Human growth factor and cytokine skin cream promotes neocollagenesis in vivo

Abstract The cells of hematopoietic and vascular endothelial cell lineages are believed to share a common precursor, termed hemangioblast. However, the existence of a growth factor acting relatively specifically on hemangioblasts remains unclear. Here we report the identification of hemangiopoietin (HAPO), a novel growth factor acting on both hematopoietic and endothelial cell lineages. In vitro in the human system, recombinant human HAPO (rhHAPO) significantly stimulated the proliferation and hematopoietic and/or endothelial differentiation of human bone marrow mononuclear cells and of purified CD34+, CD133+, kinase domain receptor-positive (KDR+), or CD34+/KDR+ cell populations. In the murine system, rhHAPO stimulated the proliferation of long-term culture-initiating cells (LTC-ICs) as well as CD34+ and stem cell antigen-1 (Sca-1+) cell subsets. In vivo, subcutaneous injection of rhHAPO into normal mice resulted in a significant increase in bone marrow hematopoietic cells. Furthermore, irradiated mice injected with rhHAPO had an enhanced survival rate and accelerated hematopoiesis. Our data suggest that HAPO is a novel growth factor acting on the primitive cells of both hematopoietic and endothelial cell lineages and that HAPO may have a clinical potential in the treatment of various cytopenias and radiation injury and in the expansion of hematopoietic and endothelial stem/progenitor cells. (Blood. 2004;103:4449-4456)

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Intestinal response to growth factors administered alone or in combination with human [Gly2]glucagon-like peptide 2

AJP Gastrointestinal and Liver Physiology ◽

10.1152/ajpgi.1997.273.6.g1252 ◽

1997 ◽

Vol 273 (6) ◽

pp. G1252-G1262 ◽

Cited By ~ 19

Author(s):

Daniel J. Drucker ◽

Lorraine Deforest ◽

Patricia L. Brubaker

Keyword(s):

Growth Factors ◽

Growth Factor ◽

Large Bowel ◽

Intestinal Adaptation ◽

Human Growth ◽

Intestinal Growth ◽

Cellular Targets ◽

Igf I ◽

Glucagon Like Peptide

The control of intestinal epithelial growth is regulated by interactions of growth factors in various cellular compartments of the small and large bowel. Little information is available on the intestinal growth response to combinations of growth factors. We studied the intestinotrophic properties of a dipeptidyl peptidase IV resistant glucagon-like peptide 2 (GLP-2) analog, human [Gly2]GLP-2 (h[Gly2]GLP-2), as well as of epidermal growth factor (EGF), long [Arg3]insulin-like growth factor I (LR3IGF-I), [Gly1]IGF-II, and human growth hormone (hGH), administered by subcutaneous injection alone or in combination in mice. At the doses tested, h[Gly2]GLP-2 was the most potent agent for increasing small and large bowel mass. Mice treated with h[Gly2]GLP-2 and either GH or IGF-I exhibited greater increases in histological parameters of small intestinal growth than did mice treated with h[Gly2]GLP-2 alone. Administration of all five growth factors together induced significant increases in crypt plus villus height and in small and large bowel length and weight. The results of these experiments define regional differences in both the cellular targets and relative activities of intestinotrophic molecules and raise the possibility that selective growth factor combinations may be useful for enhancement of intestinal adaptation in vivo.

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Clinical, histologic, and ultrastructural changes after use of human growth factor and cytokine skin cream for the treatment of skin rejuvenation

Journal of Cosmetic and Laser Therapy ◽

10.1080/14764170701885392 ◽

2008 ◽

Vol 10 (2) ◽

pp. 104-109 ◽

Cited By ~ 17

Author(s):

Mussarrat Hussain ◽

Robert Phelps ◽

David J. Goldberg

Keyword(s):

Growth Factor ◽

Human Growth ◽

Ultrastructural Changes ◽

Skin Rejuvenation ◽

Skin Cream

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Evaluation of a Collagen-Hyaluronate Bilayer Matrix for Bone and Cartilage Repair

MRS Proceedings ◽

10.1557/proc-662-ll1.9 ◽

2000 ◽

Vol 662 ◽

Author(s):

Lin-Shu Liu ◽

Andrea Thompson ◽

Robin Daverman ◽

James W. Poser ◽

Robert C. Spiro

Keyword(s):

Growth Factor ◽

Type I Collagen ◽

Human Growth ◽

Type Ii Collagen ◽

Cartilage Tissue ◽

Type I ◽

Collagen Layer ◽

And Cartilage

AbstractWe have developed a novel bilayer matrix composed of a porous type I collagen layer that transitions into a hyaluronate gel layer. This study evaluates the potential of the bilayer matrix to support the in vitro and in vivo formation of both bone and cartilage tissue. In the presence of recombinant human growth and differentiation factor-5, fetal rat calvarial cells cultured in the HA layer grew in a round, aggregated, chondrocyte-like morphology, while those in the collagen layer grew flattened and spread. Biochemical analysis demonstrated that cells in the collagen layer expressed higher levels of alkaline phosphatase activity, and lower levels of sulfated glycosaminoglycans and type II collagen when compared to cells in the HA layer. Intramuscular implants of the bilayer matrix with growth factor retrieved at 28 days revealed the presence of bone and cartilage tissue in the collagen and hyaluronate layers, respectively. These results demonstrate that the differentiation of cells in response to a single growth factor can be guided by specific compositional changes of the extracellular matrix.

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