Multiple surface interaction mechanisms direct the anchoring, co-aggregation and formation of dual-species biofilm between Candida albicans and Helicobacter pylori

Yeasts can adapt to a wide range of pH fluctuations (2 to 10), while Helicobacter pylori, a facultative intracellular bacterium, can adapt to a range from pH 6 to 8. This work analyzed if H. pylori J99 can protect itself from acidic pH by entering into Candida albicans ATCC 90028. Growth curves were determined for H. pylori and C. albicans at pH 3, 4, and 7. Both microorganisms were co-incubated at the same pH values, and the presence of intra-yeast bacteria was evaluated. Intra-yeast bacteria-like bodies were detected using wet mounting, and intra-yeast binding of anti-H. pylori antibodies was detected using immunofluorescence. The presence of the H. pylori rDNA 16S gene in total DNA from yeasts was demonstrated after PCR amplification. H. pylori showed larger death percentages at pH 3 and 4 than at pH 7. On the contrary, the viability of the yeast was not affected by any of the pHs evaluated. H. pylori entered into C. albicans at all the pH values assayed but to a greater extent at unfavorable pH values (pH 3 or 4, p = 0.014 and p = 0.001, respectively). In conclusion, it is possible to suggest that H. pylori can shelter itself within C. albicans under unfavorable pH conditions.

Download Full-text

The identification of surface interaction of apotransferrin with Candida albicans

Archives of Pharmacal Research ◽

10.1007/s12272-013-0301-5 ◽

2013 ◽

Vol 37 (10) ◽

pp. 1301-1307 ◽

Cited By ~ 1

Author(s):

Yongmoon Han

Keyword(s):

Candida Albicans ◽

Surface Interaction

Download Full-text

Mikroorganismoek minbizia eragin dezakete?

EKAIA Euskal Herriko Unibertsitateko Zientzi eta Teknologi Aldizkaria ◽

10.1387/ekaia.19667 ◽

2018 ◽

pp. 9-28

Author(s):

Aitana Arbizu ◽

Aitziber Antoran ◽

Idoia Buldain ◽

Aize Pellon ◽

Xabier Guruceaga ◽

...

Keyword(s):

Helicobacter Pylori ◽

Candida Albicans ◽

Chlamydia Pneumoniae ◽

Salmonella Typhi ◽

Streptococcus Bovis

Ikerketa askok mikroorganismoen eta minbizien arteko erlazioak aztertu dituzte, eta erakutsi dute mikroorganismo batzuek minbiziaren agerpena saihesten dutela eta beste batzuek, aldiz, minbizia eragin dezaketela. Hain zuzen ere, gero eta artikulu zientifiko gehiago argitaratzen ari dira mikroorganismoak minbiziaren sortzearekin, ezarpenarekin eta sakabanaketarekin erlazionatuz. Izan ere, mikroorganismoek minbizi guztien % 17,8 eragiten dutela estimatu da. Minbizia sortzeko birusen gaitasuna da gehien ikertu dena eta, ondorioz, minbizia sor dezaketen mekanismo desberdin asko deskribatu dira. Minbizia Ikertzeko Nazioarteko Agentziak zortzi birus 1. mailako «gizakiontzat kartzinogeno»-tzat sailkatu ditu; haien artean, giza papiloma birusa, bi herpesbirus eta bi hepatitisaren birus aurkitzen dira. Bakterioei dagokienez, minbizi-eragileen artean, Helicobacter pylori da gehien ikertu dena urdaileko minbiziarekin erlazionatuta. Baina honetaz gain, beste hainbat bakterio, hala nola Salmonella typhi, Chlamydia pneumoniae eta Streptococcus bovis minbiziarekin zuzenki erlazionatu dira. Onddoek daukaten minbiziarekiko erlazioa oso gutxi ikertu den arren, mikroorganismo hauek sortutako toxina batzuek minbizia eragin dezaketela frogatu da. Horrez gain, Candida albicans onddoak minbiziaren sorrera eta hedapena eragin dezakeen hainbat mekanismo deskribatu dira. Orain arte egindako ikerketek mikroorganismoek minbiziaren garapenean eta sustapenean daukaten eragina agerrarazi dute. Hori dela eta, etorkizunean minbiziari aurre egiteko, minbiziaren eta mikroorganismoen arteko erlazioan sakontzea ezinbestekoa da.

Download Full-text

In vitro antimicrobial activity of hydrosol from Litsea cubeba (Lour.) Pers. against Helicobacter pylori and Candida albicans

Biomedical Research and Therapy ◽

10.15419/bmrat.v7i6.610 ◽

2020 ◽

Vol 7 (6) ◽

pp. 3819-3828

Author(s):

Tran Thanh Hung ◽

Pham Thu Trang ◽

Hoang Viet ◽

Nguyen Thi My Lan ◽

Luong Thi My Ngan ◽

...

Keyword(s):

Helicobacter Pylori ◽

Candida Albicans ◽

Steam Distillation ◽

Antimicrobial Agents ◽

Gas Chromatography Mass Spectrometry ◽

Minimal Bactericidal Concentration ◽

Litsea Cubeba ◽

H Pylori ◽

Antibacterial And Antifungal

Introduction: Helicobacter pylori and Candida albicans are classified as the most common pathogenic agents in humans. H. pylori is responsible for gastroduodenal diseases and greatly associated with gastric carcinogenesis, while C. albicans is the main cause of fungal urinary tract, genital yeast, and fungal skin infections. The increasing appearance of drug-resistant strains of H. pylori and C. albicans has made the treatment of the infections more serious. Hydrosols from plant steam distillation have been traditionally used in medicine, cosmetics, and culinary uses. They have been recently suggested as antimicrobial agents owing to their safety and ability to reduce the potential of resistance. The aim of the present study is to assess antibacterial and antifungal activities of hydrosols extracted from the fresh fruits of Litsea cubeba against H. pylori and C. albicans. Methods: The L. cubeba fruit hydrosol was obtained by steam distillation method. Evaluation of the growth-inhibiting and microbicidal effects of the hydrosol towards the H. pylori ATCC 43504 and C. albicans ATCC 10231 was determined through MIC (minimal inhibitory concentration), MBC (minimal bactericidal concentration), and MFC (minimal fungicidal concentration) measurements using broth dilution assays. Compositions of the dissolved essential oil (dEO) from the hydrosol were analyzed by GC-MS (gas chromatography-mass spectrometry). Results: The results indicated that the L. cubeba fruit hydrosol exhibited strong antimicrobial ability towards the bacterium H. pylori (MIC of 10%, MBC of 30%) and the yeast C. albicans (MIC of 10%, MFC of 40%). The cells of H. pylori and C. albicans were killed completely after 24 and 18 hours of treatment with 30% and 40% of the hydrosol, respectively. The major constituents of the dEO were geranial (32.92%), neral (27.12%), p-menthan-8-yl acetate (8.45%), 2-cyclopropyl-2-methylspiro[2.2]pentane-1-carboxylic acid (8.09%), linalool (4.24%), and methyl heptenone (4.15%). Conclusion: The results of the study suggest that L. cubeba fruit hydrosols could be used as potent natural antibacterial and antifungal preparations in the global effort to discover safe alternatives to toxic antimicrobial agents.

Download Full-text