scholarly journals Kinetic Study of Glucosamine Production Using Aspergillus sydowii BCRC 31742 under Solid-State Fermentation

Molecules ◽  
2020 ◽  
Vol 25 (20) ◽  
pp. 4832
Author(s):  
Jia Wei Peng ◽  
Ho Shing Wu
Keyword(s):  
Solid State ◽  
Moisture Content ◽  
Solid State Fermentation ◽  
Low Cost ◽  
High Yield ◽  
Aspergillus Sydowii ◽  
Fermentation Conditions ◽  
High Productivity ◽  
State Culture ◽  
Solid State Culture

In the present study, we aimed to obtain a high yield and productivity for glucosamine using a low-cost solid-state culture with Aspergillus sydowii BCRC 31742. The fermentation conditions, such as inoculum biomass, moisture content, and supplemental volume and mineral salt, were chosen to achieve high productivity of glucosamine (GlcN). When the initial supplemental volume used was 3 mL/g substrate, the yield and productivity of GlcN were 48.7 mg/gds and 0.69 mg/gds·h, respectively. This result will be helpful for the industrialization of the process.

scholarly journals Optimization of Lipase Production in Solid-State Fermentation by Rhizopus Arrhizus in Nutrient Medium Containing Agroindustrial Wastes

2018 ◽  
Vol 12 (1) ◽  
pp. 189-203 ◽  
Author(s):  
Georgi Dobrev ◽  
Hristina Strinska ◽  
Anelia Hambarliiska ◽  
Boriana Zhekova ◽  
Valentina Dobreva
Keyword(s):  
Solid State ◽  
Moisture Content ◽  
Solid State Fermentation ◽  
Lipase Activity ◽  
Nutrient Medium ◽  
Solid Medium ◽  
Low Cost ◽  
Lipase Production ◽  
Rhizopus Arrhizus ◽  
Agroindustrial Wastes

Background: Rhizopus arrhizus is a potential microorganism for lipase production. Solid-state fermentation is used for microbial biosynthesis of enzymes, due to advantages, such as high productivity, utilization of abundant and low-cost raw materials, and production of enzymes with different catalytic properties. Objective: The objective of the research is optimization of the conditions for lipase production in solid-state fermentation by Rhizopus arrhizus in a nutrient medium, containing agroindustrial wastes. Method: Biosynthesis of lipase in solid-state fermentation by Rhizopus arrhizus was investigated. The effect of different solid substrates, additional carbon and nitrogen source, particles size and moisture content of the medium on enzyme production was studied. Response surface methodology was applied for determination of the optimal values of moisture content and tryptone concentration. A procedure for efficient lipase extraction from the fermented solids was developed. Results: Highest lipase activity was achieved when wheat bran was used as a solid substrate. The addition of 1% (w/w) glucose and 5% (w/w) tryptone to the solid medium significantly increased lipase activity. The structure of the solid medium including particles size and moisture content significantly influenced lipase production. A mathematical model for the effect of moisture content and tryptone concentration on lipase activity was developed. Highest enzyme activity was achieved at 66% moisture and 5% (w/w) tryptone. The addition of the non-ionic surfactant Disponyl NP 3070 in the eluent for enzyme extraction from the fermented solids increased lipase activity about three folds. Conclusion: After optimization of the solid-state fermentation the achieved 1021.80 U/g lipase activity from Rhizopus arrhizus was higher and comparable with the activity of lipases, produced by other fungal strains. The optimization of the conditions and the use of low cost components in solid-state fermentation makes the process economicaly effective for production of lipase from the investigated strain Rhizopus arrhizus.

Construction of the Starter to Produce Lignocellulases via Solid State Fermentation of Rice Straw

2011 ◽  
Vol 356-360 ◽  
pp. 1819-1822
Author(s):  
Shan Shan Du ◽  
Lu Yang ◽  
Jian Zhang Lu ◽  
Qing Qing Li ◽  
Juan Xu ◽  
...  
Keyword(s):  
Solid State ◽  
Aspergillus Niger ◽  
Moisture Content ◽  
Rice Straw ◽  
Solid State Fermentation ◽  
Low Cost ◽  
Natural Microflora ◽  
Special Strain

The study demonstrates the starter construction and its solid state fermentation (SSF) using rice straw as substrate for lignocellulases production. The starter is constructed by natural microflora growing on rice straw additionally reinforced with Aspergillus niger ZJU-RYD1, using the SSF substrate rice straw with 90% moisture content, successfully produced the integrate and high active lignocellulases at about 72 h, i.e. CMCase 12.44 U/g, cellobiase 12.05 U/g, FPAase 3.18 U/g, xylanase 365.61 U/g, lassase 2.48 U/g. In this study the strategy is satisfactorily developed to construct the starter for high effective and low-cost production of lignocellulases using rice straw as substrate, based on natural microflora and additional reinforcing with special strain.

scholarly journals EFFECT OF FERMENTATION TIME, MOISTURE CONTENT, AND TEMPERATURE ON SORBITOL PRODUCTION VIA SOLID STATE FERMENTATION PROCESS

2017 ◽  
Vol 1 (1) ◽  
pp. 64-71
Author(s):  
Zuriana Sidi Ahmad ◽  
Mimi Sakinah Abdul Munaim
Keyword(s):  
Solid State ◽  
Moisture Content ◽  
Solid State Fermentation ◽  
Incubation Temperature ◽  
Product Yield ◽  
High Yield ◽  
Time Range ◽  
Fermentation Time ◽  
Wood Sawdust ◽  
Ssf Process

Malaysia is the largest country that has produced many types of waste. One of it is Meranti wood sawdust. These wastes result in a significant environmental problem if not dispose it in the proper manner. The main objective of this article is to produce the high yield of sorbitol by solid state fermentation (SSF) process from pretreated Meranti wood sawdust using bacterium Lactobacillus plantarum (BAA 793; NCIMB 8826). One factorat a time (OFAT) was studied for further process using solid state fermentation (SSF) process and investigated the effect of relevant parameters (fermentation time, range: 2 hours to 14 hours, moisture content, range: 40% to 90%, temperature, range: 25 oC to 45 oC) to the solid-state fermentation (SSF) process in producing high yield of sorbitol. The highest product yield was obtained at 50% moisture content, at 10 hours of fermentation time and 35 oC of incubation temperature where the concentration of sorbitol was 25.68 g/L respectively. This study also showed that the solid state fermentation (SSF) process will produce the high yield of sorbitol production compared to the submerged fermentation and could serve as a-low cost substrate for bioproducts production especially sorbitol

Pectinase Production from Banana Peel Biomass via the Optimization of the Solid-state Fermentation Conditions of Aspergillus niger Strain

2021 ◽  
Vol 30 (1) ◽  
pp. 257-275
Author(s):  
Nazaitulshila Rasit ◽  
Yong Sin Sze ◽  
Mohd Ali Hassan ◽  
Ooi Chee Kuan ◽  
Sofiah Hamzah ◽  
...  
Keyword(s):  
Solid State ◽  
Moisture Content ◽  
Solid State Fermentation ◽  
Incubation Temperature ◽  
Inoculum Size ◽  
Operating Conditions ◽  
Banana Peel ◽  
Fermentation Conditions ◽  
Pectinase Production ◽  
Full Factorial

In this study, the biomass of banana peel was used to produce pectinase via optimization of solid-state fermentation conditions of the filamentous fungi Aspergillus nigeA. niger). The operating conditions of solid-state fermentation were optimized using the method of full factorial design with incubation temperature ranging between 25 °C and 35 °C, moisture content between 40% and 60%, and inoculum size between 1.6 x 106 spores/mL and 1.4 x 107 spores/mL. Optimizing the solid-state fermentation conditions appeared crucial to minimize the sample used in this experimental design and determine the significant correlation between the operating conditions. A relatively high maximal pectinase production of 27 UmL-1 was attained at 35° C of incubation, 60% of moisture content, and 1.6 x 106 spores/mL of inoculum size with a relatively low amount of substrate (5 g). Given that the production of pectinase with other substrates (e.g., pineapple waste, lemon peel, cassava waste, and wheat bran) generally ranges between 3 U/mL and 16 U/mL (Abdullah et al., 2018; Handa et al., 2016; Melnichuk et al., 2020; Thangaratham and Manimegalai, 2014; Salim et al., 2017), thus the yield of pectinase derived from the banana peel in this study (27 U/mL) was considered moderately high. The findings of this study indicated that the biomass of banana peel would be a potential substrate for pectinase production via the solid-state fermentation of A. niger.

scholarly journals Effects of nutriental and environmental conditions on carotenoid biosynthesis by Rhodotorula sp.

2020 ◽  
Vol 8 (2) ◽  
pp. 22-29
Author(s):  
Ly Thi Minh Hien ◽  
Dong Thi Anh Dao
Keyword(s):  
Solid State ◽  
Environmental Factors ◽  
Moisture Content ◽  
Solid State Fermentation ◽  
Environmental Conditions ◽  
Low Cost ◽  
Carotenoid Biosynthesis ◽  
Natural Antioxidants ◽  
Fermentation Time ◽  
Agricultural Byproduct

Carotenoid compounds are popular natural antioxidants which are commonly isolated from the plants. Recently, there have been many researches on carotenoid biosynthesis towards low cost products. In this study, Rhodotorula sp. was grown on an agricultural byproduct (corncobs) as a matrix in solid-state fermentation. Essential nutrients were added with different concentrations to optimize condition for the carotenoid biosynthesis. Effects of other environmental factors such as moisture content and fermentation time on the yield were also characterized. The optimal nutrient composition for the yeast’s growth and carotenoid biosynthesis is a compound of 500μg nitrogen and 16mg carbon in 100g matrix. Additionally, the moisture content of 80% is the best for producing carotenoid by this yeast strain. The fermentation time for the highest carotenoid yield is observed after 8 days.

scholarly journals An overview of the solid state fermentation in the production of fungal protease enzymes

2021 ◽  
Vol 9 (3) ◽  
pp. 085-089
Author(s):  
Sambo S ◽  
Magashi AM ◽  
Farouq AA ◽  
Hassan SW
Keyword(s):  
Solid State ◽  
Solid State Fermentation ◽  
Low Cost ◽  
Industrial Processes ◽  
High Productivity ◽  
Human Needs ◽  
Fermented Product ◽  
Metabolites Production ◽  
Fungal Protease ◽  
Enzyme Source

Enzymes are among the most important products obtained for human needs through microbial sources. A large number of industrial processes in the area of industrial, environmental and food technology utilize enzymes at some stage or another; Solid State Fermentation (SSF) holds tremendous potential for the production of enzymes, especially in those processes where the crude fermented product may be used directly as the enzyme source. Fungal proteases are used in many industrial processes for the production of foods and metabolites, production of enzymes from fungi offered many advantages which include low cost and high productivity. Hence because of the higher yielding capacity of SSF and the demand for proteases it highly imperative to search for novel microorganisms from possible environment and subject them to SSF for protease investigation to add up to the nation need of the enzymes and boast economy.

Construction of the Starter to Produce Lignocellulases via Solid State Fermentation of Metropolis Lignocellulosic Waste Defoliation

2011 ◽  
Vol 356-360 ◽  
pp. 1823-1826
Author(s):  
Lu Yang ◽  
Shan Shan Du ◽  
Jian Zhang Lu ◽  
Qing Qing Li ◽  
Juan Xu ◽  
...  
Keyword(s):  
Solid State ◽  
Aspergillus Niger ◽  
Moisture Content ◽  
Wheat Bran ◽  
Solid State Fermentation ◽  
Low Cost ◽  
Lignocellulosic Waste ◽  
Natural Microflora ◽  
Special Strain

The study demonstrates the starter construction and its solid state fermentation (SSF) using metropolis lignocellulosic waste defoliation supplemented with wheat bran as substrates for lignocellulases production. The starter is constructed by natural microflora growing on defoliation additionally reinforced with Aspergillus niger ZJU-RYD1, using the SSF substrate defoliation with 90% moisture content, successfully produced the integrate and high active lignocellulases at about 120 h, i.e. CMCase 8.58 U/g, cellobiase 10.97 U/g, FPAase 2.21 U/g, xylanase 446.25 U/g, lassase 9.21U/g. In this study, the strategy is satisfactorily developed to construct the starter for high effective and low-cost production of lignocellulases using metropolis lignocellulosic waste defoliation as substrate, based on natural microflora and additional reinforcing with special strain

scholarly journals Spent Coffee Waste as a Potential Media Component for Xylanase Production and Potential Application in Juice Enrichment

Foods ◽  
2019 ◽  
Vol 8 (11) ◽  
pp. 585 ◽  
Author(s):  
Rajeev Ravindran ◽  
Gwilym A. Williams ◽  
Amit K. Jaiswal
Keyword(s):  
Solid State ◽  
Moisture Content ◽  
Solid State Fermentation ◽  
Initial Moisture Content ◽  
Medium Composition ◽  
Xylanase Production ◽  
Box Behnken Design ◽  
Fermentation Conditions ◽  
Coffee Waste ◽  
Media Component

In this study, spent coffee waste (SCW) was used as the sole carbon source for xylanase production in solid state fermentation mode using Aspergillus niger. A Box–Behnken design was constructed using three parameters viz. temperature, initial moisture content, and log number of spores to determine the optimal fermentation condition. The best fermentation conditions for xylanase production were found to be incubation at 30 °C with an initial moisture content of 70% and using an inoculum of 6.5 × 106 spores/g of dry SCW. Furthermore, the design of experiments revealed that maintaining a medium composition of 0.2 g of yeast extract, 0.04 g of K2HPO4, and 0.03 g of MgSO4 increased xylanase production. Under optimised solid-state fermentation conditions an enzyme activity of 6495.6 IU/g of dry SCW was recorded, which was approximately 1.39-fold higher than that of control (4649 IU/g of dry SCW). The efficacy of the purified xylanase as a juice enrichment agent for strawberry, blueberry, and raspberry pulp was tested.

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