A rapid method for simultaneous evaluation of free light chain content and aggregate content in culture media of Chinese hamster ovary cells expressing monoclonal antibodies for cell line screening

2016 ◽  
Vol 121 (4) ◽  
pp. 464-470 ◽  
Author(s):  
Yoichi Ishii ◽  
Masayoshi Tsukahara ◽  
Kaori Wakamatsu
Keyword(s):  
Monoclonal Antibodies ◽  
Cell Line ◽  
Light Chain ◽  
Chinese Hamster Ovary ◽  
Culture Media ◽  
Chinese Hamster Ovary Cells ◽  
Chinese Hamster ◽  
Ovary Cells ◽  
Aggregate Content ◽  
Simultaneous Evaluation

Predicting the expression of recombinant monoclonal antibodies in Chinese hamster ovary cells based on sequence features of the CDR3 domain

2013 ◽  
Vol 30 (1) ◽  
pp. 188-197 ◽  
Author(s):  
Leon P. Pybus ◽  
David C. James ◽  
Greg Dean ◽  
Tim Slidel ◽  
Colin Hardman ◽  
...  
Keyword(s):  
Monoclonal Antibodies ◽  
Chinese Hamster Ovary ◽  
Chinese Hamster Ovary Cells ◽  
Chinese Hamster ◽  
Ovary Cells ◽  
Sequence Features

scholarly journals Enhancement of cytotoxicities of ricin and Pseudomonas toxin in Chinese hamster ovary cells by nigericin.

1981 ◽  
Vol 1 (6) ◽  
pp. 552-559 ◽  
Author(s):  
B Ray ◽  
H C Wu
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Protein Synthesis ◽  
Cell Line ◽  
Chinese Hamster Ovary ◽  
Cho Cells ◽  
Diphtheria Toxin ◽  
Chinese Hamster Ovary Cells ◽  
Chinese Hamster ◽  
Ovary Cells ◽  
Pseudomonas Aeruginosa Exotoxin

Nigericin and monensin, ionophores for Na+ and K+, have been found to enhance the cytotoxicities of abrin, ricin, and Pseudomonas aeruginosa exotoxin A in Chinese hamster ovary (CHO) cells. They do not affect the cytotoxicity of diphtheria toxin in the same cell line. Maximal sensitization of the CHO cells toward ricin and Pseudomonas toxin requires preculture of CHO cells in the presence of nigericin. Inhibition of protein synthesis in CHO cells by ricin or Pseudomonas toxin is also enhanced by preculture of CHO cells in the presence of nigericin. These results suggest a common step in the intoxication process of ricin and Pseudomonas toxin, the rate of which is facilitated by pretreatment with nigericin. This step is, however, not shared by the intoxication of CHO cells with diphtheria toxin.

scholarly journals PDADMAC flocculation of Chinese hamster ovary cells: Enabling a centrifuge-less harvest process for monoclonal antibodies

mAbs ◽  
2015 ◽  
Vol 7 (2) ◽  
pp. 413-427 ◽  
Author(s):  
Thomas McNerney ◽  
Anne Thomas ◽  
Anna Senczuk ◽  
Krista Petty ◽  
Xiaoyang Zhao ◽  
...  
Keyword(s):  
Monoclonal Antibodies ◽  
Chinese Hamster Ovary ◽  
Chinese Hamster Ovary Cells ◽  
Chinese Hamster ◽  
Ovary Cells
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