Synergistic Cytotoxic Effect of a Novel mTOR/PI3K Dual Inhibitor PF-04691502 and Radiation Therapy on Neuroendocrine Tumor Cells

Unsatisfactory results of complex treatment for malignant brain tumors stimulate search of new effective methods of treatment. Radiation therapy is an integral part of the combined treatment but often does not influence lethally on resistant tumor cells. Thereby in recent decades there has been an active search for different modifiers, which can increase the sensitivity of tumors to chemotherapy and radiotherapy. One of the universal sensitizers is the local hyperthermia. Experimental data showed that the effect of high temperatures had both a direct damaging effect on tumor cells and a sensitizing effect. The literature review given in the article provides an overview of the existing methods of the local hyperthermia for brain tumors treatment.

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The Cytotoxic Effect of the Wild-Type Newcastle Disease Virus Strain on Tumor Cells in vitro

Cell and Tissue Biology ◽

10.1134/s1990519x20040094 ◽

2020 ◽

Vol 14 (4) ◽

pp. 243-249

Author(s):

E. V. Shekunov ◽

K. S. Yurchenko ◽

A. M. Shestopalov

Keyword(s):

Newcastle Disease Virus ◽

Tumor Cells ◽

Disease Virus ◽

Virus Strain ◽

Newcastle Disease ◽

Cytotoxic Effect ◽

Newcastle Disease Virus Strain ◽

Wild Type

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Bi-Functional Radiotheranostics of 188Re-Liposome-Fcy-hEGF for Radio- and Chemo-Therapy of EGFR-Overexpressing Cancer Cells

International Journal of Molecular Sciences ◽

10.3390/ijms22041902 ◽

2021 ◽

Vol 22 (4) ◽

pp. 1902 ◽

Cited By ~ 1

Author(s):

Yi-Shu Huang ◽

Wei-Chuan Hsu ◽

Chien-Hong Lin ◽

Sheng-Nan Lo ◽

Chu-Nian Cheng ◽

...

Keyword(s):

Fusion Protein ◽

Cancer Cells ◽

Tumor Cells ◽

Cytotoxic Effect ◽

Cytosine Deaminase ◽

Growth Factor Receptor ◽

Size Exclusion ◽

Specific Therapy ◽

Egfr Expression ◽

Epidermal Growth

Epidermal growth factor receptor (EGFR) specific therapeutics is of great importance in cancer treatment. Fcy-hEGF fusion protein, composed of yeast cytosine deaminase (Fcy) and human EGF (hEGF), is capable of binding to EGFR and enzymatically convert 5-fluorocytosine (5-FC) to 1000-fold toxic 5-fluorocuracil (5-FU), thereby inhibiting the growth of EGFR-expressing tumor cells. To develop EGFR-specific therapy, 188Re-liposome-Fcy-hEGF was constructed by insertion of Fcy-hEGF fusion protein onto the surface of liposomes encapsulating of 188Re. Western blotting, MALDI-TOF, column size exclusion and flow cytometry were used to confirm the conjugation and bio-activity of 188Re-liposome-Fcy-hEGF. Cell lines with EGFR expression were subjected to treat with 188Re-liposome-Fcy-hEGF/5-FC in the presence of 5-FC. The 188Re-liposome-Fcy-hEGF/5-FC revealed a better cytotoxic effect for cancer cells than the treatment of liposome-Fcy-hEGF/5-FC or 188Re-liposome-Fcy-hEGF alone. The therapeutics has radio- and chemo-toxicity simultaneously and specifically target to EGFR-expression tumor cells, thereby achieving synergistic anticancer activity.

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