Abstract
Background
It is well known that Autoimmune thyroid disease is multifactorial with multiple genetic and environmental factors, immune malfunction also incriminated in the development of this disease, The exact pathogenesis of this disease remains unclear despite the fact that the production of autoantibodies destroys self-tolerance and agitate the adaptive immune system. Our study will answer the question is there a difference in Toll like receptor 9 (TLR 9) expression in peripheral blood mononuclear cell (PBMCs) from Grave’s disease patients.
Objective
to measure TLR9 percentage expression on peripheral blood mononuclear cells in patients with Graves’ disease.
Methods
60 subjects were included in this study; 30 with Graves’ disease and 30 healthy individuals as control group. All the patients were subjected to the following: Full history, clinical examination, thyroid functions, Thyroid ultrasound, Radioisotope thyroid scan: to assess uptake of thyroid gland and Toll like receptor 9 (TLR 9) percentage expression on peripheral blood mononuclear cells will be analyzed using flow cytometry technique.
Results
The present study proved that patients with Graves’ disease had higher levels of percentage expression of TLR 9 on peripheral blood lymphocytes.
Conclusion
percentage expression of TLR9 on peripheral blood lymphocytes is higher in Graves’ patients.
Development and Validation of Assays for the Quantification of β-D-N4-hydroxycytidine in Human Plasma and β-D-N4-hydroxycytidine-Triphosphate in Peripheral Blood Mononuclear Cell Lysates
