scholarly journals Cost-Effective Addition of High-Throughput Colony Picking Capability to a Standard Liquid-Handling Platform

2009 ◽  
Vol 14 (1) ◽  
pp. 22-26 ◽  
Author(s):  
T HARTLEY ◽  
C STEWART ◽  
R STEWART ◽  
D MUNROE
Keyword(s):  
High Throughput ◽  
Cost Effective ◽  
Liquid Handling

scholarly journals A Cost-Effective Solution to Reduce Dead Volume of a Standard Dispenser System by a Factor of 5

2006 ◽  
Vol 11 (4) ◽  
pp. 407-412 ◽  
Author(s):  
Christian Bergsdorf ◽  
Nadine Gewiese ◽  
Andreas Stolz ◽  
Rainer Mann ◽  
Karsten Parczyk ◽  
...  
Keyword(s):  
High Throughput ◽  
High Throughput Screening ◽  
Cost Effective ◽  
Dead Volume ◽  
Liquid Handling ◽  
Accuracy And Precision ◽  
Handling Device ◽  
Maximum Benefit ◽  
To Receive ◽  
Development Laboratory

A key trend in high-throughput screening is assay miniaturization to control reagent costs and increase throughput. For this purpose, liquid-handling devices are used that transfer nano-to low-microliter volumes into all currently used microtiter well plates. One drawback of many available dispenser and pipetting systems are high dead volumes. Therefore, the authors were looking for an easy and simple solution to modify their standard liquid-handling device, PerkinElmer’s FlexDrop™ Precision IV, allowing for a dead volume reduction to receive maximum benefit from miniaturized assay formats. Internal reservoirs were developed and constructed by Schering’s Technical Development Laboratory (TDL), which are directly connected to the dispenser banks of FlexDrop™ without tubing. Using these newly built reservoirs, the dead volume was decreased by a factor of 5 in comparison to the manufacturer’s reservoirs without compromising liquid-handling parameters such as accuracy and precision. The modified system displayed a high robustness and reliability under routine high-throughput screening conditions.

scholarly journals A fully automated pipeline for the dynamic at‐line morphology analysis of microscale Aspergillus cultivation

2021 ◽  
Vol 8 (1) ◽  
Author(s):  
Roman Jansen ◽  
Kira Küsters ◽  
Holger Morschett ◽  
Wolfgang Wiechert ◽  
Marco Oldiges
Keyword(s):  
Filamentous Fungi ◽  
High Throughput ◽  
Automated Image Analysis ◽  
Height Velocity ◽  
Pellet Size ◽  
Application Study ◽  
Liquid Handling ◽  
Morphology Analysis ◽  
Development And Utilization ◽  
Increasing Demand

Abstract Background Morphology, being one of the key factors influencing productivity of filamentous fungi, is of great interest during bioprocess development. With increasing demand of high-throughput phenotyping technologies for fungi due to the emergence of novel time-efficient genetic engineering technologies, workflows for automated liquid handling combined with high-throughput morphology analysis have to be developed. Results In this study, a protocol allowing for 48 parallel microbioreactor cultivations of Aspergillus carbonarius with non-invasive online signals of backscatter and dissolved oxygen was established. To handle the increased cultivation throughput, the utilized microbioreactor is integrated into a liquid handling platform. During cultivation of filamentous fungi, cell suspensions result in either viscous broths or form pellets with varying size throughout the process. Therefore, tailor-made liquid handling parameters such as aspiration/dispense height, velocity and mixing steps were optimized and validated. Development and utilization of a novel injection station enabled a workflow, where biomass samples are automatically transferred into a flow through chamber fixed under a light microscope. In combination with an automated image analysis concept, this enabled an automated morphology analysis pipeline. The workflow was tested in a first application study, where the projected biomass area was determined at two different cultivation temperatures and compared to the microbioreactor online signals. Conclusions A novel and robust workflow starting from microbioreactor cultivation, automated sample harvest and processing via liquid handling robots up to automated morphology analysis was developed. This protocol enables the determination of projected biomass areas for filamentous fungi in an automated and high-throughput manner. This measurement of morphology can be applied to describe overall pellet size distribution and heterogeneity.

scholarly journals HiSpike Method for High-Throughput Cost Effective Sequencing of the SARS-CoV-2 Spike Gene

2022 ◽  
Vol 8 ◽  
Author(s):  
Ephraim Fass ◽  
Gal Zizelski Valenci ◽  
Mor Rubinstein ◽  
Paul J. Freidlin ◽  
Shira Rosencwaig ◽  
...  
Keyword(s):  
Real Time ◽  
High Throughput ◽  
Cost Effective ◽  
Developed Countries ◽  
Spike Protein ◽  
Medical Community ◽  
Effective Vaccine ◽  
Gene Variants ◽  
Spike Gene ◽  
Field Samples

The changing nature of the SARS-CoV-2 pandemic poses unprecedented challenges to the world's health systems. Emerging spike gene variants jeopardize global efforts to produce immunity and reduce morbidity and mortality. These challenges require effective real-time genomic surveillance solutions that the medical community can quickly adopt. The SARS-CoV-2 spike protein mediates host receptor recognition and entry into the cell and is susceptible to generation of variants with increased transmissibility and pathogenicity. The spike protein is the primary target of neutralizing antibodies in COVID-19 patients and the most common antigen for induction of effective vaccine immunity. Tight monitoring of spike protein gene variants is key to mitigating COVID-19 spread and generation of vaccine escape mutants. Currently, SARS-CoV-2 sequencing methods are labor intensive and expensive. When sequence demands are high sequencing resources are quickly exhausted. Consequently, most SARS-CoV-2 strains are sequenced in only a few developed countries and rarely in developing regions. This poses the risk that undetected, dangerous variants will emerge. In this work, we present HiSpike, a method for high-throughput cost effective targeted next generation sequencing of the spike gene. This simple three-step method can be completed in < 30 h, can sequence 10-fold more samples compared to conventional methods and at a fraction of their cost. HiSpike has been validated in Israel, and has identified multiple spike variants from real-time field samples including Alpha, Beta, Delta and the emerging Omicron variants. HiSpike provides affordable sequencing options to help laboratories conserve resources for widespread high-throughput, near real-time monitoring of spike gene variants.

Cost effective microsatellite isolation and genotyping by high throughput sequencing

2019 ◽  
Vol 47 (2) ◽  
pp. 190 ◽  
Author(s):  
Henrik Krehenwinkel ◽  
Susanne Meese ◽  
Christoph Mayer ◽  
Jasmin Ruch ◽  
Jutta Schneider ◽  
...  
Keyword(s):  
High Throughput ◽  
High Throughput Sequencing ◽  
Cost Effective ◽  
Microsatellite Isolation

scholarly journals Review: Cost-Effective Unmanned Aerial Vehicle (UAV) Platform for Field Plant Breeding Application

2020 ◽  
Vol 12 (6) ◽  
pp. 998 ◽  
Author(s):  
GyuJin Jang ◽  
Jaeyoung Kim ◽  
Ju-Kyung Yu ◽  
Hak-Jin Kim ◽  
Yoonha Kim ◽  
...  
Keyword(s):  
Plant Breeding ◽  
Unmanned Aerial Vehicle ◽  
High Throughput ◽  
New Technologies ◽  
Cost Effective ◽  
New Wave ◽  
Breeding Programs ◽  
Modern Agriculture ◽  
Aerial Vehicle ◽  
High Throughput Phenotyping

Utilization of remote sensing is a new wave of modern agriculture that accelerates plant breeding and research, and the performance of farming practices and farm management. High-throughput phenotyping is a key advanced agricultural technology and has been rapidly adopted in plant research. However, technology adoption is not easy due to cost limitations in academia. This article reviews various commercial unmanned aerial vehicle (UAV) platforms as a high-throughput phenotyping technology for plant breeding. It compares known commercial UAV platforms that are cost-effective and manageable in field settings and demonstrates a general workflow for high-throughput phenotyping, including data analysis. The authors expect this article to create opportunities for academics to access new technologies and utilize the information for their research and breeding programs in more workable ways.

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