scholarly journals Emergence of chromosome borne colistin resistance gene, mcr-1 in clinical isolates of Pseudomonas aeruginosa

2020 ◽  
Vol 101 ◽  
pp. 22
Author(s):  
A. Pathak ◽  
S. Singh ◽  
A. Kumar ◽  
K.N. Prasad
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Resistance Gene ◽  
Clinical Isolates ◽  
Colistin Resistance

scholarly journals Aminoglycoside Resistance Gene ant(4′)-IIb of Pseudomonas aeruginosa BM4492, a Clinical Isolate from Bulgaria

2003 ◽  
Vol 47 (5) ◽  
pp. 1584-1588 ◽  
Author(s):  
Stefana Sabtcheva ◽  
Marc Galimand ◽  
Guy Gerbaud ◽  
Patrice Courvalin ◽  
Thierry Lambert
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Resistance Gene ◽  
Clinical Isolate ◽  
Chromosomal Location ◽  
Clinical Isolates ◽  
Aminoglycoside Resistance ◽  
Coding Sequence ◽  
Clinical Strains ◽  
Gram Positive Bacteria ◽  
Calculated Mass

ABSTRACT The ant(4′)-IIb gene of Pseudomonas aeruginosa BM4492, which encodes an aminoglycoside 4′-O-adenylyltransferase, was identified as a coding sequence of 756 bp corresponding to a protein with a calculated mass of 27,219 Da. Analysis of the deduced sequence indicated that the protein was related to aminoglycoside 4′-O-adenylyltransferases IIa and Ia found in P. aeruginosa and gram-positive bacteria, respectively. The enzyme conferred resistance to amikacin and tobramycin but not to dibekacin, gentamicin, or netilmicin. The ant(4′)-IIb gene had a chromosomal location in five of six clinical isolates of P. aeruginosa tested and was plasmid borne in the remaining strain. The ant(4′)-IIb gene was detected by PCR in some clinical strains of P. aeruginosa from the same hospital but not in members of other bacterial genera.

scholarly journals Detection of mcr-1 colistin resistance gene in polyclonal Escherichia coli isolates in Barcelona, Spain, 2012 to 2015

2016 ◽  
Vol 21 (13) ◽  
Author(s):  
Núria Prim ◽  
Alba Rivera ◽  
Judith Rodríguez-Navarro ◽  
Montserrat Español ◽  
Miquel Turbau ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Resistance Gene ◽  
Clonal Diversity ◽  
Pfge Pattern ◽  
Clinical Isolates ◽  
Colistin Resistance ◽  
Local Study ◽  
Study Population

Colistin resistance was detected in 53 of 10,011 Escherichia coli (0.5%) by prospective phenotypic testing of consecutive clinical isolates in a single hospital in Barcelona, Spain (2012–15). The mcr-1 gene was retrospectively identified by PCR and sequencing in 15 of 50 available isolates. Each isolate had a unique PFGE pattern except for two. This clonal diversity supports the hypothesis of horizontal dissemination of the mcr-1 gene in the local study population.

scholarly journals Detection of themcr-1Colistin Resistance Gene in Carbapenem-Resistant Enterobacteriaceae from Different Hospitals in China

2016 ◽  
Vol 60 (8) ◽  
pp. 5033-5035 ◽  
Author(s):  
Hua Yu ◽  
Fen Qu ◽  
Bin Shan ◽  
Bin Huang ◽  
Wei Jia ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Global Health ◽  
Resistance Gene ◽  
Clinical Isolates ◽  
Chromosomal Integration ◽  
Colistin Resistance ◽  
Content Type ◽  
E Coli ◽  
Carbapenem Resistant ◽  
Carbapenem Resistant Enterobacteriaceae

ABSTRACTThe spread of the plasmid-mediated colistin resistance gene,mcr-1, into carbapenem-resistantEnterobacteriaceae(CRE) clinical isolates poses a significant threat to global health. Here we report the identification of threemcr-1-harboring carbapenem-resistantEscherichia colistrains, collected from three patients in two provinces in China. Our results show thatmcr-1-harboring CRE strains have started to spread in different hospitals in China. In addition, this report presents the first description of chromosomal integration ofmcr-1into a carbapenem-resistantE. colistrain.

scholarly journals Emergence of Chromosome-Borne Colistin Resistance Gene mcr-1 in Clinical Isolates of Klebsiella pneumoniae from India

2017 ◽  
Vol 62 (2) ◽  
Author(s):  
Sanjay Singh ◽  
Ashutosh Pathak ◽  
Awadhesh Kumar ◽  
Mohibur Rahman ◽  
Avinash Singh ◽  
...  
Keyword(s):  
Klebsiella Pneumoniae ◽  
Resistance Gene ◽  
Clinical Isolates ◽  
Colistin Resistance

scholarly journals Chromosomally Encoded mcr-5 in Colistin Non-susceptible Pseudomonas aeruginosa

2018 ◽  
Author(s):  
Erik Snesrud ◽  
Rosslyn Maybank ◽  
Yoon I. Kwak ◽  
Anthony R. Jones ◽  
Mary K. Hinkle ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Whole Genome Sequencing ◽  
Genome Sequencing ◽  
Genome Analysis ◽  
Clinical Isolates ◽  
Whole Genome ◽  
Broth Microdilution ◽  
Colistin Resistance ◽  
First Report ◽  
Chromosomal Copy

AbstractWhole genome sequencing (WGS) of historical Pseudomonas aeruginosa clinical isolates identified a chromosomal copy of mcr-5 within a Tn3-like transposon in P. aeruginosa MRSN 12280. The isolate was non-susceptible to colistin by broth microdilution and genome analysis revealed no mutations known to confer colistin resistance. To the best of our knowledge this is the first report of mcr in colistin non-susceptible P. aeruginosa.

scholarly journals Chromosomally Encoded mcr-5 in Colistin-Nonsusceptible Pseudomonas aeruginosa

2018 ◽  
Vol 62 (8) ◽  
Author(s):  
Erik Snesrud ◽  
Rosslyn Maybank ◽  
Yoon I. Kwak ◽  
Anthony R. Jones ◽  
Mary K. Hinkle ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Whole Genome Sequencing ◽  
Genome Sequencing ◽  
Genome Analysis ◽  
Clinical Isolates ◽  
Whole Genome ◽  
Broth Microdilution ◽  
Colistin Resistance ◽  
Content Type ◽  
First Report

ABSTRACT Whole-genome sequencing (WGS) of historical Pseudomonas aeruginosa clinical isolates identified a chromosomal copy of mcr-5 within a Tn3-like transposon in P. aeruginosa MRSN 12280. The isolate was nonsusceptible to colistin by broth microdilution, and genome analysis revealed no mutations known to confer colistin resistance. To the best of our knowledge, this is the first report of mcr in colistin-nonsusceptible P. aeruginosa.

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