Immobilized lipase-CLEA aggregates encapsulated in lentikats® as robust biocatalysts for continuous processes in deep eutectic solvents

2020 ◽  
Vol 310 ◽  
pp. 97-102 ◽  
Author(s):  
Nadia Guajardo ◽  
Katherine Ahumada ◽  
Pablo Domínguez de María
Keyword(s):  
Immobilized Lipase ◽  
Deep Eutectic Solvents ◽  
Continuous Processes

scholarly journals Development of Effective Lipase-Hybrid Nanoflowers Enriched with Carbon and Magnetic Nanomaterials for Biocatalytic Transformations

Nanomaterials ◽  
2019 ◽  
Vol 9 (6) ◽  
pp. 808 ◽  
Author(s):  
Renia Fotiadou ◽  
Michaela Patila ◽  
Mohamed Amen Hammami ◽  
Apostolos Enotiadis ◽  
Dimitrios Moschovas ◽  
...  
Keyword(s):  
Catalytic Activity ◽  
Immobilized Lipase ◽  
Aqueous Media ◽  
Deep Eutectic Solvents ◽  
Operational Stability ◽  
Magnetic Nanomaterials ◽  
Energy Dispersion Spectroscopy ◽  
Simple Method ◽  
Pseudozyma Antarctica ◽  
Carbon Based Nanomaterials

In the present study, hybrid nanoflowers (HNFs) based on copper (II) or manganese (II) ions were prepared by a simple method and used as nanosupports for the development of effective nanobiocatalysts through the immobilization of lipase B from Pseudozyma antarctica. The hybrid nanobiocatalysts were characterized by various techniques including scanning electron microscopy (SEM), energy dispersion spectroscopy (EDS), X-ray diffraction (XRD), Raman spectroscopy, and Fourier transform infrared spectroscopy (FTIR). The effect of the addition of carbon-based nanomaterials, namely graphene oxide and carbon nanotubes, as well as magnetic nanoparticles such as maghemite, on the structure, catalytic activity, and operational stability of the hybrid nanobiocatalysts was also investigated. In all cases, the addition of nanomaterials during the preparation of HNFs increased the catalytic activity and the operational stability of the immobilized biocatalyst. Lipase-based magnetic nanoflowers were effectively applied for the synthesis of tyrosol esters in non-aqueous media, such as organic solvents, ionic liquids, and environmental friendly deep eutectic solvents. In such media, the immobilized lipase preserved almost 100% of its initial activity after eight successive catalytic cycles, indicating that these hybrid magnetic nanoflowers can be applied for the development of efficient nanobiocatalytic systems.

Highly Efficient Enzymatic Acylation of Dihydromyricetin by the Immobilized Lipase with Deep Eutectic Solvents as Cosolvent

2017 ◽  
Vol 65 (10) ◽  
pp. 2084-2088 ◽  
Author(s):  
Shi-Lin Cao ◽  
Xiao Deng ◽  
Pei Xu ◽  
Zi-Xuan Huang ◽  
Jian Zhou ◽  
...  
Keyword(s):  
Immobilized Lipase ◽  
Deep Eutectic Solvents ◽  
Highly Efficient ◽  
Enzymatic Acylation

Probing the Structural Features and the Micro-heterogeneity of Various Deep Eutectic Solvents and their Water Dilutions by the Photophysical Behaviour of Two Fluorophores

2020 ◽  
Author(s):  
Matteo Tiecco ◽  
Irene Di Guida ◽  
Pier Luigi Gentili ◽  
Raimondo Germani ◽  
Carmela Bonaccorso ◽  
...  
Keyword(s):  
Hydrogen Bond ◽  
Transient Absorption ◽  
Photophysical Properties ◽  
Deep Eutectic Solvents ◽  
Structural Features ◽  
Solvation Dynamics ◽  
Structured Systems ◽  
Reduced Viscosity ◽  
Bond Network ◽  
Fluorescent Molecules

<div><div><div><p>The structural features of a series of diverse Deep Eutectic Solvents (DESs) have been investigated and characterized by means of two fluorescent probes. The spectral and photophysical properties of the latter are strictly dependent on the experienced environment, so that they can provide insights into the polarity, viscosity, hydrogen-bond network, and micro-heterogeneity of the various DESs.</p><p>In fact, the investigated DESs exhibit a variety of properties with regards to their hydrophilicity, acidity, and hydrogen-bond ability, and these details were deeply probed by the two fluorescent molecules. The effect of the addition of water, which is a key strategy for tuning the properties of these structured systems, was also tested. In particular, the excited state dynamics of the probes, measured by femtosecond-resolved transient absorption, proved instrumental in understanding the changes in the structural properties of the DESs, namely reduced viscosity and enhanced heterogeneity, as the water percentage increases. Differences between the various DESs in terms of both local microheterogeneity and bulk viscosity also emerged from the peculiar multi-exponential solvation dynamics undergone by the excited states of the probes.</p></div></div></div>

Probing the Structural Features and the Micro-heterogeneity of Various Deep Eutectic Solvents and their Water Dilutions by the Photophysical Behaviour of Two Fluorophores

2020 ◽  
Author(s):  
Matteo Tiecco ◽  
Irene Di Guida ◽  
Pier Luigi Gentili ◽  
Raimondo Germani ◽  
Carmela Bonaccorso ◽  
...  
Keyword(s):  
Hydrogen Bond ◽  
Transient Absorption ◽  
Photophysical Properties ◽  
Deep Eutectic Solvents ◽  
Structural Features ◽  
Solvation Dynamics ◽  
Structured Systems ◽  
Reduced Viscosity ◽  
Bond Network ◽  
Fluorescent Molecules

<div><div><div><p>The structural features of a series of diverse Deep Eutectic Solvents (DESs) have been investigated and characterized by means of two fluorescent probes. The spectral and photophysical properties of the latter are strictly dependent on the experienced environment, so that they can provide insights into the polarity, viscosity, hydrogen-bond network, and micro-heterogeneity of the various DESs.</p><p>In fact, the investigated DESs exhibit a variety of properties with regards to their hydrophilicity, acidity, and hydrogen-bond ability, and these details were deeply probed by the two fluorescent molecules. The effect of the addition of water, which is a key strategy for tuning the properties of these structured systems, was also tested. In particular, the excited state dynamics of the probes, measured by femtosecond-resolved transient absorption, proved instrumental in understanding the changes in the structural properties of the DESs, namely reduced viscosity and enhanced heterogeneity, as the water percentage increases. Differences between the various DESs in terms of both local microheterogeneity and bulk viscosity also emerged from the peculiar multi-exponential solvation dynamics undergone by the excited states of the probes.</p></div></div></div>

Simulation of continuous random process according to the specified sequence of extremes

2020 ◽  
Vol 86 (7) ◽  
pp. 65-71
Author(s):  
I. V. Gadolina ◽  
R. I. Zainetdinov ◽  
T. P. Gryzlova ◽  
I. M. Petrova
Keyword(s):  
Spectral Density ◽  
Random Process ◽  
Continuous Process ◽  
Continuous Processes ◽  
Regression Formula ◽  
Bending Stresses ◽  
Development And Validation ◽  
Irregular Loading ◽  
Cosine Functions ◽  
Maximum Minimum

A method has been developed for converting a discrete sequence of extrema into a continuous process. The relevancy of the problem is attributed to the necessity of an approximate estimation of spectral density in in testing materials and structures under random (irregular) loading. A great bulk of available experimental data thus can be used in development and validation of calculation methods for assessing durability in the multi-cycle region. Postulating the continuity of random stress processes and their first derivative we propose to connect piecewise the available starting points (namely, the extrema of the random process) with half-cosine functions under the condition of compatibility at the points of extrema. A distinctive feature of the method is the provision of 100% coincidence of the values and sequences of extrema in the initial discrete and simulated continuous processes. The issue of choosing the magnitude of half-periods for these half-cosine functions is addressed on the basis of information obtained from the analysis of real stress records in the form of a regression equation linking half-periods and half-ranges for some realizations of the random process for transport vehicles. The regression dependences of the half-periods and semi-ranges of bending stresses (part of a railway train) and torsion (torsion shaft of a tracked vehicle) are shown as an example. An analysis of the correlation of two random variables (half-periods and half-ranges) according to empirical data has shown that the correlation exists and is significant for the observed number of points thus providing the basis for using the regression formula for an approximate choice of the frequency composition of the process. Moreover, the lower restrictions are imposed on the number of points (at least 5) in the half-period. Since the extrema of the initial and simulated processes coincide in accordance with the principle of the proposed simulation, the distribution of the amplitudes of complete cycles, as well as the results of schematization by other known methods are identical, therefore, the estimate of the durability by hypotheses based on a linear one is also identical. The validation of the method consists in consideration of the chain: 1) the initial continuous process; 2) the discrete process of extrema; 3) simulated continuous process according to the proposed method. Auxiliary distributions, such as distributions of maximum, minimum and average cycle values also coincide in accordance with the principle of modeling. The method is proposed to be used in analysis of the comparability of two competing approaches in assessing the loading in the problems of assessing durability, namely: those that use cycle-counting methods and methods based on the spectral density of processes. Since the spectral densities of the processes can differ due to an approximate choice of the frequencies on the basis of a regression formula, methods on their base can give estimates of the durability that differ from those obtained by schematization methods. To study this phenomenon, further computational experiments are required. The developed method can be very useful for the experiment design.

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