Cardiolipin and oxidative stress: Identification of new short chain oxidation products of cardiolipin in in vitro analysis and in nephrotoxic drug-induced disturbances in rat kidney tissue

2011 ◽  
Vol 301 (1-3) ◽  
pp. 62-73 ◽  
Author(s):  
Elisabete Maciel ◽  
Pedro Domingues ◽  
Diane Marques ◽  
Cláudia Simões ◽  
Ana Reis ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Rat Kidney ◽  
Kidney Tissue ◽  
Oxidation Products ◽  
Drug Induced ◽  
Chain Oxidation ◽  
Vitro Analysis ◽  
And Oxidative Stress ◽  
In Vitro Analysis

scholarly journals Bilirubin Production and Oxidation in CSF of Patients with Cerebral Vasospasm after Subarachnoid Hemorrhage

2005 ◽  
Vol 25 (8) ◽  
pp. 1070-1077 ◽  
Author(s):  
Gail J Pyne-Geithman ◽  
Chad J Morgan ◽  
Kenneth Wagner ◽  
Elizabeth M Dulaney ◽  
Janice Carrozzella ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Subarachnoid Hemorrhage ◽  
Cerebral Vasospasm ◽  
Cerebral Spinal Fluid ◽  
Spinal Fluid ◽  
Oxidation Products ◽  
Mortality And Morbidity ◽  
Delayed Cerebral Vasospasm ◽  
And Oxidative Stress

Delayed cerebral vasospasm after subarachnoid hemorrhage (SAH) remains a significant cause of mortality and morbidity; however, the etiology is, as yet, unknown, despite intensive research efforts. Research in this laboratory indicates that bilirubin and oxidative stress may be responsible by leading to formation of bilirubin oxidation products (BOXes), so we investigated changes in bilirubin concentration and oxidative stress in vitro, and in cerebral spinal fluid (CSF) from SAH patients. Non-SAH CSF, a source of heme oxygenase I (HO-1), and blood were incubated, and in vitro bilirubin production measured. Cerebrospinal fluid from SAH patients was collected, categorized using stimulation of vascular smooth muscle metabolism in vitro, and information obtained regarding occurrence of vasospasm in the patients. Cerebral spinal fluid was analyzed for hemoglobin, total protein and bilirubin, BOXes, malonyldialdehyde and peroxidized lipids (indicators of an oxidizing environment), and HO-1 concentration. The formation of bilirubin in vitro requires that CSF is present, as well as whole, non-anti-coagulated blood. Bilirubin, BOXes, HO-1, and peroxidized lipid content were significantly higher in CSF from SAH patients with vasospasm, compared with nonvasospasm SAH CSF, and correlated with occurrence of vasospasm. We conclude that vasospasm may be more likely in patients with elevated BOXes. The conditions necessary for the formation of BOXes are indeed present in CSF from SAH patients with vasospasm, but not CSF from SAH patients without vasospasm.

Stratifin as a Novel Diagnostic Biomarker in Serum for Diffuse Alveolar Damage

2021 ◽  
Author(s):  
Noriaki Arakawa ◽  
Atsuhito Ushiki ◽  
Mitsuhiro Abe ◽  
Shinichiro Matsuyama ◽  
Yoshinobu Saito ◽  
...  
Keyword(s):  
Bronchoalveolar Lavage Fluid ◽  
Diffuse Alveolar Damage ◽  
Lavage Fluid ◽  
A549 Cell Line ◽  
Drug Induced ◽  
Extracellular Release ◽  
P53 Activation ◽  
Vitro Analysis ◽  
In Vitro Analysis

Abstract Among the various histopathological patterns of drug-induced interstitial lung disease (DILD), diffuse alveolar damage (DAD) is associated with poor prognosis. However, there is no reliable biomarker for its accurate diagnosis. Here, we show stratifin/14-3-3σ (SFN) as a biomarker candidate found in a proteomic analysis. The study included two independent cohorts and controls (n = 432 samples). SFN was specifically elevated in DILD patients with DAD, and was superior to the known biomarkers, KL-6 and SP-D, in discrimination of DILD patients with DAD from patients with other DILD patterns or other lung diseases, including bacterial pneumonia. SFN was also increased in serum from patients with idiopathic DAD, and in lung tissues and bronchoalveolar lavage fluid of patients with DAD. In vitro analysis using the A549 cell line suggested that extracellular release of SFN occurred via p53 activation. We conclude that serum SFN is a promising biomarker for DAD diagnosis.

scholarly journals The Neuropeptide Pituitary Adenylate Cyclase-Activating Polypeptide (PACAP) is Protective in Inflammation and Oxidative Stress-Induced Damage in the Kidney

2019 ◽  
Vol 20 (19) ◽  
pp. 4944 ◽  
Author(s):  
Horvath ◽  
Opper ◽  
Reglodi
Keyword(s):  
Oxidative Stress ◽  
Adenylate Cyclase ◽  
Ischemia Reperfusion ◽  
Entire Body ◽  
Drug Induced ◽  
Protective Actions ◽  
Organ Systems ◽  
Pituitary Adenylate Cyclase ◽  
And Oxidative Stress

Pituitary adenylate cyclase-activating polypeptide (PACAP) is a pleiotropic neuropeptide with a widespread distribution throughout the entire body including the urinary system. PACAP exerts protective actions in different injury models related to several organ systems. Its protective effect is mainly based on its antiapoptotic, anti-inflammatory and antioxidant effects. The present review aims to summarize the effects of PACAP in pathologies associated with inflammation and oxidative stress-induced damage in the kidney. Both in vitro and in vivo data are available proving its protective actions against oxidative stress, hypoxia, renal ischemia/reperfusion, diabetic nephropathy, myeloma kidney injury, amyloidosis and different types of drug-induced nephropathies. Data showing the nephroprotection by PACAP emphasize the potential of PACAP’s therapeutic use in various renal pathologies.

scholarly journals In Vitro Analysis of CsA-Induced Hepatotoxicity in HepG2 Cell Line: Oxidative Stress and α2 and β1 Integrin Subunits Expression

2013 ◽  
Vol 13 (8) ◽  
Author(s):  
Zohreh Mostafavi-Pour ◽  
Fatemeh Khademi ◽  
Fatemeh Zal ◽  
Ahmad-Reza Sardarian ◽  
Fatemeh Amini
Keyword(s):  
Oxidative Stress ◽  
Cell Line ◽  
Hepg2 Cell ◽  
Β1 Integrin ◽  
Hepg2 Cell Line ◽  
Vitro Analysis ◽  
In Vitro Analysis

The native structure of the eukaryotic ribosome

1983 ◽  
Vol 41 ◽  
pp. 432-433
Author(s):  
R.A. Milligan ◽  
P.N.T. Unwin
Keyword(s):  
Ribosomal Proteins ◽  
Crystal Form ◽  
Native Structure ◽  
X Ray Diffraction ◽  
Eukaryotic Ribosome ◽  
Vitro Analysis ◽  
In Vitro Analysis ◽  
Resolution Structure ◽  
Stable Unit

A detailed understanding of the mechanism of protein synthesis will ultimately depend on knowledge of the native structure of the ribosome. Towards this end we have investigated the low resolution structure of the eukaryotic ribosome embedded in frozen buffer, making use of a system in which the ribosomes crystallize naturally.The ribosomes in the cells of early chicken embryos form crystalline arrays when the embryos are cooled at 4°C. We have developed methods to isolate the stable unit of these arrays, the ribosome tetramer, and have determined conditions for the growth of two-dimensional crystals in vitro, Analysis of the proteins in the crystals by 2-D gel electrophoresis demonstrates the presence of all ribosomal proteins normally found in polysomes. There are in addition, four proteins which may facilitate crystallization. The crystals are built from two oppositely facing P4 layers and the predominant crystal form, accounting for >80% of the crystals, has the tetragonal space group P4212, X-ray diffraction of crystal pellets demonstrates that crystalline order extends to ~ 60Å.

Electron microscopic study of the membrane glycoproteins in the rat kidney after cisplatin treatment

1989 ◽  
Vol 47 ◽  
pp. 912-913
Author(s):  
S.K. Aggarwal
Keyword(s):  
Alkaline Phosphatase ◽  
Rat Kidney ◽  
Light And Electron Microscopy ◽  
Kidney Tissue ◽  
Membrane Glycoproteins ◽  
Electron Microscopic ◽  
Before And After ◽  
Interstrand Cross Links ◽  
Cross Links

The proposed primary mechanism of action of the anticancer drug cisplatin (Cis-DDP) is through its interaction with DNA, mostly through DNA intrastrand cross-links or DNA interstrand cross-links. DNA repair mechanisms can circumvent this arrest thus permitting replication and transcription to proceed. Various membrane transport enzymes have also been demonstrated to be effected by cisplatin. Glycoprotein alkaline phosphatase was looked at in the proximal tubule cells before and after cisplatin both in vivo and in vitro for its inactivation or its removal from the membrane using light and electron microscopy.Outbred male Swiss Webster (Crl: (WI) BR) rats weighing 150-250g were given ip injections of cisplatin (7mg/kg). Animals were killed on day 3 and day 5. Thick slices (20-50.um) of kidney tissue from treated and untreated animals were fixed in 1% buffered glutaraldehyde and 1% formaldehyde (0.05 M cacodylate buffer, pH 7.3) for 30 min at 4°C. Alkaline phosphatase activity and carbohydrates were demonstrated according to methods described earlier.

1163: Radial Dilation of Ureteral Balloons: A Comparative in-Vitro Analysis

2005 ◽  
Vol 173 (4S) ◽  
pp. 315-316
Author(s):  
Kari Hendlin ◽  
Brynn Lund ◽  
Manoj Monga
Keyword(s):  
Vitro Analysis ◽  
In Vitro Analysis
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