scholarly journals Isolation of antigen-binding camelid heavy chain antibody fragments (nanobodies) from an immune library displayed on the surface of Pichia pastoris

2010 ◽  
Vol 145 (2) ◽  
pp. 93-98 ◽  
Author(s):  
Stefan Ryckaert ◽  
Els Pardon ◽  
Jan Steyaert ◽  
Nico Callewaert
Keyword(s):  
Pichia Pastoris ◽  
Heavy Chain ◽  
Antibody Fragments ◽  
Antigen Binding ◽  
Heavy Chain Antibody ◽  
Immune Library

Improved production and function of llama heavy chain antibody fragments by molecular evolution

2000 ◽  
Vol 80 (3) ◽  
pp. 261-270 ◽  
Author(s):  
Richard H.J van der Linden ◽  
Bernard de Geus ◽  
Leon G.J Frenken ◽  
Hans Peters ◽  
C.Theo Verrips
Keyword(s):  
Molecular Evolution ◽  
Heavy Chain ◽  
Antibody Fragments ◽  
Heavy Chain Antibody ◽  
And Function

scholarly journals Expression and production of llama variable heavy-chain antibody fragments (VHHs) by Aspergillus awamori

2004 ◽  
Vol 66 (6) ◽  
pp. 737-737 ◽  
Author(s):  
Vivi Joosten ◽  
Robin J. Gouka ◽  
Cees A. M. J. J. van den Hondel ◽  
C. Theo Verrips ◽  
B. Christien Lokman
Keyword(s):  
Heavy Chain ◽  
Antibody Fragments ◽  
Aspergillus Awamori ◽  
Variable Heavy ◽  
Heavy Chain Antibody

Transmigration of beta amyloid specific heavy chain antibody fragments across the in vitro blood–brain barrier

Neuroscience ◽  
2011 ◽  
Vol 190 ◽  
pp. 37-42 ◽  
Author(s):  
K.S. Rutgers ◽  
R.J.A. Nabuurs ◽  
S.A.A. van den Berg ◽  
G.J. Schenk ◽  
M. Rotman ◽  
...  
Keyword(s):  
Blood Brain Barrier ◽  
Heavy Chain ◽  
Beta Amyloid ◽  
Antibody Fragments ◽  
Brain Barrier ◽  
Blood Brain ◽  
Heavy Chain Antibody

scholarly journals In Vivo Detection of Amyloid-β Deposits Using Heavy Chain Antibody Fragments in a Transgenic Mouse Model for Alzheimer's Disease

PLoS ONE ◽  
2012 ◽  
Vol 7 (6) ◽  
pp. e38284 ◽  
Author(s):  
Rob J. A. Nabuurs ◽  
Kim S. Rutgers ◽  
Mick M. Welling ◽  
Athanasios Metaxas ◽  
Maaike E. de Backer ◽  
...  
Keyword(s):  
Alzheimer’S Disease ◽  
Mouse Model ◽  
Transgenic Mouse ◽  
Heavy Chain ◽  
Amyloid Β ◽  
Transgenic Mouse Model ◽  
Antibody Fragments ◽  
In Vivo Detection ◽  
Heavy Chain Antibody

LLAMA HEAVY-CHAIN ANTIBODY FRAGMENTS EFFICIENTLY REMOVE TOXIC SHOCK SYNDROME TOXIN 1 FROM PLASMA IN VITRO BUT NOT IN EXPERIMENTAL PORCINE SEPTIC SHOCK

Shock ◽  
2010 ◽  
Vol 34 (2) ◽  
pp. 125-132 ◽  
Author(s):  
Walter J. Brummelhuis ◽  
Jaap A. Joles ◽  
Jord C. Stam ◽  
Hendrik Adams ◽  
Roel Goldschmeding ◽  
...  
Keyword(s):  
Septic Shock ◽  
Heavy Chain ◽  
Toxic Shock Syndrome ◽  
Antibody Fragments ◽  
Toxic Shock ◽  
Heavy Chain Antibody ◽  
Toxic Shock Syndrome Toxin

scholarly journals Lactobacilli Expressing Variable Domain of Llama Heavy‐Chain Antibody Fragments (Lactobodies) Confer Protection against Rotavirus‐Induced Diarrhea

2006 ◽  
Vol 194 (11) ◽  
pp. 1580-1588 ◽  
Author(s):  
Neha Pant ◽  
Anna Hultberg ◽  
Yaofeng Zhao ◽  
Lennart Svensson ◽  
Qiang Pan‐Hammarström ◽  
...  
Keyword(s):  
Heavy Chain ◽  
Antibody Fragments ◽  
Variable Domain ◽  
Heavy Chain Antibody

Expression and production of llama variable heavy-chain antibody fragments (VHHs) by Aspergillus awamori

2004 ◽  
Vol 66 (4) ◽  
pp. 384-392 ◽  
Author(s):  
Vivi Joosten ◽  
Robin J. Gouka ◽  
Cees A. M. J. J. van den Hondel ◽  
C. Theo Verrips ◽  
B. Christien Lokman
Keyword(s):  
Heavy Chain ◽  
Antibody Fragments ◽  
Aspergillus Awamori ◽  
Variable Heavy ◽  
Heavy Chain Antibody

scholarly journals Generation of Monoclonal Antibodies for Sensitive Detection of Pro-Inflammatory Protein S100A9

2021 ◽  
Vol 11 (10) ◽  
pp. 4659
Author(s):  
Eun-Jung Kim ◽  
Gyu-Min Im ◽  
Chang-Soo Lee ◽  
Yun-Gon Kim ◽  
Byoung Joon Ko ◽  
...  
Keyword(s):  
Monoclonal Antibodies ◽  
Calcium Binding ◽  
Nucleotide Sequences ◽  
Antibody Fragments ◽  
High Yield ◽  
Antigen Binding ◽  
Hybridoma Cell Culture ◽  
Inflammatory Protein ◽  
Inflammatory Processes ◽  
Yield And Purity

The calcium-binding protein S100A9 regulates inflammatory processes and the immune response. It is overexpressed in a variety of inflammatory and oncologic conditions. In this study, we produced a recombinant human S100A9 (hS100A9) antigen with high yield and purity and used it to generate a hybridoma cell culture-based monoclonal anti-hS100A9 antibody. We selected five anti-hS100A9 antibodies from cell supernatants that showed high antigen binding efficiency and identified the nucleotide sequences of three antibodies: two with high effective concentration values and one with the lowest value. The antigen and antibody development procedures described herein are useful for producing large amounts of monoclonal antibodies against hS100A9 and other antigens of interest. The nucleotide sequences of the anti-hS100A9 monoclonal antibody revealed herein will be helpful in the generation of recombinant antibodies or antibody fragments against hS100A9.

Over expression of anti-MUC1 single-domain antibody fragments in the yeast Pichia pastoris

2006 ◽  
Vol 43 (5) ◽  
pp. 426-435 ◽  
Author(s):  
Fatemeh Rahbarizadeh ◽  
Mohammad J. Rasaee ◽  
Mehdi Forouzandeh ◽  
Abdol-Amir Allameh
Keyword(s):  
Pichia Pastoris ◽  
Antibody Fragments ◽  
Single Domain ◽  
Single Domain Antibody ◽  
Yeast Pichia Pastoris ◽  
Over Expression ◽  
Domain Antibody
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