Mechanistic profiling of the release kinetics of siRNA from lipidoid-polymer hybrid nanoparticles in vitro and in vivo after pulmonary administration

2019 ◽  
Vol 310 ◽  
pp. 82-93 ◽  
Author(s):  
Kaushik Thanki ◽  
Delphine van Eetvelde ◽  
Antonia Geyer ◽  
Juan Fraire ◽  
Remi Hendrix ◽  
...  
Keyword(s):  
Release Kinetics ◽  
Hybrid Nanoparticles ◽  
Pulmonary Administration ◽  
Polymer Hybrid ◽  
Kinetics Of

Two-component reduction-sensitive lipid–polymer hybrid nanoparticles for triggered drug release and enhanced in vitro and in vivo anti-tumor efficacy

2017 ◽  
Vol 5 (1) ◽  
pp. 98-110 ◽  
Author(s):  
Liu-Jie Zhang ◽  
Bo Wu ◽  
Wei Zhou ◽  
Cai-Xia Wang ◽  
Qian Wang ◽  
...  
Keyword(s):  
Drug Release ◽  
Hybrid Nanoparticles ◽  
Polymer Hybrid ◽  
Two Component ◽  
Triggered Drug Release

Two-component reduction-sensitive lipid–polymer hybrid nanoparticles composed of DLPE-S-S-MPEG and PCL were developed for intracellular reduction triggered delivery of DOX.

scholarly journals Drug Release Kinetics of Electrospun PHB Meshes

Materials ◽  
2019 ◽  
Vol 12 (12) ◽  
pp. 1924 ◽  
Author(s):  
Vojtech Kundrat ◽  
Nicole Cernekova ◽  
Adriana Kovalcik ◽  
Vojtech Enev ◽  
Ivana Marova
Keyword(s):  
Release Kinetics ◽  
Entrapment Efficiency ◽  
Toxic Substances ◽  
Antimicrobial Efficiency ◽  
Vis Spectroscopy ◽  
Model Drug ◽  
Phosphate Buffered Saline ◽  
Kinetics Of

Microbial poly(3-hydroxybutyrate) (PHB) has several advantages including its biocompatibility and ability to degrade in vivo and in vitro without toxic substances. This paper investigates the feasibility of electrospun PHB meshes serving as drug delivery systems. The morphology of the electrospun samples was modified by varying the concentration of PHB in solution and the solvent composition. Scanning electron microscopy of the electrospun PHB scaffolds revealed the formation of different morphologies including porous, filamentous/beaded and fiber structures. Levofloxacin was used as the model drug for incorporation into PHB electrospun meshes. The entrapment efficiency was found to be dependent on the viscosity of the PHB solution used for electrospinning and ranged from 14.4–81.8%. The incorporation of levofloxacin in electrospun meshes was confirmed by Fourier-transform infrared spectroscopy and UV-VIS spectroscopy. The effect of the morphology of the electrospun meshes on the levofloxacin release profile was screened in vitro in phosphate-buffered saline solution. Depending upon the morphology, the electrospun meshes released about 14–20% of levofloxacin during the first 24 h. The percentage of drug released after 13 days increased up to 32.4% and was similar for all tested morphologies. The antimicrobial efficiency of all tested samples independent of the morphology, was confirmed by agar diffusion testing.

scholarly journals pH Sensitive Lipid-Polymer Hybrid Nanoparticles Mediated Delivery of Docetaxel: A Viable Approach for Breast Cancer Therapeutic Intervention Development

2020 ◽  
Author(s):  
Ye Yuan ◽  
Jia-Xing Song ◽  
Mei-Na Zhang ◽  
Baoshan Yuan
Keyword(s):  
Breast Cancer ◽  
Cellular Uptake ◽  
Intervention Development ◽  
Ph Sensitive ◽  
Therapeutic Interventions ◽  
Hybrid Nanoparticles ◽  
Breast Cancer Cell Lines ◽  
Polymer Hybrid

Abstract Present study was planned for the development of pH sensitive lipid polymer hybrid nanoparticles (pHS-LPHNPs) loaded with docetaxel (DTX) for guided and target specific cytosolic-delivery delivery of docetaxel (DTX). pHS-LPHNPs were formulated to entrap DTX by self-assembled nano-precipitation technique and characterised with respect to zeta potential, particle-size, entrapment efficiency, PDI as well as invitro drug release. The cell viability, apoptosis, cellular-uptake, pharmacokinetics, bio-distribution in vital organs, % changes in tumour volume and survival of breast cancer bearing animals were used for the evaluation of efficacy of the formulation. In-vitro studies showed increased cytotoxicity at lower IC50 and better cellular-uptake of pHS-LPHNPs mediated drug by breast cancer cell lines. We saw the better rate of apoptosis of breast cancer cells via Annexin V/Propidium iodide staining. Moreover, in-vivo studies demonstrated improved pharmacokinetics and targetability with minimum drug circulation in deep-seated organs upon delivery of DTX via pHS-LPHNPs in comparison with LPHNPs-DTX and free DTX. We observed sizeable % reduction in tumour-burden with pHS-LPHNPs-DTXthan that withLPHNPs-DTX &free DTX. In brief, pHS-LPHNPs mediated delivery of DTX exhibited promising approach for developing therapeutic-interventions against breast-cancer.

scholarly journals Codelivery of doxorubicin and triptolide with reduction-sensitive lipid–polymer hybrid nanoparticles for in vitro and in vivo synergistic cancer treatment

2017 ◽  
Vol Volume 12 ◽  
pp. 1853-1862 ◽  
Author(s):  
Bo Wu ◽  
Shu-Ting Lu ◽  
Liu-Jie Zhang ◽  
Ren-Xi Zhuo ◽  
Hai-Bo Xu ◽  
...  
Keyword(s):  
Cancer Treatment ◽  
Hybrid Nanoparticles ◽  
Polymer Hybrid

Local anesthetic effects of bupivacaine loaded lipid-polymer hybrid nanoparticles: In vitro and in vivo evaluation

2017 ◽  
Vol 89 ◽  
pp. 689-695 ◽  
Author(s):  
Pengju Ma ◽  
Ting Li ◽  
Huaixin Xing ◽  
Suzhen Wang ◽  
Yingui Sun ◽  
...  
Keyword(s):  
Local Anesthetic ◽  
Hybrid Nanoparticles ◽  
In Vivo Evaluation ◽  
Polymer Hybrid

scholarly journals Development of Lipid–Polymer Hybrid Nanoparticles for Improving Oral Absorption of Enoxaparin

Pharmaceutics ◽  
2020 ◽  
Vol 12 (7) ◽  
pp. 607
Author(s):  
Bo Tang ◽  
Yu Qian ◽  
Guihua Fang
Keyword(s):  
Oral Delivery ◽  
Encapsulation Efficiency ◽  
Oral Absorption ◽  
Poloxamer 407 ◽  
Hybrid Nanoparticles ◽  
Polymer Hybrid ◽  
Study Results ◽  
Confocal Scanning

Enoxaparin, an anticoagulant that helps prevent the formation of blood clots, is administered parenterally. Here, we report the development and evaluation of lipid–polymer hybrid nanoparticles (LPHNs) for the oral delivery of enoxaparin. The polymer poloxamer 407 (P407) was incorporated into lipid nanoparticles to form gel cores and ensure high encapsulation efficiency and the controlled release of enoxaparin. In vitro results indicated that 30% of P407 incorporation offered higher encapsulation efficiency and sustained the release of enoxaparin. Laser confocal scanning microscopy (LCSM) images showed that LPHNs could not only significantly improve the accumulation of enoxaparin in intestinal villi but also facilitate enoxaparin transport into the underlayer of intestinal epithelial cells. In vivo pharmacokinetic study results indicated that the oral bioavailability of enoxaparin was markedly increased about 6.8-fold by LPHNs. In addition, its therapeutic efficacy against pulmonary thromboembolism was improved 2.99-fold by LPHNs. Moreover, LPHNs exhibited excellent biocompatibility in the intestine. Overall, the LPHN is a promising delivery carrier to boost the oral absorption of enoxaparin.

scholarly journals Development of Sustained Release Baricitinib Loaded Lipid-Polymer Hybrid Nanoparticles with Improved Oral Bioavailability

Molecules ◽  
2021 ◽  
Vol 27 (1) ◽  
pp. 168
Author(s):  
Md. Khalid Anwer ◽  
Essam A. Ali ◽  
Muzaffar Iqbal ◽  
Mohammed Muqtader Ahmed ◽  
Mohammed F. Aldawsari ◽  
...  
Keyword(s):  
Particle Size ◽  
Sustained Release ◽  
In Vitro Release ◽  
Entrapment Efficiency ◽  
Janus Kinase ◽  
Hybrid Nanoparticles ◽  
Polymer Hybrid ◽  
Vitro Release

Baricitinib (BTB) is an orally administered Janus kinase inhibitor, therapeutically used for the treatment of rheumatoid arthritis. Recently it has also been approved for the treatment of COVID-19 infection. In this study, four different BTB-loaded lipids (stearin)-polymer (Poly(d,l-lactide-co-glycolide)) hybrid nanoparticles (B-PLN1 to B-PLN4) were prepared by the single-step nanoprecipitation method. Next, they were characterised in terms of physicochemical properties such as particle size, zeta potential (ζP), polydispersity index (PDI), entrapment efficiency (EE) and drug loading (DL). Based on preliminary evaluation, the B-PLN4 was regarded as the optimised formulation with particle size (272 ± 7.6 nm), PDI (0.225), ζP (−36.5 ± 3.1 mV), %EE (71.6 ± 1.5%) and %DL (2.87 ± 0.42%). This formulation (B-PLN4) was further assessed concerning morphology, in vitro release, and in vivo pharmacokinetic studies in rats. The in vitro release profile exhibited a sustained release pattern well-fitted by the Korsmeyer–Peppas kinetic model (R2 = 0.879). The in vivo pharmacokinetic data showed an enhancement (2.92 times more) in bioavailability in comparison to the normal suspension of pure BTB. These data concluded that the formulated lipid-polymer hybrid nanoparticles could be a promising drug delivery option to enhance the bioavailability of BTB. Overall, this study provides a scientific basis for future studies on the entrapment efficiency of lipid-polymer hybrid systems as promising carriers for overcoming pharmacokinetic limitations.

Crosslinked Chitosan/PVA Film, Suturated with 5- Fluorouracil for The Prevention of Proliferative Vitreoretinopathy

2016 ◽  
Vol 6 (2) ◽  
Author(s):  
Baiyrkhanova A. ◽  
Ismailova A. ◽  
Botabekova T. ◽  
Enin E. ◽  
Semenova Y.
Keyword(s):  
Proliferative Vitreoretinopathy ◽  
Release Kinetics ◽  
Drug Loading ◽  
In Vitro Release ◽  
Chemical Compositions ◽  
Ophthalmic Administration ◽  
Model Drug ◽  
Kinetics Of

5-Fluorouracil (5-FU)-loaded chitosan (Ch) film for chemotherapy were prepared applying a superhydrophobic surfacebased encapsulation technology. The aim of this study was to develop polymeric film with glutaraldehyde (GA) of controlled drug delivery systems for 5 – fluorouracil (FU) as a model drug for the treatment of proliferative vitreoretinopathy. Polymer film of chitosan and polyvinyl alcohol (PVA in 75:25 ratios were prepared and treated with GA. FTIR spectra of 5-FU, Ch/5-FU and Ch/PVA film loaded 5-FU were studied. Physical characteristics such as thickness and swelling coefficient of the film were performed. The thermal of the Ch/PVA film was studied with thermogravimethric analysis. The drug loading efficiency, film size and chemical compositions of the film loaded drug were confirmed by UV–vis spectrophotometer and Fourier transform infrared spectroscopy. In vitro release kinetics of drug from the polymeric films was investigated to determine the drug release properties. In vivo study of PVR was showed the efficacy and no toxicity of this formulation. Further uses of the film loaded 5 - fluorouracil may provide an efficiency deliverable for ophthalmic administration.

Folate-containing reduction-sensitive lipid–polymer hybrid nanoparticles for targeted delivery of doxorubicin

2015 ◽  
Vol 3 (4) ◽  
pp. 655-664 ◽  
Author(s):  
Bo Wu ◽  
Ping Yu ◽  
Can Cui ◽  
Ming Wu ◽  
Yang Zhang ◽  
...  
Keyword(s):  
Drug Delivery ◽  
Outer Layer ◽  
Targeted Delivery ◽  
Hybrid Nanoparticles ◽  
Polymer Hybrid

Lipid–polymer hybrid nanoparticles (FLPNPs) containing a folate targeted ligand and a reduction-sensitive outer layer were developed to enhance drug delivery efficacy in vitro and in vivo.

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