Antibacterial activity of silver nanoparticles (AgNP) confined to mesostructured silica against methicillin-resistant Staphylococcus aureus (MRSA)

2018 ◽  
Vol 747 ◽  
pp. 1-7 ◽  
Author(s):  
Chi-Sheng Chien ◽  
Chao-Jen Lin ◽  
Chih-Jung Ko ◽  
Sung-Pin Tseng ◽  
Chi-Jen Shih
Keyword(s):  
Staphylococcus Aureus ◽  
Silver Nanoparticles ◽  
Antibacterial Activity ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Methicillin Resistant ◽  
Mesostructured Silica

scholarly journals Antibacterial Activity and Acute Toxicity Testing of Biosynthesized Silver Nanoparticles against Methicilin-Resistant Staphylococcus aureus and Pseudomonas aeruginosa

2021 ◽  
Author(s):  
Ifeyomi Wilfred Olobayotan ◽  
Bukola Catherine Akin-Osanaiye ◽  
Olukemi A. Onuh
Keyword(s):  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Silver Nanoparticles ◽  
Antibacterial Activity ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Toxicity Testing ◽  
Therapeutic Agents ◽  
Methicillin Resistant ◽  
Colony Forming Units ◽  
Test Organisms

Antibacterial activity of biosynthesized silver nanoparticles was studied using the macrobroth dilution technique. The silver nanoparticles was significantly active (p > 0.05) against the test organisms at an extract concentration of 75 µg/ml. Concentrations ≤ 50 µg/ml were not as effective as the colony forming units at this concentration, 1.61 x 106 for methicillin-resistant Staphylococcus aureus and concentrations ≤ 25 µg/ml 1.45 x 106 for Pseudomonas aeruginosa respectively, were about the same range as the colony forming units of the controls. The silver nanoparticles inhibited Methicillin-Resistant S. aureus more (MIC of 75 µg/ml and MBC of 100 µg/ml) than they inhibited P. aeruginosa (both MIC and MBC was 100 µg/ml). The LD50 of the synthesized silver nanoparticles after oral administration was seen to be greater than 5000 mg/kg body weight and is therefore thought to be safe. This study supports the use of silver nanoparticles as therapeutic agents. KEYWORDS: Nanoparticles, Biosynthesis, Inhibition, Therapeutic agents, Macroboth Technique

scholarly journals Augmented antibacterial activity of ampicillin with silver nanoparticles against methicillin-resistant Staphylococcus aureus (MRSA)

2018 ◽  
Vol 72 (1) ◽  
pp. 50-53 ◽  
Author(s):  
Priyanka Surwade ◽  
Christopher Ghildyal ◽  
Chase Weikel ◽  
Todd Luxton ◽  
Derek Peloquin ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Silver Nanoparticles ◽  
Antibacterial Activity ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Methicillin Resistant

scholarly journals Highly Antibacterial Activity Against Methicillin-resistant Staphylococcus aureus of Silver Nanoparticles Synthesized by Citrus maxima Peel Extract

2021 ◽  
Author(s):  
Thu Ha Bui ◽  
Ngoc Dai Nghia Tran ◽  
Phung Anh Nguyen ◽  
Nhat Linh Duong ◽  
Van Minh Nguyen ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Silver Nanoparticles ◽  
Antibacterial Activity ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Average Diameter ◽  
Reducing Agent ◽  
Face Centered Cubic ◽  
Methicillin Resistant ◽  
Vis Spectroscopy ◽  
Citrus Maxima

Abstract A cost-effective and green technique was performed for the synthesis of silver nanoparticles (AgNPs) from a plant resource using Citrus maxima peel (CMP) extract as a reducing agent. The formation of AgNPs was confirmed by UV-Vis Spectroscopy at the wavelength range of 400−500 nm. The optimized conditions for the AgNPs synthesis using CMP extract as a reducing agent were determined. At these conditions, the X-ray diffraction (XRD) and the high-resolution transmission electron microscopy (HRTEM) results revealed the face-centered cubic structure of AgNPs had a highly crystalline with the particle size in a range of 10−20 nm. The Fourier transform infrared spectroscopy (FT-IR) demonstrated the presence of flavonoid, terpenoid, phenolic, and glycosides in phytochemical compositions of CMP extract which can act as the reducing agents for AgNPs formation. The antibacterial effect of the AgNPs was evaluated against Methicillin-resistant Staphylococcus aureus (MRSA) by implementing the minimum inhibitory concentration (MIC), minimum batericidal concentration (MBC), and the zone of inhibition tests. The AgNPs exhibited effective antibacterial activity against bacteria with an average diameter of inhibition zones of 11.7 mm, the MIC of 8.27 µg/mL, and the MBC of the 16.54 µg/mL.

scholarly journals EVALUATION OF ANTIBACTERIAL ACTIVITY OF SILVER NANOPARTICLES AGAINST METHICILLIN-RESISTANT STAPHYLOCOCCUS AUREUS AND DETECTION OF VIRULENCE FACTORS - NUCLEASE, PHOSPHATASE, AND BIO FILM PRODUCTION

2018 ◽  
Vol 11 (5) ◽  
pp. 224 ◽  
Author(s):  
Manipriya B ◽  
Tasneem Banu ◽  
Prem Kumar L ◽  
Kalyani M
Keyword(s):  
Staphylococcus Aureus ◽  
Silver Nanoparticles ◽  
Antibacterial Activity ◽  
Virulence Factors ◽  
Bactericidal Activity ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Nano Particles ◽  
Clinical Isolates ◽  
Ag Nps ◽  
Methicillin Resistant

 Objective: To determine the virulence factors-biofilm, nuclease and phosphatase production in Staphylococcus aureus isolates. To determine the effect of silver nano particles and antibiotics on MRSA by MIC determination and kirby baeur method respectively and finally to compare antibacterial activity of silver nano particles and antibiotics.Methods: In the present study, we explore the antibacterial activity of silver nanoparticles (Ag-NPs) dispersion (10 nm) against reference strain and clinical isolates of methicillin-resistant Staphylococcus aureus (MRSA). We evaluated the antibacterial activity of Ag-NPs against MRSA isolated from patients in Saveetha Medical College and Hospital, a tertiary care centre in Chennai, Tamil Nadu. The bactericidal activity of different concentrations of Ag-NPs (200, 100, 50, 25, 12.5, 6.25, 3.125, and 1.5625 μg/ml) was tested by determining MIC using microbroth dilution and MBC by agar dilution methods.. In addition, the virulence factors phosphatase, nuclease, and biofilm production were tested.Result: The values of minimal inhibitory concentration and minimal bactericidal concentration of Ag-NPs against all clinical isolates of MRSA and a single of S. aureus were found in the range of 12.5–50 μg/ml and 12.5–25 μg/ml, respectively, indicating very good bactericidal activity. Ag-NPs with the highest concentration showed almost no growth for up to 16 h representing a bactericidal effect at this concentration. Effect was proportional to dose since 50.0 μg/ml was the most effective dose since the bacterial population did not recover and 12.5 μg/ml was the least effective. All the MRSA isolates were positive for the virulence factors.Conclusion: The study result suggests that Ag-NPs could be used as an effective alternative antibacterial agent.

Antibacterial Activity of Ecklonia cava Against Methicillin-Resistant Staphylococcus aureus and Salmonella spp.

2010 ◽  
Vol 7 (4) ◽  
pp. 435-441 ◽  
Author(s):  
Jang-Gi Choi ◽  
Ok-Hwa Kang ◽  
Obiang-Obounou Brice ◽  
Young-Seob Lee ◽  
Hee-Sung Chae ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Ecklonia Cava ◽  
Salmonella Spp ◽  
Methicillin Resistant
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