Features of the milk whey protein partitioning in polyethyleneglycol-sodium citrate aqueous two-phase systems with the goal of isolating human alpha-1 antitrypsin expressed in bovine milk

2006 ◽  
Vol 837 (1-2) ◽  
pp. 18-23 ◽  
Author(s):  
Andrea Boaglio ◽  
Georgina Bassani ◽  
Guillermo Picó ◽  
Bibiana Nerli
Keyword(s):  
Whey Protein ◽  
Sodium Citrate ◽  
Bovine Milk ◽  
Two Phase ◽  
Milk Whey ◽  
Aqueous Two Phase Systems ◽  
Protein Partitioning ◽  
Phase Systems ◽  
Alpha 1 Antitrypsin ◽  
Milk Whey Protein

Study of the interactions between rosmarinic acid and bovine milk whey protein α-Lactalbumin, β-Lactoglobulin and Lactoferrin

2015 ◽  
Vol 77 ◽  
pp. 450-459 ◽  
Author(s):  
Vincenza Ferraro ◽  
Ana Raquel Madureira ◽  
Bruno Sarmento ◽  
Ana Gomes ◽  
Manuela E. Pintado
Keyword(s):  
Whey Protein ◽  
Rosmarinic Acid ◽  
Bovine Milk ◽  
Milk Whey ◽  
Β Lactoglobulin ◽  
Milk Whey Protein

Electrostatic potentials and protein partitioning in aqueous two-phase systems

AIChE Journal ◽  
1991 ◽  
Vol 37 (9) ◽  
pp. 1401-1409 ◽  
Author(s):  
C. A. Haynes ◽  
J. Carson ◽  
H. W. Blanch ◽  
J. M. Prausnitz
Keyword(s):  
Electrostatic Potentials ◽  
Two Phase ◽  
Aqueous Two Phase Systems ◽  
Protein Partitioning ◽  
Phase Systems

scholarly journals Phase Separation Behavior and System Properties of Aqueous Two-Phase Systems with Polyethylene Glycol and Different Salts: Experiment and Correlation

2015 ◽  
Vol 2015 ◽  
pp. 1-10 ◽  
Author(s):  
Haihua Yuan ◽  
Yang Liu ◽  
Wanqian Wei ◽  
Yongjie Zhao
Keyword(s):  
Phase Separation ◽  
Sodium Citrate ◽  
Line Length ◽  
Continuous Phase ◽  
Two Phase ◽  
Separation Rate ◽  
Aqueous Two Phase Systems ◽  
Phase Systems ◽  
System Properties ◽  
Tie Line

The phase separation behaviors of PEG1000/sodium citrate, PEG4000/sodium citrate, PEG1000/ammonium sulfate, and PEG4000/ammonium sulfate aqueous two-phase systems were investigated, respectively. There are two distinct situations for the phase separation rate in the investigated aqueous two-phase systems: one state is top-continuous phase with slow phase separation rate and strong bottom-continuous phase with fast phase separation rate and weak volume ratio dependence. The system properties such as density, viscosity, and interfacial tension between top and bottom phases which have effects on the phase separation rate of aqueous two-phase systems were measured. The property parameter differences between the two phases increased with increasing tie line length and then improved the phase separation rate. Moreover, a modified correlation equation including the phase separation rate, tie line length, and physical properties of the four aqueous two-phase systems has been proposed and successfully tested in the bottom-continuous phase, whose coefficients were estimated through regression analysis. The predicted results of PEG1000/sodium citrate aqueous two-phase systems were verified through the stationary phase retention in the cross-axis countercurrent chromatography.

Ternary and binary deep eutectic solvents as a novel extraction medium for protein partitioning

2016 ◽  
Vol 8 (46) ◽  
pp. 8196-8207 ◽  
Author(s):  
Hongmei Zhang ◽  
Yuzhi Wang ◽  
Kaijia Xu ◽  
Na Li ◽  
Qian Wen ◽  
...  
Keyword(s):  
Deep Eutectic Solvents ◽  
Two Phase ◽  
Aqueous Two Phase Systems ◽  
Protein Partitioning ◽  
Phase Systems

This study was devoted to compare ternary and binary deep eutectic solvents (DESs) with aqueous two-phase systems (ATPSs) to extract proteins.

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