Protective and anti-inflammatory effect of a traditional Chinese medicine, Xia-Bai-San, by modulating lung local cytokine in a murine model of acute lung injury

2006 ◽  
Vol 6 (9) ◽  
pp. 1506-1514 ◽  
Author(s):  
Chia-Chou Yeh ◽  
Chih-Che Lin ◽  
Shulhn-Der Wang ◽  
Yueh-Sheng Chen ◽  
Bai-Horng Su ◽  
...  
Keyword(s):  
Acute Lung Injury ◽  
Chinese Medicine ◽  
Traditional Chinese Medicine ◽  
Lung Injury ◽  
Murine Model ◽  
Anti Inflammatory ◽  
Local Cytokine ◽  
Inflammatory Effect

Cannabidiol produces a long-term anti-inflammatory effect in a murine model of acute lung injury

2011 ◽  
Vol 25 ◽  
pp. S210 ◽  
Author(s):  
A. Ribeiro ◽  
V. Ferraz-de-Paula ◽  
M.L. Pinheiro ◽  
W.M. Quinteiro-Filho ◽  
A.T. Akamine ◽  
...  
Keyword(s):  
Acute Lung Injury ◽  
Lung Injury ◽  
Murine Model ◽  
Anti Inflammatory ◽  
Inflammatory Effect

scholarly journals Nicotine Exerts an Anti-inflammatory Effect in a Murine Model of Acute Lung Injury

Inflammation ◽  
2010 ◽  
Vol 34 (4) ◽  
pp. 231-237 ◽  
Author(s):  
Jon Mabley ◽  
Sevelanne Gordon ◽  
Pal Pacher
Keyword(s):  
Acute Lung Injury ◽  
Lung Injury ◽  
Murine Model ◽  
Anti Inflammatory ◽  
Inflammatory Effect

scholarly journals THE NOVEL INOSINE ANALOGUE INO-2002 EXERTS AN ANTI-INFLAMMATORY EFFECT IN A MURINE MODEL OF ACUTE LUNG INJURY

Shock ◽  
2009 ◽  
Vol 32 (3) ◽  
pp. 258-262 ◽  
Author(s):  
Jon G. Mabley ◽  
Pal Pacher ◽  
Kanneganti G.K. Murthy ◽  
William Williams ◽  
Garry J. Southan ◽  
...  
Keyword(s):  
Acute Lung Injury ◽  
Lung Injury ◽  
Murine Model ◽  
The Novel ◽  
Anti Inflammatory ◽  
Inflammatory Effect

Vanillin protects lipopolysaccharide-induced acute lung injury by inhibiting ERK1/2, p38 and NF-κB pathway

2019 ◽  
Vol 11 (16) ◽  
pp. 2081-2094 ◽  
Author(s):  
Tingting Guo ◽  
Zhenzhong Su ◽  
Qi Wang ◽  
Wei Hou ◽  
Junyao Li ◽  
...  
Keyword(s):  
Acute Lung Injury ◽  
Lung Injury ◽  
Western Blot ◽  
Lung Inflammation ◽  
Macrophage Activation ◽  
Myeloperoxidase Activity ◽  
Histopathological Changes ◽  
Tnf Α ◽  
Anti Inflammatory ◽  
Inflammatory Effect

Aim: Thus far, the anti-inflammatory effect of vanillin in acute lung injury (ALI) has not been studied. This study aimed to investigate the effect of vanillin in lipopolysaccharide (LPS)-induced ALI. Results & methodology: Our study detected the anti-inflammatory effects of vanillin by ELISA and western blot, respectively. Pretreatment of mice with vanillin significantly attenuated LPS-stimulated lung histopathological changes, myeloperoxidase activity and expression levels of proinflammatory cytokines by inhibiting the phosphorylation activities of ERK1/2, p38, AKT and NF-κB p65. In addition, vanillin inhibited LPS-induced TNF-α and IL-6 expression in RAW264.7 cells via ERK1/2, p38 and NF-κB signaling. Conclusion: Vanillin can inhibit macrophage activation and lung inflammation, which suggests new insights for clinical treatment of ALI.

scholarly journals Anti-inflammatory effects of triptolide by inhibiting the NF-κB signalling pathway in LPS-induced acute lung injury in a murine model

2014 ◽  
Vol 10 (1) ◽  
pp. 447-452 ◽  
Author(s):  
XIAN WANG ◽  
LEI ZHANG ◽  
WEI DUAN ◽  
BIN LIU ◽  
PING GONG ◽  
...  
Keyword(s):  
Acute Lung Injury ◽  
Lung Injury ◽  
Murine Model ◽  
Signalling Pathway ◽  
Anti Inflammatory

scholarly journals A Standardized Traditional Chinese Medicine Preparation Named Yejuhua Capsule Ameliorates Lipopolysaccharide-Induced Acute Lung Injury in Mice via Downregulating Toll-Like Receptor 4/Nuclear Factor-κB

2015 ◽  
Vol 2015 ◽  
pp. 1-15 ◽  
Author(s):  
Chu-Wen Li ◽  
Zhi-Wei Chen ◽  
Xiao-Li Wu ◽  
Zhao-Xiao Ning ◽  
Zu-Qing Su ◽  
...  
Keyword(s):  
Acute Lung Injury ◽  
Chinese Medicine ◽  
Traditional Chinese Medicine ◽  
Lung Injury ◽  
Therapeutic Drug ◽  
Nuclear Factor ◽  
Protective Effects ◽  
Toll Like Receptor ◽  
Toll Like Receptor 4 ◽  
Traditional Chinese Medicine Preparation

A standardized traditional Chinese medicine preparation named Yejuhua capsule (YJH) has been clinically used in treatments of various acute respiratory system diseases with high efficacy and low toxicity. In this study, we were aiming to evaluate potential effects and to elucidate underlying mechanisms of YJH against lipopolysaccharide- (LPS-) induced acute lung injury (ALI) in mice. Moreover, the chemical analysis and chromatographic fingerprint study were performed for quality evaluation and control of this drug. ALI was induced by intratracheal instillation of LPS (5 mg/kg) into the lung in mice and dexamethasone (5 mg/kg, p.o.) was used as a positive control drug. Results demonstrated that pretreatments with YJH (85, 170, and 340 mg/kg, p.o.) effectively abated LPS-induced histopathologic changes, attenuated the vascular permeability enhancement and edema, inhibited inflammatory cells migrations and protein leakages, suppressed the ability of myeloperoxidase, declined proinflammatory cytokines productions, and downregulated activations of nuclear factor-κB (NF-κB) and expressions of toll-like receptor 4 (TLR4). This study demonstrated that YJH exerted potential protective effects against LPS-induced ALI in mice and supported that YJH was a potential therapeutic drug for ALI in clinic. And its mechanisms were at least partially associated with downregulations of TLR4/NF-κB pathways.

scholarly journals Anti-Inflammatory Effects of Huberia peruviana Cogn. Methanol Extract by Inhibiting Src Activity in the NF-κB Pathway

Plants ◽  
2021 ◽  
Vol 10 (11) ◽  
pp. 2335
Author(s):  
Seung A Kim ◽  
Chae Young Lee ◽  
Ankita Mitra ◽  
Haeyeop Kim ◽  
Byoung Young Woo ◽  
...  
Keyword(s):  
Acute Lung Injury ◽  
Lung Injury ◽  
Western Blotting ◽  
Methanol Extract ◽  
Luciferase Assay ◽  
Luciferase Reporter ◽  
No Production ◽  
In Vivo Models ◽  
Anti Inflammatory ◽  
Inflammatory Effect

There is a growing need to develop anti-inflammatory drugs to regulate inflammatory responses. An extract of Huberia peruviana Cogn. had the best inhibitory effect on nitric oxide (NO) production in screening process undertaken in our laboratory. However, the anti-inflammatory effect of Huberia peruviana Cogn. methanol extract (Hp-ME) has not been studied. In this study, the anti-inflammatory effect of Hp-ME was assessed by using an NO assay, RT-PCR, luciferase reporter gene activity assay, western blotting assay, HCl/EtOH-induced acute gastritis model, and LPS-induced acute lung injury model. The phytochemical components of Hp-ME were determined through LC-MS/MS analysis. When RAW264.7 and HEK293T cells were treated with Hp-ME, NO production was decreased dose-dependently without cytotoxicity and the mRNA levels of iNOS, COX-2, and TNF-α were decreased. In a luciferase assay, the activity of transcription factors, NF-κB in TRIF or MyD88-overexpressing HEK293T cells was extremely reduced by Hp-ME. The western blotting analysis indicated that Hp-ME has anti-inflammatory effects by inhibiting the phosphorylation of Src. Hp-ME showed anti-inflammatory effects on in vivo models of HCl/EtOH-induced gastritis and LPS-induced acute lung injury. LC-MS/MS revealed that Hp-ME contains several anti-inflammatory flavonoids. The final findings of this study imply that Hp-ME could be used as an anti-inflammatory drug in several inflammatory diseases.

scholarly journals Sphingosine Kinase 1 Plays an Important Role in Atorvastatin-Mediated Anti-Inflammatory Effect against Acute Lung Injury

2021 ◽  
Vol 2021 ◽  
pp. 1-14
Author(s):  
Lan Wu ◽  
Yan Cheng ◽  
Shunxiang Peng ◽  
Wensheng Zhang ◽  
Chaoxiong Zhang
Keyword(s):  
Acute Lung Injury ◽  
Lung Injury ◽  
Sphingosine Kinase ◽  
Sphingosine Kinase 1 ◽  
Cyclic Stretching ◽  
High Tidal Volume Ventilation ◽  
Volume Ventilation ◽  
Anti Inflammatory ◽  
Endothelial Junction ◽  
Inflammatory Effect

Atorvastatin is a 3-hydroxy-3-methylglutaryl coenzyme A reductase (HMG-CoA reductase) inhibitor and inhibits cholesterol synthesis. Recently, atorvastatin also showed anti-inflammatory effect in acute lung injury, ameliorating pulmonary gas-blood exchanging function. Sphingosine kinase 1 plays a central role in endothelial (EC) cytoskeleton rearrangement and EC barrier integrity regulation. In this study, the role of sphingosine kinase 1 in atorvastatin anti-inflammatory effect against acute lung injury was investigated. Both wild-type (WT) and SphK1-/- mice were challenged with high tidal volume ventilation (40 ml/kg body weight, 65 breathing/min, 4 hours). The acute lung injury was evaluated and the mechanisms were explored. In WT mice, atorvastatin treatment significantly decreased acute lung injury responding to high tidal volume ventilation (HT), including protein, cellular infiltration, and cytokine releasing; comparing to WT mice, SphK1-/- mice showed significantly worsen pulmonary injuries on HT model. Moreover, the atorvastatin-mediated anti-inflammatory effect was diminished in SphK1-/- mice. To further confirm the role of SphK1 in VILI, we then compared the inflammatory response of endothelial cells that were isolated from WT and SphK1-/- mice to cyclic stretching. Similarly, atorvastatin significantly decreased cytokine generation from WT EC responding to cyclic stretching. Atorvastatin also significantly preserved endothelial junction integrity in WT EC against thrombin challenge. However, the inhibitory effect of atorvastatin on cytokine generation induced by cyclic stretching was abolished on SphK1-/- mice EC. The endothelial junction integrity effects of atorvastatin also diminished on SphK1-/- mouse EC. Signal analysis indicated that atorvastatin inhibited JNK activation induced by cyclic stretch. SphK1 knockout also blocked atorvastatin-mediated VE-cadherin junction enhancement. In summary, by inhibition of MAPK activity and maintenance of EC junction homeostasis, SphK1 plays a critical role in atorvastatin-mediated anti-inflammatory effects in both cellular and in vivo model. This study also offers an insight into mechanical stress-mediated acute lung injury and potential therapy in the future.

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