Deletion of Aspergillus nidulans cpsA/rseA induces increased extracellular hydrolase production in solid-state culture partly through the high osmolarity glycerol pathway

In the present study, we aimed to obtain a high yield and productivity for glucosamine using a low-cost solid-state culture with Aspergillus sydowii BCRC 31742. The fermentation conditions, such as inoculum biomass, moisture content, and supplemental volume and mineral salt, were chosen to achieve high productivity of glucosamine (GlcN). When the initial supplemental volume used was 3 mL/g substrate, the yield and productivity of GlcN were 48.7 mg/gds and 0.69 mg/gds·h, respectively. This result will be helpful for the industrialization of the process.

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Ellagic Acid Recovery by Solid State Fermentation of Pomegranate Wastes by Aspergillus niger and Saccharomyces cerevisiae: A Comparison

Molecules ◽

10.3390/molecules24203689 ◽

2019 ◽

Vol 24 (20) ◽

pp. 3689 ◽

Cited By ~ 5

Author(s):

Federica Moccia ◽

Adriana C. Flores-Gallegos ◽

Mónica L. Chávez-González ◽

Leonardo Sepúlveda ◽

Stefania Marzorati ◽

...

Keyword(s):

Saccharomyces Cerevisiae ◽

Solid State ◽

Aspergillus Niger ◽

Ellagic Acid ◽

Fold Increase ◽

Industrial Applications ◽

Solvent Ratio ◽

State Culture ◽

Acid Recovery ◽

Solid State Culture

Fermentation in solid state culture (SSC) has been the focus of increasing interest because of its potential for industrial applications. In previous studies SSC of pomegranate wastes by Aspergillus niger has been extensively developed and optimized for the recovery of ellagic acid (EA), a high value bioactive. In this study we comparatively investigated the SSC of powdered pomegranate husks by A. niger and Saccharomyces cerevisiae and evaluated the recovery yields of EA by an ultrasound and microwave-assisted 7:3 water/ethanol extraction. Surprisingly enough, the yields obtained by S. cerevisiae fermentation (4% w/w) were found 5-fold higher than those of the A. niger fermented material, with a 10-fold increase with respect to the unfermented material. The EA origin was traced by HPLC analysis that showed a significant decrease in the levels of punicalagin isomers and granatin B and formation of punicalin following fermentation. Other extraction conditions that could warrant a complete solubilization of EA were evaluated. Using a 1:100 solid to solvent ratio and DMSO as the solvent, EA was obtained in 4% yields from S. cerevisiae fermented husks at a high purity degree. Hydrolytic treatment of S. cerevisiae fermented pomegranate husks afforded a material freed of the polysaccharides components that gave recovery yields of EA up to 12% w/w.

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Optimization of Solid State Culture Conditions for the Production of Adenosine, Cordycepin, and D-mannitol in Fruiting Bodies of Medicinal Caterpillar Fungus Cordyceps militaris (L.:Fr.) Link (Ascomycetes)

International Journal of Medicinal Mushrooms ◽

10.1615/intjmedmushr.v14.i2.60 ◽

2012 ◽

Vol 14 (2) ◽

pp. 181-187 ◽

Cited By ~ 16

Author(s):

LekTeng Lim ◽

ChiaYen Lee ◽

EngThuan Chang

Keyword(s):

Solid State ◽

Culture Conditions ◽

Cordyceps Militaris ◽

Fruiting Bodies ◽

State Culture ◽

Solid State Culture ◽

Caterpillar Fungus

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Yeast osmosensor Sln1 and plant cytokinin receptor Cre1 respond to changes in turgor pressure

The Journal of Cell Biology ◽

10.1083/jcb.200301099 ◽

2003 ◽

Vol 161 (6) ◽

pp. 1035-1040 ◽

Cited By ~ 145

Author(s):

Vladimír Reiser ◽

Desmond C. Raitt ◽

Haruo Saito

Keyword(s):

Cell Wall ◽

Stress Responses ◽

Histidine Kinase ◽

Turgor Pressure ◽

Hyperosmotic Stress ◽

Cytokinin Receptor ◽

High Osmolarity ◽

High Osmolarity Glycerol ◽

High Osmolarity Glycerol Pathway ◽

Cytokinin Response

Very little is known about how cellular osmosensors monitor changes in osmolarity of the environment. Here, we report that in yeast, Sln1 osmosensor histidine kinase monitors changes in turgor pressures. Reductions in turgor caused by either hyperosmotic stress, nystatin, or removal of cell wall activate MAPK Hog1 specifically through the SLN1 branch, but not through the SHO1 branch of the high osmolarity glycerol pathway. The integrity of the periplasmic region of Sln1 was essential for its sensor function. We found that activity of the plant histidine kinase cytokinin response 1 (Cre1) is also regulated by changes in turgor pressure, in a manner identical to that of Sln1, in the presence of cytokinin. We propose that Sln1 and Cre1 are turgor sensors, and that similar turgor-sensing mechanisms might regulate hyperosmotic stress responses both in yeast and plants.

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