mg/g was obtained when the oven dried biomass was subjected to H2SO4 (2.5%) pre– treatment followed by autoclaving at 121°C for 30 min. Biohydrogen production under different operational conditions was thereafter optimized using One–factor–at–a–time (OFAT) batch experiments in 120 mL serum bottles. A maximum hydrogen yield (HY) of 2.46 ± 0.14 mol-H2/mol-glucose (3.51 ± 0.20 mg-H2/g-dry weight) and 2.75 ± 0.07 mL h-1 hydrogen production rate was observed under optimized conditions (pH 5.5, Temp 35°C, S/X: 1.0 g-COD/g-VSS and HRT 8 h). The organic mass balance (92 – 96%) and electron– equivalent balance (92 – 98%) further indicated the reliability of the obtained fermentation data. Assessment of microbial activity was achieved using molecular techniques such as quantitative polymerase chain reaction (qPCR) targeting both 16s rRNA (of Clostridium spp., Bacillus spp., and Enterobacter spp.) and the functional hydrogenase gene (hydA). The highest gene activity of hydrogenase was noted at pH of 5.5 with 2.53×104 copies/ng-DNA compared to low pH: 4.5 (6.95 × 103 copies/ng-DNA) and high pH: 8.5 (7.77×103 copies/ng- DNA). A similar trend was also observed for the species containing a highly active hydrogenase (i.e. Clostridium spp., Bacillus spp., and Enterobacter spp.). During the optimum reactor conditions, three hydrogen producing bacterial strains Bacillus cereus and Enterobacter cloacae were successfully isolated. These isolates were used as inoculums for the pure culture studies and achieved HYs of 2.2, 1.10 and 1.97 mol-H2/mol-glucose respectively under optimized fermentation conditions. However, the thermally treated mixed culture displayed a marginally higher HY (2.46 mol-H2/mol-glucose) compared to the pure culture used alone. Furthermore, the cost estimation indicated a potential and economically feasible for biotransformation of P. stratiotes to hydrogen energy. In conclusion, the results from this study has revealed the potential of employing P. stratiotes biomass for biohydrogen production. The results also indicated the importance of employing suitable pre–treatment methods, operating conditions as well as inoculum types for enhanced hydrogen production using P. stratiotes.

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