Peripheral blood mononuclear cell subsets in patients with severe inherited forms of epidermolysis bullosa

Abstract Background It is well known that Autoimmune thyroid disease is multifactorial with multiple genetic and environmental factors, immune malfunction also incriminated in the development of this disease, The exact pathogenesis of this disease remains unclear despite the fact that the production of autoantibodies destroys self-tolerance and agitate the adaptive immune system. Our study will answer the question is there a difference in Toll like receptor 9 (TLR 9) expression in peripheral blood mononuclear cell (PBMCs) from Grave’s disease patients. Objective to measure TLR9 percentage expression on peripheral blood mononuclear cells in patients with Graves’ disease. Methods 60 subjects were included in this study; 30 with Graves’ disease and 30 healthy individuals as control group. All the patients were subjected to the following: Full history, clinical examination, thyroid functions, Thyroid ultrasound, Radioisotope thyroid scan: to assess uptake of thyroid gland and Toll like receptor 9 (TLR 9) percentage expression on peripheral blood mononuclear cells will be analyzed using flow cytometry technique. Results The present study proved that patients with Graves’ disease had higher levels of percentage expression of TLR 9 on peripheral blood lymphocytes. Conclusion percentage expression of TLR9 on peripheral blood lymphocytes is higher in Graves’ patients.

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Chemotherapy-induced fatigue is associated with changes in gene expression in the peripheral blood mononuclear cell fraction of patients with locoregional breast cancer

Supportive Care in Cancer ◽

10.1007/s00520-018-4519-0 ◽

2018 ◽

Vol 27 (7) ◽

pp. 2479-2486 ◽

Cited By ~ 2

Author(s):

Barbara Bonaparte Ribas de Alcântara ◽

Felipe Melo Cruz ◽

Fernando Luiz Affonso Fonseca ◽

Beatriz da Costa Aguiar Alves ◽

Matheus Moreira Perez ◽

...

Keyword(s):

Breast Cancer ◽

Gene Expression ◽

Peripheral Blood Mononuclear Cell ◽

Mononuclear Cell ◽

Peripheral Blood ◽

Cell Fraction ◽

Peripheral Blood Mononuclear ◽

Mononuclear Cell Fraction

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A Module of Human Peripheral Blood Mononuclear Cell Transcriptional Network Containing Primitive and Differentiation Markers Is Related to Specific Cardiovascular Health Variables

PLoS ONE ◽

10.1371/journal.pone.0095124 ◽

2014 ◽

Vol 9 (4) ◽

pp. e95124 ◽

Cited By ~ 4

Author(s):

Leni Moldovan ◽

Mirela Anghelina ◽

Taylor Kantor ◽

Desiree Jones ◽

Enass Ramadan ◽

...

Keyword(s):

Peripheral Blood Mononuclear Cell ◽

Mononuclear Cell ◽

Peripheral Blood ◽

Cardiovascular Health ◽

Human Peripheral Blood ◽

Transcriptional Network ◽

Differentiation Markers ◽

Peripheral Blood Mononuclear

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Avidin inhibits PHA-induced human peripheral blood mononuclear cell proliferation

Medical Journal of Indonesia ◽

10.13181/mji.v25i1.1264 ◽

2016 ◽

Vol 25 (1) ◽

pp. 19-24

Author(s):

Cicia Firakania ◽

Indra G. Mansur ◽

Sri W.A. Jusman ◽

Mohamad Sadikin

Keyword(s):

Cell Cycle ◽

Cell Proliferation ◽

Peripheral Blood Mononuclear Cell ◽

Mononuclear Cell ◽

Peripheral Blood ◽

De Novo ◽

Human Peripheral Blood ◽

Interleukin 2 ◽

Peripheral Blood Mononuclear ◽

Cell Proliferation Inhibition

Background: Cell proliferation occurs not only in normal but also in cancer cells. Most of cell proliferation inhibition can be done by inhibiting the DNA synthesis, notably by intervening the formation of purine or pyrimidine. In purine de novo synthesis, it was assumed that biotin plays a role as a coenzyme in carboxylation reaction, one of the pivotal steps in the purine de novo pathways. The aim of this study was to see the avidin potency to bind biotin and inhibit mitosis.Methods: Peripheral blood mononuclear cell (PBMC) was cultured in RPMI-1640 medium and stimulated by phytohemagglutinin (PHA) in the presence or absence of interleukin-2 (IL-2), with or without avidin. The effect of avidin addition was observed at 24, 48, and 72 hours for cell proliferation, viability, and cell cycle. Statistical analysis was done by one-way ANOVA.Results: Avidin inhibited cell proliferation and viability in culture under stimulation by PHA with and without IL-2. Cell cycle analysis showed that avidin arrested the progression of PBMC after 72 hours of culture. Most cells were found in G0/G1 phase.Conclusion: Inhibition of biotin utilization by avidin binding can halt cell proliferation.

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