Photon flux density and wavelength influence on growth, photosynthetic pigments and volatile organic compound accumulation in Aeollanthus suaveolens (Catinga-de-mulata) under in vitro conditions

Coconut kopyor is one of the most important commodities. One of the problems in coconut kopyor cultivation is grey leaf spot disease caused by Curvularia sp. Using endophytic bacteria is one of the control technique that is environmentally friendly. A total of 40 selected endophytic Arecaceaebacteria isolated from coconut kopyor, palm oil, aren, nibung and pejibaye were tested for their inhibitory ability to Curvularia sp.through antibiotic and volatile organic compound (VOC)test.The antibiotic test showed that thirty three endophytic bacteria isolates have inhibitory capacity againstCurvularia sp. in a range of inhibition from 4.4% to 86.6%. Isolates with the highest inhibition were EAKSS 502, EAKSS 520 and EAKSS 507. VOC test showed that EAPJN 216, EAKSS 532, EAAPN 225, EAAPN 506, EAAPN 507 and EAAPN 557 were produced VOC that suppressed the growth of Curvulariasp fungal colonies in a range from 92.27% to 97.21%. Based on the best combination of antibiotic and production of volatile organic compound test, there were four potential isolates to inhibit the growth of Curvulariasp. in vitro i.e. EAKSS 502, EAKSS 507, EAKPN 201 and EAPJN 216. Those isolates were molecularly identified as Serratia marcescensstrain PIGB81, Burkholderiasp. DOP Ma316, S. marcescensstrain RY21 and S. marcescensstrain LB21.The four isolates were isolated from different plants such oil palm, coconut kopyor and pejibaye.[Keywords: antibiotics,Burkholderia,malforma-tion, Serratia,suppression, volatile compound]AbstrakKelapa kopyor saat ini menjadi salah satu komoditas perkebunan yang penting. Salah satu masalah dalam pembudidayaan kelapa kopyor adalah serangan penyakit bercak kelabu yang disebabkan oleh cendawan Curvulariasp. Penggunaan bakteri endofit merupakan salah satu cara control yang ramah lingkungan.Sebanyak 40 isolat bakteri endofit asal tanaman Arecaceaediisolasi dari tanaman kelapa kopyor, kelapa sawit, aren, nibung dan pejibaye diujikan kemampuan penghambatannya terhadap Curvulariasp. melalui uji antibiosis dan uji produksi senyawa organik volatil (VOC). Uji antibiosis menunjukkan se-banyak 33 isolat bakteri endofit menunjukkan daya penghambatan terhadap cendawan Curvulariasp. dengan kisaran 4,4%-86,6%. Penghambatan terbesar yakni isolat EAKSS 502, EAKSS 520 dan isolat EAKSS 507. Pengujian produksi senyawa organik volatil menunjukkan EAPJN 216, EAKSS 532, EAAPN 225, EAAPN 506, EAAPN 507 dan EAAPN 557 menghasilkan komponen volatil organik yang menekan pertumbuhan koloni cendawan Curvulariasp. pada kisaran 92,27%- 97,21%. Berdasarkan kombinasi data pengujian antibioisis dan produksi senyawa organik volatilterdapat 4 isolat bakteri endofit yang berpotensi menghambat perkembangan Curvulariasp. yaitu isolat EAKSS 502, EAKSS 507, EAKPN 201 dan EAPJN 216.Hasil identifikasi secara molekuler ke empat isolat tersebut berturut-turut adalah Serratia marcescens strain PIGB81,Burkholderia sp. DOP Ma316,S. marcescens strain RY21danS. marcescens strain LB21. Keempat isolat tersebut diisolasi daritanaman yang berbeda yakni kelapa sawit, kelapa kopyor dan pejibaye.[Kata kunci: antibiotik, Burkholderia,malformasi, penghambatan, Serratia,komponen volatil].

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Shoot Apex Sugars in Relation to Long-Day Induction of Flowering in Lolium temulentum L

Functional Plant Biology ◽

10.1071/pp9910121 ◽

1991 ◽

Vol 18 (2) ◽

pp. 121 ◽

Cited By ~ 11

Author(s):

RW King ◽

LT Evans

Keyword(s):

Flux Density ◽

Shoot Apex ◽

Photon Flux ◽

Photon Flux Density ◽

Sucrose Content ◽

Inflorescence Development ◽

Lolium Temulentum ◽

Long Day ◽

Floral Evocation

Inflorescence initiation in Lolium temulentum is induced by a single long day with a photoperiod extension of 16 h under low photon flux density (12 μmol PAR m-2 s-1) from incandescent lamps. Under these conditions the content of sucrose, the predominant free sugar in the shoot apex, fluctuates diurnally in the same way as in short day apices. There was no evidence of a greater apical sucrose content at any time during the long day or in the following period of high irradiance when floral evocation occurs. Thereafter, however, the diurnal fluctuation in apical sucrose content became more pronounced. Increasing the sugar supply to the apex by raising the photon flux density during the daily light period did not lead to flowering of non-induced plants; nor did the high contents of apical sugars reached in apices cultured in vitro on 5% sucrose medium. By contrast, when apices were excised after receipt of the floral stimulus from long day leaves, increase in the sugar content enhanced inflorescence development in vitro, this response being most pronounced after the inflorescences were initiated. Thus, floral evocation in L. temulentum does not require an increase in the content of sucrose at the apex although inflorescence development is highly responsive to it. When photoperiodic extensions with incandescent or fluorescent lamps were compared for their effects on apical sugars and flowering response, there was no interaction between light quality and photon flux density. Thus the shoot apex response to the low irradiance, photoperiodic time-measurement processes of leaves is distinct from the apical response to sugar supply. In Lolium temulentum floral evocation is controlled by the photoperiodic processes, the response to which is amplified by high sugar supplies but not replaced as it is in Sinapis alba.

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Effect of the Difference between Photoperiod and Dark Period Temperatures, and Photosynthetic Photon Flux Density on the Shoot Length and Growth of Potato Plantlets In Vitro.

Engei Gakkai zasshi ◽

10.2503/jjshs.61.93 ◽

1992 ◽

Vol 61 (1) ◽

pp. 93-98 ◽

Cited By ~ 14

Author(s):

Toyoki Kozai ◽

Sunao Kushihashi ◽

Chieri Kubota ◽

Kazuhiro Fujiwara

Keyword(s):

Flux Density ◽

Dark Period ◽

Photosynthetic Photon Flux Density ◽

Shoot Length ◽

Photon Flux ◽

Photon Flux Density ◽

Photosynthetic Photon Flux ◽

The Difference ◽

Potato Plantlets

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The physiology of Cymbidium plantlets cultured in vitro under conditions of high carbon dioxide and low photosynthetic photon flux density

The Journal of Horticultural Science and Biotechnology ◽

10.1080/14620316.1999.11511165 ◽

1999 ◽

Vol 74 (5) ◽

pp. 632-638 ◽

Cited By ~ 8

Author(s):

M. Tanaka ◽

D. C. H. Yap ◽

C. K. Y. Ng ◽

C. S. Hew

Keyword(s):

Carbon Dioxide ◽

Flux Density ◽

Photosynthetic Photon Flux Density ◽

Photon Flux ◽

Photon Flux Density ◽

High Carbon ◽

Photosynthetic Photon Flux ◽

High Carbon Dioxide

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Air quality within the incubator: will volatile organic compound (VOC) filters make a difference in in vitro fertilization?

Fertility and Sterility ◽

10.1016/s0015-0282(03)01653-4 ◽

2003 ◽

Vol 80 ◽

pp. 260 ◽

Cited By ~ 3

Author(s):

Homer L. Higdon ◽

Jennifer E. Graves ◽

Dawn W. Blackhurst ◽

William R. Boone

Keyword(s):

Air Quality ◽

In Vitro Fertilization ◽

Organic Compound ◽

Volatile Organic Compound ◽

Vitro Fertilization ◽

Volatile Organic

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In vitro volatile organic compound profiling using GC×GC-TOFMS to differentiate bacteria associated with lung infections: a proof-of-concept study

Journal of Breath Research ◽

10.1088/1752-7155/10/2/026008 ◽

2016 ◽

Vol 10 (2) ◽

pp. 026008 ◽

Cited By ~ 35

Author(s):

K D Nizio ◽

K A Perrault ◽

A N Troobnikoff ◽

M Ueland ◽

S Shoma ◽

...

Keyword(s):

Organic Compound ◽

Volatile Organic Compound ◽

Proof Of Concept ◽

Concept Study ◽

Volatile Organic ◽

Lung Infections

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Critical Review of Volatile Organic Compound Analysis in Breath and In Vitro Cell Culture for Detection of Lung Cancer

Metabolites ◽

10.3390/metabo9030052 ◽

2019 ◽

Vol 9 (3) ◽

pp. 52 ◽

Cited By ~ 26

Author(s):

Zhunan Jia ◽

Abhijeet Patra ◽

Viknish Kutty ◽

Thirumalai Venkatesan

Keyword(s):

Lung Cancer ◽

Organic Compound ◽

Volatile Organic Compound ◽

Rapid Development ◽

Lung Cancer Screening ◽

Breath Analysis ◽

Exhaled Breath ◽

Exhaled Breath Analysis ◽

Volatile Organic

Breath analysis is a promising technique for lung cancer screening. Despite the rapid development of breathomics in the last four decades, no consistent, robust, and validated volatile organic compound (VOC) signature for lung cancer has been identified. This review summarizes the identified VOC biomarkers from both exhaled breath analysis and in vitro cultured lung cell lines. Both clinical and in vitro studies have produced inconsistent, and even contradictory, results. Methodological issues that lead to these inconsistencies are reviewed and discussed in detail. Recommendations on addressing specific issues for more accurate biomarker studies have also been made.

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