scholarly journals An Abies procera-derived tetracyclic triterpene containing a steroid-like nucleus core and a lactone side chain attenuates in vitro survival of both Fasciola hepatica and Schistosoma mansoni

2018 ◽  
Vol 8 (3) ◽  
pp. 465-474 ◽  
Author(s):  
Helen L. Whiteland ◽  
Anand Chakroborty ◽  
Josephine E. Forde-Thomas ◽  
Alessandra Crusco ◽  
Alan Cookson ◽  
...  
Keyword(s):  
Schistosoma Mansoni ◽  
Fasciola Hepatica ◽  
Side Chain ◽  
Abies Procera ◽  
In Vitro Survival

Juvenile Fasciola hepatica are resistant to killing in vitro by free radicals compared with larvae of Schistosoma mansoni

2000 ◽  
Vol 22 (6) ◽  
pp. 287-295 ◽  
Author(s):  
David Piedrafita ◽  
Terry W. Spithill ◽  
John P. Dalton ◽  
Paul J. Brindley ◽  
Mark R. Sandeman ◽  
...  
Keyword(s):  
Free Radicals ◽  
Schistosoma Mansoni ◽  
Fasciola Hepatica

How anthelmintics help us to understand helminths

Parasitology ◽  
1985 ◽  
Vol 90 (4) ◽  
pp. 675-685 ◽  
Author(s):  
H. Vanden Bossche
Keyword(s):  
Molecular Weight ◽  
Schistosoma Mansoni ◽  
Mode Of Action ◽  
Liver Fluke ◽  
Fasciola Hepatica ◽  
Atp Synthesis ◽  
Aerobic Metabolism ◽  
Unexpected Finding

Study of the mode of action of the anti-parasitic ‘hydrogenophore’, closantel, revealed that mitochondria isolated from livers of untreated rats, 13 weeks after infection with Fasciola hepatica metacercariae, were uncoupled. This uncoupling of mitochondria might be induced by a product(s) excreted by the liver fluke. The lipid extractable product(s) binds to albumin and has a molecular weight between 500 and 1000 Daltons. A second unexpected finding indicates that closantel affects the motility of Schistosoma mansoni in vitro by interferin with mitochondrial ATP synthesis. The results obtained are suggestive of a role for aerobic metabolism in the generation of energy required for motility.

Antigenicity of a proteolytic enzyme of Fasciola hepatica

1988 ◽  
Vol 62 (3) ◽  
pp. 257-260 ◽  
Author(s):  
G. C. Coles ◽  
D. Rubano
Keyword(s):  
Molecular Weight ◽  
Schistosoma Mansoni ◽  
Western Blotting ◽  
Gel Electrophoresis ◽  
Proteolytic Enzyme ◽  
Fasciola Hepatica

ABSTRACTThe possibility was examined of using a haemoglobinase released during in vitro incubation of adult Fasciola hepatica for immunodiagnosis of fascioliasis. By SDS gel electrophoresis the enzyme appeared as two closely migrating bands with a molecular weight of approximately 27 000 daltons. After Western blotting the bands reacted with serum from rats infected with F. hepatica and mice infected with Schistosoma mansoni. The enzyme was therefore not a good antigen for immunodiagnosis.

Passive immunization of mice against Schistosoma mansoni with an IgM monoclonal antibody

Parasitology ◽  
1982 ◽  
Vol 84 (1) ◽  
pp. 83-91 ◽  
Author(s):  
M. A. Smith ◽  
J. A. Clegg ◽  
D. Snary ◽  
A. J. Trejdosiewicz
Keyword(s):  
Monoclonal Antibody ◽  
Schistosoma Mansoni ◽  
Fasciola Hepatica ◽  
Passive Immunization ◽  
Immune Serum ◽  
The Other ◽  
Passive Protection ◽  
Igm Antibody

SUMMARYTwo hybridomas secreting monoclonal IgM antibody to Schistosoma mansoni have been isolated following fusion of spleen cells from Balb/c mice immunized with living S. mansoni and NS1 myeloma cells. One monoclonal IgM antibody (WP66.4) mediated about the same level of passive protection against a challenge infection as immune serum from mice with a chronic S. mansoni infection. The other monoclonal antibody (WP66.2) did not give a significant level of passive protection. This result indicates that the effective monoclonal antibody recognizes an antigen which may be a valuable candidate for experimental vaccination. In vitro one monoclonal antibody (WP66.4) caused a much higher level of complement-dependent cytotoxicity than the other (WP66.2), suggesting a possible mechanism for the effect observed in vivo. With indirect immunofluorescence both monoclonal antibodies reacted with surface determinants on living S. mansoni schistosomula, adult worms and miracidia but these determinants were not detected on cercariae or lung schistosomula. Neither monoclonal antibody cross-reacted with S. haematobium schistosomula or Fasciola hepatica metacercariae, indicating a possible use for these reagents in differential diagnosis of S. mansoni infections.

In vitro schistosomicidal activity of Usnea steineri extract and its major constituent (+)-usnic acid against Schistosoma mansoni

Planta Medica ◽  
2012 ◽  
Vol 78 (11) ◽  
Author(s):  
AIO Salloum ◽  
R Lucarini ◽  
MG Tozatti ◽  
J Medeiros ◽  
MLA Silva ◽  
...  
Keyword(s):  
Schistosoma Mansoni ◽  
Usnic Acid ◽  
Major Constituent

Structure-Reactivity Relationships on Substrates and Inhibitors of the Lysine Deacylase Sirtuin 2 from Schistosoma Mansoni (SmSirt2)

2019 ◽  
Author(s):  
Daria Monaldi ◽  
Dante Rotili ◽  
Julien Lancelot ◽  
Martin Marek ◽  
Nathalie Wössner ◽  
...  
Keyword(s):  
Schistosoma Mansoni ◽  
Human Cancer ◽  
Egg Laying ◽  
Human Cancer Cells ◽  
Parasite Survival ◽  
Survival And Reproduction ◽  
Emergence Of Resistance ◽  
First Time ◽  
Parasitic Life Cycle

The only drug for treatment of Schistosomiasis is Praziquantel, and the possible emergence of resistance makes research on novel therapeutic agents necessary. Targeting of Schistosoma mansoni epigenetic enzymes, which regulate the parasitic life cycle, emerged as promising approach. Due to the strong effects of human Sirtuin inhibitors on parasite survival and reproduction, Schistosoma sirtuins were postulated as therapeutic targets. In vitro testing of synthetic substrates of S. mansoni Sirtuin 2 (SmSirt2) and kinetic experiments on a myristoylated peptide demonstrated lysine long chain deacylation as an intrinsic SmSirt2 activity for the first time. Focused in vitro screening of the GSK Kinetobox library and structure-activity relationships (SAR) of identified hits, led to the first SmSirt2 inhibitors with activity in the low micromolar range. Several SmSirt2 inhibitors showed potency against both larval schistosomes (viability) and adult worms (pairing, egg laying) in culture without general toxicity to human cancer cells.<br>

scholarly journals Imaging prior to radiotherapy impacts in-vitro survival

2020 ◽  
Vol 16 ◽  
pp. 138-143
Author(s):  
Peter L. Kench ◽  
Linda Rogers ◽  
Ana Esteves ◽  
Tina Gorjiara ◽  
Elizabeth Claridge Mackonis ◽  
...  
Keyword(s):  
In Vitro Survival

scholarly journals The soluble glutathione transferase superfamily: role of Mu class in triclabendazole sulphoxide challenge in Fasciola hepatica

2021 ◽  
Vol 120 (3) ◽  
pp. 979-991
Author(s):  
Rebekah B. Stuart ◽  
Suzanne Zwaanswijk ◽  
Neil D. MacKintosh ◽  
Boontarikaan Witikornkul ◽  
Peter M. Brophy ◽  
...  
Keyword(s):  
In Vitro Culture ◽  
Liver Fluke ◽  
Glutathione Transferase ◽  
Fasciola Hepatica ◽  
Affinity Purification ◽  
Economic Loss ◽  
Differential Abundance ◽  
Parasitic Helminths

AbstractFasciola hepatica (liver fluke), a significant threat to food security, causes global economic loss for the livestock industry and is re-emerging as a foodborne disease of humans. In the absence of vaccines, treatment control is by anthelmintics; with only triclabendazole (TCBZ) currently effective against all stages of F. hepatica in livestock and humans. There is widespread resistance to TCBZ and its detoxification by flukes might contribute to the mechanism. However, there is limited phase I capacity in adult parasitic helminths with the phase II detoxification system dominated by the soluble glutathione transferase (GST) superfamily. Previous proteomic studies have demonstrated that the levels of Mu class GST from pooled F. hepatica parasites respond under TCBZ-sulphoxide (TCBZ-SO) challenge during in vitro culture ex-host. We have extended this finding by exploiting a sub-proteomic lead strategy to measure the change in the total soluble GST profile (GST-ome) of individual TCBZ-susceptible F. hepatica on TCBZ-SO-exposure in vitro culture. TCBZ-SO exposure demonstrated differential abundance of FhGST-Mu29 and FhGST-Mu26 following affinity purification using both GSH and S-hexyl GSH affinity. Furthermore, a low or weak affinity matrix interacting Mu class GST (FhGST-Mu5) has been identified and recombinantly expressed and represents a new low-affinity Mu class GST. Low-affinity GST isoforms within the GST-ome was not restricted to FhGST-Mu5 with a second likely low-affinity sigma class GST (FhGST-S2) uncovered. This study represents the most complete Fasciola GST-ome generated to date and has supported the potential of subproteomic analyses on individual adult flukes.

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