Conservation of in vitro shoots of Rhodiola sachalinensis by slow growth and genetic stability of recovered plantlets

2008 ◽  
Vol 136 ◽  
pp. S154
Author(s):  
Liu Jian-feng ◽  
Cheng Yun-qing ◽  
Yang Jun
Keyword(s):  
Genetic Stability ◽  
Slow Growth ◽  
Rhodiola Sachalinensis ◽  
In Vitro Shoots

STUDY OF SLOW GROWTH CONDITIONS OF RANUNCULUS IN VITRO SHOOTS

2011 ◽  
pp. 391-403 ◽  
Author(s):  
M. Beruto ◽  
S. Rinino ◽  
A. Bisignano ◽  
M. Fibiani
Keyword(s):  
Slow Growth ◽  
Growth Conditions ◽  
In Vitro Shoots

Isozyme assessment of the genetic stability of micropropagated hybrid larch (Larix × eurolepis Henry)

1995 ◽  
Vol 25 (7) ◽  
pp. 1103-1112 ◽  
Author(s):  
Sylvie Richard ◽  
Sylvie Gauthier ◽  
Sylvie Laliberté
Keyword(s):  
Tissue Culture ◽  
Growth Regulators ◽  
Genetic Stability ◽  
Physiological Response ◽  
Culture Conditions ◽  
Hybrid Larch ◽  
Short Shoot ◽  
In Vitro Shoots ◽  
The Media

The search for the occurrence of somaclonal variation of in vitro shoots and acclimatized plants of a hybrid larch (Larix × urolepis Henry) clone was performed by the analysis of eight isozyme systems. Cultures were established from short shoot buds of mature material. The effects of growth regulators in the media, subculture intervals, and periods in culture were analyzed for in vitro shoots. Variability was found in in vitro shoots but appeared to be related to a physiological response to culture conditions. Once acclimatized, most tissuecultured plants expressed the same enzymatic patterns as those of control plants (stecklings and the ortet). The variations observed for some acclimatized plants were also observed in control plants and were not related to ontogenic stage. Results from the isoenzymatic systems studied showed that hybrid larch plants regenerated from tissue culture were not significantly different from stecklings and the ortet.

scholarly journals Phytotoxicity and Other Adverse Effects on the In Vitro Shoot Cultures Caused by Virus Elimination Treatments: Reasons and Solutions

Plants ◽  
2021 ◽  
Vol 10 (4) ◽  
pp. 670
Author(s):  
Katalin Magyar-Tábori ◽  
Nóra Mendler-Drienyovszki ◽  
Alexandra Hanász ◽  
László Zsombik ◽  
Judit Dobránszki
Keyword(s):  
Adverse Effects ◽  
Physiological Condition ◽  
Harmful Effect ◽  
Shoot Cultures ◽  
Virus Elimination ◽  
In Vitro Shoots ◽  
Further Development ◽  
In Vitro Shoot Cultures ◽  
Harmful Effects

In general, in vitro virus elimination is based on the culture of isolated meristem, and in addition thermotherapy, chemotherapy, electrotherapy, and cryotherapy can also be applied. During these processes, plantlets suffer several stresses, which can result in low rate of survival, inhibited growth, incomplete development, or abnormal morphology. Even though the in vitro cultures survive the treatment, further development can be inhibited; thus, regeneration capacity of treated in vitro shoots or explants play also an important role in successful virus elimination. Sensitivity of genotypes to treatments is very different, and the rate of destruction largely depends on the physiological condition of plants as well. Exposure time of treatments affects the rate of damage in almost every therapy. Other factors such as temperature, illumination (thermotherapy), type and concentration of applied chemicals (chemo- and cryotherapy), and electric current intensity (electrotherapy) also may have a great impact on the rate of damage. However, there are several ways to decrease the harmful effect of treatments. This review summarizes the harmful effects of virus elimination treatments applied on tissue cultures reported in the literature. The aim of this review is to expound the solutions that can be used to mitigate phytotoxic and other adverse effects in practice.

scholarly journals Germplasm Conservation: Instrumental in Agricultural Biodiversity—A Review

2021 ◽  
Vol 13 (12) ◽  
pp. 6743
Author(s):  
Veerala Priyanka ◽  
Rahul Kumar ◽  
Inderpreet Dhaliwal ◽  
Prashant Kaushik
Keyword(s):  
Plant Species ◽  
Agricultural Production ◽  
Genetic Instability ◽  
Slow Growth ◽  
Germplasm Conservation ◽  
Agricultural Biodiversity ◽  
Genetic Composition ◽  
In Vitro Techniques ◽  
Protection Strategies

Germplasm is a valuable natural resource that provides knowledge about the genetic composition of a species and is crucial for conserving plant diversity. Germplasm protection strategies not only involve rescuing plant species threatened with extinction, but also help preserve all essential plants, on which rests the survival of all organisms. The successful use of genetic resources necessitates their diligent collection, storage, analysis, documentation, and exchange. Slow growth cultures, cryopreservation, pollen and DNA banks, botanical gardens, genetic reserves, and farmers’ fields are a few germplasm conservation techniques being employed. However, the adoption of in-vitro techniques with any chance of genetic instability could lead to the destruction of the entire substance, but the improved understanding of basic regeneration biology would, in turn, undoubtedly increase the capacity to regenerate new plants, thus expanding selection possibilities. Germplasm conservation seeks to conserve endangered and vulnerable plant species worldwide for future proliferation and development; it is also the bedrock of agricultural production.

scholarly journals Primula veris plants derived from in vitro cultures and from seeds: genetic stability, morphology, and seed characteristics

2018 ◽  
Vol 42 (4) ◽  
pp. 412-422 ◽  
Author(s):  
Iwona JEDRZEJCZYK ◽  
Maria MOROZOWSKA ◽  
Renata NOWIŃSKA ◽  
Andrzej M. JAGODZIŃSKI
Keyword(s):  
Genetic Stability ◽  
In Vitro Cultures ◽  
Primula Veris ◽  
Seed Characteristics

scholarly journals Chemical and genetic stability of methyl chavicol-rich Indian basil (Ocimum basilicum var. CIM-Saumya) micropropagated in vitro

2017 ◽  
Vol 113 ◽  
pp. 186-191 ◽  
Author(s):  
M. Kumari ◽  
D. Agnihotri ◽  
C.S. Chanotiya ◽  
A.K. Mathur ◽  
R.K. Lal ◽  
...  
Keyword(s):  
Genetic Stability ◽  
Ocimum Basilicum ◽  
Methyl Chavicol

scholarly journals ASSESSMENT OF GENETIC STABILITY OF MICROPROPAGATED Eucalyptus globulus Labill HYBRID CLONES BY MEANS OF FLOW CYTOMETRY AND MICROSATELLITES MARKERS

2017 ◽  
Vol 41 (1) ◽  
Author(s):  
Leandro Silva Oliveira ◽  
Aloisio Xavier ◽  
Wagner Campos Otoni ◽  
José Marcello Salabert Campos ◽  
Lyderson Facio Viccini ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Microsatellite Markers ◽  
Genetic Stability ◽  
Culture Medium ◽  
Eucalyptus Grandis ◽  
Genetic Fidelity ◽  
Shoot Multiplication ◽  
Micropropagated Plants ◽  
Hybrid Clones

ABSTRACT Flow cytometry and microsatellite markers were used to determine a genetic fidelity of micropropagated plants from the two Eucalyptus urophylla x E. globulus clones and a Eucalyptus grandis x E. globulus clone derived from adult material. Clones were repeatedly subcultured for 25 subcultures on MS medium supplemented with BA (2.22 µM) and ANA (0.05 µM) for in vitro shoot multiplication. The elongation was performed in MS culture medium supplemented with AIB (2.46 µM) and BA(0.22 µM). The ex vitro rooting and acclimatization phases were lead at the same time. The micropropagated clones showed genetic stability by flow cytometry and microsatellite markers. The results proved that micropropagation, for purposes of rejuvenation, can be a viable technique to generate genetically stable or identical E. globulus hybrid clones.

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